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Cellulase production by T-reesei

Reczey, K; Szengyel, Z; Eklund, R and Zacchi, Guido LU (1996) In Bioresource Technology 57(1). p.25-30
Abstract
Steam-pretreated willow has been found to be a suitable substrate for ethanol production in Sweden. The production of Trichoderma reesei cellulases on steam-pretreated willow (SPW) of varying cellulose content has been studied. Batch fermentations were performed in shake flasks, and in 4- and 22-l fermenters. SPW was found to be a suitable carbon source for cellulase production. At an initial solids concentration corresponding to 10 g/l cellulose, the yield was 108 filter paper units (FPU)/g cellulose after 3 days in the 22-l fermenter However, the yield was below 100 FPU/g cellulose in the shake flasks and 4-l fermenter The solid residue of enzymatically hydrolysed SPW was found to be nearly as good a substrate as SPW, although the... (More)
Steam-pretreated willow has been found to be a suitable substrate for ethanol production in Sweden. The production of Trichoderma reesei cellulases on steam-pretreated willow (SPW) of varying cellulose content has been studied. Batch fermentations were performed in shake flasks, and in 4- and 22-l fermenters. SPW was found to be a suitable carbon source for cellulase production. At an initial solids concentration corresponding to 10 g/l cellulose, the yield was 108 filter paper units (FPU)/g cellulose after 3 days in the 22-l fermenter However, the yield was below 100 FPU/g cellulose in the shake flasks and 4-l fermenter The solid residue of enzymatically hydrolysed SPW was found to be nearly as good a substrate as SPW, although the cellulose content was as low as 20% in this hydrolysis residue. Using delignified SPW, the yield of cellulases was low, indicating that lignin does not inhibit cellulase production. By applying a pressure of 1.7 bar in the fermenter, the initial fermentation rate was increased. The productivity was increased from 12.2 FPU/l/h to 16.7 FPU/l/h, due to the higher level of dissolved oxygen at the higher pressure. Copyright (C) 1996 Elsevier Science Ltd. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
enzyme production, cellulases, Trichoderma reesei, lignocellulose, steam, pretreatment
in
Bioresource Technology
volume
57
issue
1
pages
25 - 30
publisher
Elsevier
external identifiers
  • wos:A1996VP46600004
  • scopus:0030200061
ISSN
1873-2976
DOI
10.1016/0960-8524(96)00038-7
language
English
LU publication?
yes
id
b1076648-bdd0-49fc-a739-81efb4d9ddd1 (old id 3911525)
date added to LUP
2013-06-28 13:37:33
date last changed
2017-06-04 04:15:21
@article{b1076648-bdd0-49fc-a739-81efb4d9ddd1,
  abstract     = {Steam-pretreated willow has been found to be a suitable substrate for ethanol production in Sweden. The production of Trichoderma reesei cellulases on steam-pretreated willow (SPW) of varying cellulose content has been studied. Batch fermentations were performed in shake flasks, and in 4- and 22-l fermenters. SPW was found to be a suitable carbon source for cellulase production. At an initial solids concentration corresponding to 10 g/l cellulose, the yield was 108 filter paper units (FPU)/g cellulose after 3 days in the 22-l fermenter However, the yield was below 100 FPU/g cellulose in the shake flasks and 4-l fermenter The solid residue of enzymatically hydrolysed SPW was found to be nearly as good a substrate as SPW, although the cellulose content was as low as 20% in this hydrolysis residue. Using delignified SPW, the yield of cellulases was low, indicating that lignin does not inhibit cellulase production. By applying a pressure of 1.7 bar in the fermenter, the initial fermentation rate was increased. The productivity was increased from 12.2 FPU/l/h to 16.7 FPU/l/h, due to the higher level of dissolved oxygen at the higher pressure. Copyright (C) 1996 Elsevier Science Ltd.},
  author       = {Reczey, K and Szengyel, Z and Eklund, R and Zacchi, Guido},
  issn         = {1873-2976},
  keyword      = {enzyme production,cellulases,Trichoderma reesei,lignocellulose,steam,pretreatment},
  language     = {eng},
  number       = {1},
  pages        = {25--30},
  publisher    = {Elsevier},
  series       = {Bioresource Technology},
  title        = {Cellulase production by T-reesei},
  url          = {http://dx.doi.org/10.1016/0960-8524(96)00038-7},
  volume       = {57},
  year         = {1996},
}