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Microsatellite markers for powdery mildew resistance in pea (Pisum sativum L.)

Ek, M.; Eklund, M.; Von Post, R.; Dayteg, C.; Henriksson, T.; Weibull, P.; Ceplitis, Alf LU ; Isaac, P. and Tuvesson, S. (2005) In Hereditas 142. p.86-91
Abstract
Powdery mildew is a common disease of field pea, Pisum sativum L., and is caused by the ascomycete fungus Erysiphe pisi. It can cause severe damage in areas where pea is cultivated. Today breeders want to develop new pea lines that are resistant to the disease. To make the breeding process more efficient, it is desirable to find genetic markers for use in a marker-assisted selection (MAS) strategy. In this study, microsatellites (SSR) were used to find markers linked to powdery mildew resistance. The resistant pea cultivar '955180' and the susceptible pea cultivar 'Majoret' were crossed and F-2 plants were screened with SSR markers, using bulked segregant analysis. A total of 315 SSR markers were screened out of which five showed linkage... (More)
Powdery mildew is a common disease of field pea, Pisum sativum L., and is caused by the ascomycete fungus Erysiphe pisi. It can cause severe damage in areas where pea is cultivated. Today breeders want to develop new pea lines that are resistant to the disease. To make the breeding process more efficient, it is desirable to find genetic markers for use in a marker-assisted selection (MAS) strategy. In this study, microsatellites (SSR) were used to find markers linked to powdery mildew resistance. The resistant pea cultivar '955180' and the susceptible pea cultivar 'Majoret' were crossed and F-2 plants were screened with SSR markers, using bulked segregant analysis. A total of 315 SSR markers were screened out of which five showed linkage to the powdery mildew resistance gene. No single marker was considered optimal for inclusion in a MAS program. Instead, two of the markers can be used in combination, which would result in only 1.6% incorrectly identified plants. Thus SSR markers can be successfully used in marker-assisted selection for powdery mildew resistance breeding in pea. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Hereditas
volume
142
pages
86 - 91
publisher
Wiley-Blackwell
external identifiers
  • wos:000241491100014
  • pmid:16970617
  • scopus:33646037364
ISSN
1601-5223
DOI
10.1111/j.1601-5223.2005.01906.x
language
English
LU publication?
yes
id
350d11f3-01b5-46e6-aef9-4b166c3e89e9 (old id 386761)
date added to LUP
2007-08-17 15:55:16
date last changed
2017-07-09 03:46:36
@article{350d11f3-01b5-46e6-aef9-4b166c3e89e9,
  abstract     = {Powdery mildew is a common disease of field pea, Pisum sativum L., and is caused by the ascomycete fungus Erysiphe pisi. It can cause severe damage in areas where pea is cultivated. Today breeders want to develop new pea lines that are resistant to the disease. To make the breeding process more efficient, it is desirable to find genetic markers for use in a marker-assisted selection (MAS) strategy. In this study, microsatellites (SSR) were used to find markers linked to powdery mildew resistance. The resistant pea cultivar '955180' and the susceptible pea cultivar 'Majoret' were crossed and F-2 plants were screened with SSR markers, using bulked segregant analysis. A total of 315 SSR markers were screened out of which five showed linkage to the powdery mildew resistance gene. No single marker was considered optimal for inclusion in a MAS program. Instead, two of the markers can be used in combination, which would result in only 1.6% incorrectly identified plants. Thus SSR markers can be successfully used in marker-assisted selection for powdery mildew resistance breeding in pea.},
  author       = {Ek, M. and Eklund, M. and Von Post, R. and Dayteg, C. and Henriksson, T. and Weibull, P. and Ceplitis, Alf and Isaac, P. and Tuvesson, S.},
  issn         = {1601-5223},
  language     = {eng},
  pages        = {86--91},
  publisher    = {Wiley-Blackwell},
  series       = {Hereditas},
  title        = {Microsatellite markers for powdery mildew resistance in pea (Pisum sativum L.)},
  url          = {http://dx.doi.org/10.1111/j.1601-5223.2005.01906.x},
  volume       = {142},
  year         = {2005},
}