QseC controls biofilm formation of non-typeable Haemophilus influenzae in addition to an AI-2-dependent mechanism.
Ünal, Can LU ; Singh, Birendra LU ; Fleury, Christophe LU ; Singh, Kalpana LU ; Chávez de Paz, Luis ; Svensäter, Gunnel and Riesbeck, Kristian LU
(2012)
In International Journal of Medical Microbiology
302(6).
p.261-269
- Abstract
- Non-typeable Haemophilus influenzae (NTHi) is a common pathogen associated with diseases such as acute otitis media or exacerbations in patients with chronic obstructive pulmonary disease. The biofilm-forming capability substantially contributes to the persistence of NTHi. However, the regulation of biofilm formation is not completely understood. Quorum sensing regulated by autoinducer-2 produced by luxS is until now the only described regulatory mechanism. In this study, we show that the two-component signalling system QseB/C is involved in the biofilm formation of NTHi in vitro. An isogenic NTHi mutant of qseC (Hi3655KR2) showed a significant decrease in biofilm formation under static and semi-static conditions as assessed by crystal... (More)
- Non-typeable Haemophilus influenzae (NTHi) is a common pathogen associated with diseases such as acute otitis media or exacerbations in patients with chronic obstructive pulmonary disease. The biofilm-forming capability substantially contributes to the persistence of NTHi. However, the regulation of biofilm formation is not completely understood. Quorum sensing regulated by autoinducer-2 produced by luxS is until now the only described regulatory mechanism. In this study, we show that the two-component signalling system QseB/C is involved in the biofilm formation of NTHi in vitro. An isogenic NTHi mutant of qseC (Hi3655KR2) showed a significant decrease in biofilm formation under static and semi-static conditions as assessed by crystal violet staining. In addition, under constant flow conditions, Hi3655KR2 formed less biofilm after 48h. The biofilm defects were irrespective of autoinducer-2 levels. Hence, here we suggest for the first time a regulatory circuit in NTHi, which controls biofilm formation by mechanisms other than or in addition to luxS-dependent factors. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3124231
- author
- Ünal, Can
LU
; Singh, Birendra
LU
; Fleury, Christophe
LU
; Singh, Kalpana
LU
; Chávez de Paz, Luis
; Svensäter, Gunnel
and Riesbeck, Kristian
LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- in
- International Journal of Medical Microbiology
- volume
- 302
- issue
- 6
- pages
- 261 - 269
- publisher
- Elsevier
- external identifiers
-
- wos:000312434400005
- pmid:22954413
- scopus:84867858099
- pmid:22954413
- ISSN
- 1618-0607
- DOI
- 10.1016/j.ijmm.2012.07.013
- language
- English
- LU publication?
- yes
- id
- 390ae65f-b6e6-4c1e-bb44-a057dccb6b20 (old id 3124231)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/22954413?dopt=Abstract
- date added to LUP
- 2016-04-01 10:16:44
- date last changed
- 2022-03-27 06:42:11
@article{390ae65f-b6e6-4c1e-bb44-a057dccb6b20,
abstract = {{Non-typeable Haemophilus influenzae (NTHi) is a common pathogen associated with diseases such as acute otitis media or exacerbations in patients with chronic obstructive pulmonary disease. The biofilm-forming capability substantially contributes to the persistence of NTHi. However, the regulation of biofilm formation is not completely understood. Quorum sensing regulated by autoinducer-2 produced by luxS is until now the only described regulatory mechanism. In this study, we show that the two-component signalling system QseB/C is involved in the biofilm formation of NTHi in vitro. An isogenic NTHi mutant of qseC (Hi3655KR2) showed a significant decrease in biofilm formation under static and semi-static conditions as assessed by crystal violet staining. In addition, under constant flow conditions, Hi3655KR2 formed less biofilm after 48h. The biofilm defects were irrespective of autoinducer-2 levels. Hence, here we suggest for the first time a regulatory circuit in NTHi, which controls biofilm formation by mechanisms other than or in addition to luxS-dependent factors.}},
author = {{Ünal, Can and Singh, Birendra and Fleury, Christophe and Singh, Kalpana and Chávez de Paz, Luis and Svensäter, Gunnel and Riesbeck, Kristian}},
issn = {{1618-0607}},
language = {{eng}},
number = {{6}},
pages = {{261--269}},
publisher = {{Elsevier}},
series = {{International Journal of Medical Microbiology}},
title = {{QseC controls biofilm formation of non-typeable Haemophilus influenzae in addition to an AI-2-dependent mechanism.}},
url = {{https://lup.lub.lu.se/search/files/1709638/3630276.pdf}},
doi = {{10.1016/j.ijmm.2012.07.013}},
volume = {{302}},
year = {{2012}},
}