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Treatment of tumour cell with 5-aza-2-deoxycytidine (DAC) for immune tumour therapy of Glioma in Fischer-344 rats

Persson, Bertil R LU ; Engström, Per LU ; Grafström, Gustav LU ; Baureus Koch, Catrin LU ; Widegren, Bengt LU and Salford, Leif LU (2012) In Acta Scientiarum Lundensia 2012(007). p.1-21
Abstract
Fisher 344 rats with implanted N29 glioma tumours were treated with Pulsed

Electric Fields (PEF) in combination with immunization using either IFN-gamma-gene-transfected syngeneic tumour cells or IFN-gamma transfected N29 cells

treated with 10 micro-M 5-aza-2-deoxycytidine (DAC).

Tumours (N29) were inoculated subcutaneously on both thighs of female F-

344 syngeneic rats. The left tumour was treated once with 16 exponential

pulses with an electric field strength of 1400 V/cm, and 1.0 ms duration (time constant). No anticancer drugs were given at any time. The following day and then once weekly for three weeks, the animals were given intra-peritoneal

injections of irradiated, modified N29... (More)
Fisher 344 rats with implanted N29 glioma tumours were treated with Pulsed

Electric Fields (PEF) in combination with immunization using either IFN-gamma-gene-transfected syngeneic tumour cells or IFN-gamma transfected N29 cells

treated with 10 micro-M 5-aza-2-deoxycytidine (DAC).

Tumours (N29) were inoculated subcutaneously on both thighs of female F-

344 syngeneic rats. The left tumour was treated once with 16 exponential

pulses with an electric field strength of 1400 V/cm, and 1.0 ms duration (time constant). No anticancer drugs were given at any time. The following day and then once weekly for three weeks, the animals were given intra-peritoneal

injections of irradiated, modified N29 tumour cells.

The results were evaluated by daily measuring the size of tumour on both

sides of the animals. Treatment with solely PEF in 32 animals resulted in a

specific growth rate decrease of 20±6 % on the PEF exposed tumour. The

effect at the non targeted tumour was negligible (0±4 %). Treatment with

IFN-gamma secreting tumour cells resulted in a significant decrease of tumour

growth rate on the right tumour of 20± 2 % (p< 0.05) and no significant

effect (3±0.3% ) was observed on the left tumour. Immunization with DAC

treated IFN-gamma secreting cells in 12 animals showed no significant decreased

growth rate, on neither the left nor the right tumours.

By combining PEF+IFN-gamma no significant decrease in growth rate was achieved.

But in the combination of PEF and IFN-gamma secreting cells grown in DAC medium

the tumour growth rate decreased by about 50 % at the PEF treated tumour

and there was a decrease of about 20% in tumour growth at the non-PEF

treated tumour rate which is about the same as for PEF treatment alone.

Immune therapy of rats with intracranial N32 tumours by immunization with

IFN-gamma secreting syngeneic cells treated with DAC resulted in a slight (3%) but

not significant increase in survival time. With a single RT fraction of 15 Gy

there was, however, a significant increase of 32% in the length of survival

time of the rats with N32 tumours (p<0.02). Radiation therapy with a single

fraction of 15 Gy combined with immunization with IFN-gamma secreting syngeneic

cells treated with DAC resulted in significant (p<0.01) 34% increased length

of survival time for the N32 tumours although there were no complete

remissions. (Less)
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organization
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Contribution to journal
publication status
published
subject
keywords
RT, radiation therapy, glioma, Fischer rat, Abscopal, non-target, Tumor growth rate, TGR, Immunization, syngeneic tumor cells, interferon-gamma, IFNg.
in
Acta Scientiarum Lundensia
volume
2012
issue
007
pages
1 - 21
publisher
Bertil RR Persson, Medical Radiation Physics, 22185 Lund, Sweden
ISSN
1651-5013
language
English
LU publication?
yes
id
1048b9a4-6251-45db-a0fb-f3cc907cf87b (old id 3807515)
date added to LUP
2015-05-27 12:23:14
date last changed
2016-09-11 10:02:42
@article{1048b9a4-6251-45db-a0fb-f3cc907cf87b,
  abstract     = {Fisher 344 rats with implanted N29 glioma tumours were treated with Pulsed<br/><br>
Electric Fields (PEF) in combination with immunization using either IFN-gamma-gene-transfected syngeneic tumour cells or IFN-gamma transfected N29 cells<br/><br>
treated with 10 micro-M 5-aza-2-deoxycytidine (DAC).<br/><br>
Tumours (N29) were inoculated subcutaneously on both thighs of female F-<br/><br>
344 syngeneic rats. The left tumour was treated once with 16 exponential<br/><br>
pulses with an electric field strength of 1400 V/cm, and 1.0 ms duration (time constant). No anticancer drugs were given at any time. The following day and then once weekly for three weeks, the animals were given intra-peritoneal<br/><br>
injections of irradiated, modified N29 tumour cells.<br/><br>
The results were evaluated by daily measuring the size of tumour on both<br/><br>
sides of the animals. Treatment with solely PEF in 32 animals resulted in a<br/><br>
specific growth rate decrease of 20±6 % on the PEF exposed tumour. The<br/><br>
effect at the non targeted tumour was negligible (0±4 %). Treatment with<br/><br>
IFN-gamma secreting tumour cells resulted in a significant decrease of tumour<br/><br>
growth rate on the right tumour of 20± 2 % (p&lt; 0.05) and no significant<br/><br>
effect (3±0.3% ) was observed on the left tumour. Immunization with DAC<br/><br>
treated IFN-gamma secreting cells in 12 animals showed no significant decreased<br/><br>
growth rate, on neither the left nor the right tumours.<br/><br>
By combining PEF+IFN-gamma no significant decrease in growth rate was achieved.<br/><br>
But in the combination of PEF and IFN-gamma secreting cells grown in DAC medium<br/><br>
the tumour growth rate decreased by about 50 % at the PEF treated tumour<br/><br>
and there was a decrease of about 20% in tumour growth at the non-PEF<br/><br>
treated tumour rate which is about the same as for PEF treatment alone.<br/><br>
Immune therapy of rats with intracranial N32 tumours by immunization with<br/><br>
IFN-gamma secreting syngeneic cells treated with DAC resulted in a slight (3%) but<br/><br>
not significant increase in survival time. With a single RT fraction of 15 Gy<br/><br>
there was, however, a significant increase of 32% in the length of survival<br/><br>
time of the rats with N32 tumours (p&lt;0.02). Radiation therapy with a single<br/><br>
fraction of 15 Gy combined with immunization with IFN-gamma secreting syngeneic<br/><br>
cells treated with DAC resulted in significant (p&lt;0.01) 34% increased length<br/><br>
of survival time for the N32 tumours although there were no complete<br/><br>
remissions.},
  author       = {Persson, Bertil R and Engström, Per and Grafström, Gustav and Baureus Koch, Catrin and Widegren, Bengt and Salford, Leif},
  issn         = {1651-5013},
  keyword      = {RT,radiation therapy,glioma,Fischer rat,Abscopal,non-target,Tumor growth rate,TGR,Immunization,syngeneic tumor cells,interferon-gamma,IFNg.},
  language     = {eng},
  number       = {007},
  pages        = {1--21},
  publisher    = {Bertil RR Persson, Medical Radiation Physics, 22185 Lund, Sweden},
  series       = {Acta Scientiarum Lundensia},
  title        = {Treatment of tumour cell with 5-aza-2-deoxycytidine (DAC) for immune tumour therapy of Glioma in Fischer-344 rats},
  volume       = {2012},
  year         = {2012},
}