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Evaluation of progesterone content in saliva using magnetic particle-based immuno supported liquid membrane assay (m-ISLMA)

Tudorache, Madalina LU ; Zdrojewska, Izabela Anna and Emnéus, Jenny LU (2006) In Biosensors & Bioelectronics 22(2). p.241-246
Abstract
Progesterone in saliva was monitored using a new method called magnetic particle-based immuno supported liquid membrane assay (m-ISLMA) in a sequential injection (SI) setup, allowing automatic sample cleanup, analyte enrichment, and detection in a single analysis unit. Progesterone (Ag) diffuses from a continuous flowing sample - the donor - into a supported organic liquid membrane (SLM), based on analyte partitioning (solubility) between the aqueous donor and the organic phase. The Ag is re-extracted from the SLM into a second stagnant aqueous acceptor, containing antibodies (Ab) immobilized on magnetic beads, held at the bottom of the acceptor by a magnet. Due to the formation of strong Ag-Ab-bead complexes and a large excess of... (More)
Progesterone in saliva was monitored using a new method called magnetic particle-based immuno supported liquid membrane assay (m-ISLMA) in a sequential injection (SI) setup, allowing automatic sample cleanup, analyte enrichment, and detection in a single analysis unit. Progesterone (Ag) diffuses from a continuous flowing sample - the donor - into a supported organic liquid membrane (SLM), based on analyte partitioning (solubility) between the aqueous donor and the organic phase. The Ag is re-extracted from the SLM into a second stagnant aqueous acceptor, containing antibodies (Ab) immobilized on magnetic beads, held at the bottom of the acceptor by a magnet. Due to the formation of strong Ag-Ab-bead complexes and a large excess of Ab-beads, the Ag is accumulated and selectively enriched in the acceptor. The extracted progesterone was quantified by injecting into the acceptor a horseradish peroxidase (HRP) labeled analyte tracer, the substrate (luminol, H2O2, and p-iodophenol), and finally detection of the generated chemiluminescence by a photomultiplier tube. After optimization of experimental parameters (e.g., sample flow rate, extraction time, type of organic solvent and antibody-bead concentration in the acceptor), a detection limit of 8.50 +/- 0.17 fg L-1 and a dynamic range between 35 fg L-1 and 10 pg L-1 was reached. The progesterone level of saliva for three subjects (women in different period of ovarian cycle) was investigated, and the corresponding progesterone concentrations detected with m-ISLMA coincided well with the expected values. (c) 2006 Elsevier B.V. All rights reserved. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
magnetic particle-based immuno supported liquid membrane assay, (m-ISLMA), saliva, progesterone
in
Biosensors & Bioelectronics
volume
22
issue
2
pages
241 - 246
publisher
Elsevier
external identifiers
  • wos:000240906100012
  • scopus:33747334644
  • pmid:16473507
ISSN
1873-4235
DOI
10.1016/j.bios.2006.01.002
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
id
adb67960-e0b1-449d-b05e-375bb159655a (old id 389767)
date added to LUP
2016-04-01 16:31:38
date last changed
2021-08-04 03:10:26
@article{adb67960-e0b1-449d-b05e-375bb159655a,
  abstract     = {Progesterone in saliva was monitored using a new method called magnetic particle-based immuno supported liquid membrane assay (m-ISLMA) in a sequential injection (SI) setup, allowing automatic sample cleanup, analyte enrichment, and detection in a single analysis unit. Progesterone (Ag) diffuses from a continuous flowing sample - the donor - into a supported organic liquid membrane (SLM), based on analyte partitioning (solubility) between the aqueous donor and the organic phase. The Ag is re-extracted from the SLM into a second stagnant aqueous acceptor, containing antibodies (Ab) immobilized on magnetic beads, held at the bottom of the acceptor by a magnet. Due to the formation of strong Ag-Ab-bead complexes and a large excess of Ab-beads, the Ag is accumulated and selectively enriched in the acceptor. The extracted progesterone was quantified by injecting into the acceptor a horseradish peroxidase (HRP) labeled analyte tracer, the substrate (luminol, H2O2, and p-iodophenol), and finally detection of the generated chemiluminescence by a photomultiplier tube. After optimization of experimental parameters (e.g., sample flow rate, extraction time, type of organic solvent and antibody-bead concentration in the acceptor), a detection limit of 8.50 +/- 0.17 fg L-1 and a dynamic range between 35 fg L-1 and 10 pg L-1 was reached. The progesterone level of saliva for three subjects (women in different period of ovarian cycle) was investigated, and the corresponding progesterone concentrations detected with m-ISLMA coincided well with the expected values. (c) 2006 Elsevier B.V. All rights reserved.},
  author       = {Tudorache, Madalina and Zdrojewska, Izabela Anna and Emnéus, Jenny},
  issn         = {1873-4235},
  language     = {eng},
  number       = {2},
  pages        = {241--246},
  publisher    = {Elsevier},
  series       = {Biosensors & Bioelectronics},
  title        = {Evaluation of progesterone content in saliva using magnetic particle-based immuno supported liquid membrane assay (m-ISLMA)},
  url          = {http://dx.doi.org/10.1016/j.bios.2006.01.002},
  doi          = {10.1016/j.bios.2006.01.002},
  volume       = {22},
  year         = {2006},
}