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Isolation and initial characterization of a novel zinc finger gene, DNMT3L, on 21q22.3, related to the cytosine-5-methyltransferase 3 gene family

Aapola, U; Shibuya, K; Scott, HS; Ollila, J; Vihinen, Mauno LU ; Heino, M; Shintani, A; Kawasaki, K; Minoshima, S and Krohn, K, et al. (2000) In Genomics 65(3). p.293-298
Abstract
We have isolated the DNMT3L gene that is related to the cytosine-5-methyltransferase 3 (DNMT3) family. The gene is located on chromosome 21q22.3 between the AIRE and the KIAA0653 genes and spans approximately 16 kb of genomic sequence. The encoded protein of 387 amino acids has a cysteine-rich region containing a novel-type zinc finger domain that is conserved in DNMT3A and DNMT3B but also in ATRX, a member of the SNF2 protein family, The novel domain, called an ADD (ATRX, DNMT3, DNMT3L)-type zinc finger, contains two subparts: a C2C2 and an imperfect PHD zinc finger. Expression of the DNMT3L mRNA was not detectable by Northern blotting; however, RT-PCR amplification revealed that it is expressed at low levels in several tissues including... (More)
We have isolated the DNMT3L gene that is related to the cytosine-5-methyltransferase 3 (DNMT3) family. The gene is located on chromosome 21q22.3 between the AIRE and the KIAA0653 genes and spans approximately 16 kb of genomic sequence. The encoded protein of 387 amino acids has a cysteine-rich region containing a novel-type zinc finger domain that is conserved in DNMT3A and DNMT3B but also in ATRX, a member of the SNF2 protein family, The novel domain, called an ADD (ATRX, DNMT3, DNMT3L)-type zinc finger, contains two subparts: a C2C2 and an imperfect PHD zinc finger. Expression of the DNMT3L mRNA was not detectable by Northern blotting; however, RT-PCR amplification revealed that it is expressed at low levels in several tissues including testis, ovary, and thymus. (C) 2000 Academic Press. (Less)
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type
Contribution to journal
publication status
published
subject
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Genomics
volume
65
issue
3
pages
293 - 298
publisher
Academic Press
external identifiers
  • wos:000087127500011
  • scopus:18744427037
ISSN
1089-8646
DOI
10.1006/geno.2000.6168
language
English
LU publication?
no
id
44690037-d1a7-4549-8119-fc2ba83a7c84 (old id 3852086)
date added to LUP
2013-06-28 13:14:56
date last changed
2017-10-01 03:45:12
@article{44690037-d1a7-4549-8119-fc2ba83a7c84,
  abstract     = {We have isolated the DNMT3L gene that is related to the cytosine-5-methyltransferase 3 (DNMT3) family. The gene is located on chromosome 21q22.3 between the AIRE and the KIAA0653 genes and spans approximately 16 kb of genomic sequence. The encoded protein of 387 amino acids has a cysteine-rich region containing a novel-type zinc finger domain that is conserved in DNMT3A and DNMT3B but also in ATRX, a member of the SNF2 protein family, The novel domain, called an ADD (ATRX, DNMT3, DNMT3L)-type zinc finger, contains two subparts: a C2C2 and an imperfect PHD zinc finger. Expression of the DNMT3L mRNA was not detectable by Northern blotting; however, RT-PCR amplification revealed that it is expressed at low levels in several tissues including testis, ovary, and thymus. (C) 2000 Academic Press.},
  author       = {Aapola, U and Shibuya, K and Scott, HS and Ollila, J and Vihinen, Mauno and Heino, M and Shintani, A and Kawasaki, K and Minoshima, S and Krohn, K and Antonarakis, SE and Shimizu, N and Kudoh, J and Peterson, P},
  issn         = {1089-8646},
  language     = {eng},
  number       = {3},
  pages        = {293--298},
  publisher    = {Academic Press},
  series       = {Genomics},
  title        = {Isolation and initial characterization of a novel zinc finger gene, DNMT3L, on 21q22.3, related to the cytosine-5-methyltransferase 3 gene family},
  url          = {http://dx.doi.org/10.1006/geno.2000.6168},
  volume       = {65},
  year         = {2000},
}