Isolation and initial characterization of a novel zinc finger gene, DNMT3L, on 21q22.3, related to the cytosine-5-methyltransferase 3 gene family
(2000) In Genomics 65(3). p.293-298- Abstract
- We have isolated the DNMT3L gene that is related to the cytosine-5-methyltransferase 3 (DNMT3) family. The gene is located on chromosome 21q22.3 between the AIRE and the KIAA0653 genes and spans approximately 16 kb of genomic sequence. The encoded protein of 387 amino acids has a cysteine-rich region containing a novel-type zinc finger domain that is conserved in DNMT3A and DNMT3B but also in ATRX, a member of the SNF2 protein family, The novel domain, called an ADD (ATRX, DNMT3, DNMT3L)-type zinc finger, contains two subparts: a C2C2 and an imperfect PHD zinc finger. Expression of the DNMT3L mRNA was not detectable by Northern blotting; however, RT-PCR amplification revealed that it is expressed at low levels in several tissues including... (More)
- We have isolated the DNMT3L gene that is related to the cytosine-5-methyltransferase 3 (DNMT3) family. The gene is located on chromosome 21q22.3 between the AIRE and the KIAA0653 genes and spans approximately 16 kb of genomic sequence. The encoded protein of 387 amino acids has a cysteine-rich region containing a novel-type zinc finger domain that is conserved in DNMT3A and DNMT3B but also in ATRX, a member of the SNF2 protein family, The novel domain, called an ADD (ATRX, DNMT3, DNMT3L)-type zinc finger, contains two subparts: a C2C2 and an imperfect PHD zinc finger. Expression of the DNMT3L mRNA was not detectable by Northern blotting; however, RT-PCR amplification revealed that it is expressed at low levels in several tissues including testis, ovary, and thymus. (C) 2000 Academic Press. (Less)
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https://lup.lub.lu.se/record/3852086
- author
- publishing date
- 2000
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Genomics
- volume
- 65
- issue
- 3
- pages
- 293 - 298
- publisher
- Academic Press
- external identifiers
-
- wos:000087127500011
- scopus:18744427037
- ISSN
- 1089-8646
- DOI
- 10.1006/geno.2000.6168
- language
- English
- LU publication?
- no
- id
- 44690037-d1a7-4549-8119-fc2ba83a7c84 (old id 3852086)
- date added to LUP
- 2016-04-01 12:00:07
- date last changed
- 2022-04-21 00:58:10
@article{44690037-d1a7-4549-8119-fc2ba83a7c84, abstract = {{We have isolated the DNMT3L gene that is related to the cytosine-5-methyltransferase 3 (DNMT3) family. The gene is located on chromosome 21q22.3 between the AIRE and the KIAA0653 genes and spans approximately 16 kb of genomic sequence. The encoded protein of 387 amino acids has a cysteine-rich region containing a novel-type zinc finger domain that is conserved in DNMT3A and DNMT3B but also in ATRX, a member of the SNF2 protein family, The novel domain, called an ADD (ATRX, DNMT3, DNMT3L)-type zinc finger, contains two subparts: a C2C2 and an imperfect PHD zinc finger. Expression of the DNMT3L mRNA was not detectable by Northern blotting; however, RT-PCR amplification revealed that it is expressed at low levels in several tissues including testis, ovary, and thymus. (C) 2000 Academic Press.}}, author = {{Aapola, U and Shibuya, K and Scott, HS and Ollila, J and Vihinen, Mauno and Heino, M and Shintani, A and Kawasaki, K and Minoshima, S and Krohn, K and Antonarakis, SE and Shimizu, N and Kudoh, J and Peterson, P}}, issn = {{1089-8646}}, language = {{eng}}, number = {{3}}, pages = {{293--298}}, publisher = {{Academic Press}}, series = {{Genomics}}, title = {{Isolation and initial characterization of a novel zinc finger gene, DNMT3L, on 21q22.3, related to the cytosine-5-methyltransferase 3 gene family}}, url = {{http://dx.doi.org/10.1006/geno.2000.6168}}, doi = {{10.1006/geno.2000.6168}}, volume = {{65}}, year = {{2000}}, }