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Diffusion of lysozyme in gels and liquids - A general approach for the determination of diffusion coefficients using holographic laser interferometry

Mattisson, C; Roger, P; Jönsson, Bengt LU ; Axelsson, Anders LU and Zacchi, Guido LU (2000) In Journal of Chromatography. B 743(1-2). p.151-167
Abstract
A study on diffusion measurements of the protein lysozyme in liquids and agarose gels, at different pH and ionic strengths, has been performed using holographic laser interferometry. The measurements showed that the diffusive flux was very dependent on pH and ionic strength when the protein was not at its isoelectric point or when the charge of the lysozyme molecules was not screened by ions in the solution. Evaluation of the experimental data with Fick's law, resulted in diffusion coefficients for lysozyme that are strongly dependent on pH and ionic strength. Evaluation of the experimental data using a more general transport model, based on chemical potential gradients instead of concentration gradients resulted in lysozyme diffusion... (More)
A study on diffusion measurements of the protein lysozyme in liquids and agarose gels, at different pH and ionic strengths, has been performed using holographic laser interferometry. The measurements showed that the diffusive flux was very dependent on pH and ionic strength when the protein was not at its isoelectric point or when the charge of the lysozyme molecules was not screened by ions in the solution. Evaluation of the experimental data with Fick's law, resulted in diffusion coefficients for lysozyme that are strongly dependent on pH and ionic strength. Evaluation of the experimental data using a more general transport model, based on chemical potential gradients instead of concentration gradients resulted in lysozyme diffusion coefficients that are independent of pH and ionic strength. The chemical potential was estimated by using the Poisson-Boltzmann equation. (C) 2000 Elsevier Science B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
diffusion coefficients, holographic laser interferometry, lysozome
in
Journal of Chromatography. B
volume
743
issue
1-2
pages
151 - 167
publisher
Elsevier
external identifiers
  • wos:000088042000018
  • scopus:0034705682
ISSN
1873-376X
DOI
10.1016/S0378-4347(99)00535-6
language
English
LU publication?
yes
id
9f1fa24f-3837-4008-9105-f5f915824dcc (old id 3911339)
date added to LUP
2013-07-01 08:16:10
date last changed
2017-10-08 04:09:17
@article{9f1fa24f-3837-4008-9105-f5f915824dcc,
  abstract     = {A study on diffusion measurements of the protein lysozyme in liquids and agarose gels, at different pH and ionic strengths, has been performed using holographic laser interferometry. The measurements showed that the diffusive flux was very dependent on pH and ionic strength when the protein was not at its isoelectric point or when the charge of the lysozyme molecules was not screened by ions in the solution. Evaluation of the experimental data with Fick's law, resulted in diffusion coefficients for lysozyme that are strongly dependent on pH and ionic strength. Evaluation of the experimental data using a more general transport model, based on chemical potential gradients instead of concentration gradients resulted in lysozyme diffusion coefficients that are independent of pH and ionic strength. The chemical potential was estimated by using the Poisson-Boltzmann equation. (C) 2000 Elsevier Science B.V. All rights reserved.},
  author       = {Mattisson, C and Roger, P and Jönsson, Bengt and Axelsson, Anders and Zacchi, Guido},
  issn         = {1873-376X},
  keyword      = {diffusion coefficients,holographic laser interferometry,lysozome},
  language     = {eng},
  number       = {1-2},
  pages        = {151--167},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography. B},
  title        = {Diffusion of lysozyme in gels and liquids - A general approach for the determination of diffusion coefficients using holographic laser interferometry},
  url          = {http://dx.doi.org/10.1016/S0378-4347(99)00535-6},
  volume       = {743},
  year         = {2000},
}