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Decrease in intramuscular lipid droplets and translocation of HSL in response to muscle contraction and epinephrine

Prats, Clara ; Donsmark, Morten ; Qvortrup, Klaus ; Londos, Constantine ; Sztalryd, Carole ; Holm, Cecilia LU ; Galbo, Henrik and Ploug, Thorkil (2006) In Journal of Lipid Research 47(11). p.2392-2399
Abstract
A better understanding of skeletal muscle lipid metabolism is needed to identify the molecular mechanisms relating intramuscular triglyceride (IMTG) to muscle metabolism and insulin sensitivity. An increasing number of proteins have been reported to be associated with intracellular triglyceride (TG), among them the PAT family members: perilipin, ADRP ( for adipocyte differentiation-related protein), and TIP47 ( for tail-interacting protein of 47 kDa). Hormone-sensitive lipase (HSL) is thought to be the major enzyme responsible for IMTG hydrolysis in skeletal muscle. In adipocytes, regulation of HSL by intracellular redistribution has been demonstrated. The existence of such regulatory mechanisms in skeletal muscle has long been... (More)
A better understanding of skeletal muscle lipid metabolism is needed to identify the molecular mechanisms relating intramuscular triglyceride (IMTG) to muscle metabolism and insulin sensitivity. An increasing number of proteins have been reported to be associated with intracellular triglyceride (TG), among them the PAT family members: perilipin, ADRP ( for adipocyte differentiation-related protein), and TIP47 ( for tail-interacting protein of 47 kDa). Hormone-sensitive lipase (HSL) is thought to be the major enzyme responsible for IMTG hydrolysis in skeletal muscle. In adipocytes, regulation of HSL by intracellular redistribution has been demonstrated. The existence of such regulatory mechanisms in skeletal muscle has long been hypothesized but has never been demonstrated. The aim of this study was to characterize the PAT family proteins associated with IMTG and to investigate the effect of epinephrine stimulation or muscle contraction on skeletal muscle TG content and HSL intracellular distribution. Rat soleus muscles were either incubated with epinephrine or electrically stimulated for 15 min. Single muscle fibers were used for morphological analysis by confocal and transmission electron microscopy. We show a decrease in IMTG in response to both lipolytic stimuli. Furthermore, we identify two PAT family proteins, ADRP and TIP47, associated with IMTG. Finally, we demonstrate HSL translocation to IMTG and ADRP after stimulation with epinephrine or contraction. (Less)
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author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
adipocyte differentiation-related protein, hormone-sensitive lipase, lipid metabolism regulation, skeletal muscle, 47 kDa, tail-interacting protein of
in
Journal of Lipid Research
volume
47
issue
11
pages
2392 - 2399
publisher
American Society for Biochemistry and Molecular Biology
external identifiers
  • wos:000241667300005
  • scopus:33845590561
ISSN
1539-7262
DOI
10.1194/jlr.M600247-JLR200
language
English
LU publication?
yes
id
dc671bb3-a991-48eb-bfdd-5c66986d97f9 (old id 378802)
date added to LUP
2016-04-01 11:47:31
date last changed
2020-10-20 01:50:18
@article{dc671bb3-a991-48eb-bfdd-5c66986d97f9,
  abstract     = {A better understanding of skeletal muscle lipid metabolism is needed to identify the molecular mechanisms relating intramuscular triglyceride (IMTG) to muscle metabolism and insulin sensitivity. An increasing number of proteins have been reported to be associated with intracellular triglyceride (TG), among them the PAT family members: perilipin, ADRP ( for adipocyte differentiation-related protein), and TIP47 ( for tail-interacting protein of 47 kDa). Hormone-sensitive lipase (HSL) is thought to be the major enzyme responsible for IMTG hydrolysis in skeletal muscle. In adipocytes, regulation of HSL by intracellular redistribution has been demonstrated. The existence of such regulatory mechanisms in skeletal muscle has long been hypothesized but has never been demonstrated. The aim of this study was to characterize the PAT family proteins associated with IMTG and to investigate the effect of epinephrine stimulation or muscle contraction on skeletal muscle TG content and HSL intracellular distribution. Rat soleus muscles were either incubated with epinephrine or electrically stimulated for 15 min. Single muscle fibers were used for morphological analysis by confocal and transmission electron microscopy. We show a decrease in IMTG in response to both lipolytic stimuli. Furthermore, we identify two PAT family proteins, ADRP and TIP47, associated with IMTG. Finally, we demonstrate HSL translocation to IMTG and ADRP after stimulation with epinephrine or contraction.},
  author       = {Prats, Clara and Donsmark, Morten and Qvortrup, Klaus and Londos, Constantine and Sztalryd, Carole and Holm, Cecilia and Galbo, Henrik and Ploug, Thorkil},
  issn         = {1539-7262},
  language     = {eng},
  number       = {11},
  pages        = {2392--2399},
  publisher    = {American Society for Biochemistry and Molecular Biology},
  series       = {Journal of Lipid Research},
  title        = {Decrease in intramuscular lipid droplets and translocation of HSL in response to muscle contraction and epinephrine},
  url          = {http://dx.doi.org/10.1194/jlr.M600247-JLR200},
  doi          = {10.1194/jlr.M600247-JLR200},
  volume       = {47},
  year         = {2006},
}