Advanced

Kinetics of ethanol production by recombinant Escherichia coli KO11

Olsson, L; Hahn-Hägerdal, Bärbel LU and Zacchi, Guido LU (1995) In Biotechnology and Bioengineering 45(4). p.356-365
Abstract
The fermentation kinetics for separate as well as simultaneous glucose and xylose fermentation with recombinant ethanologenic Escherichia coli KO11 are presented. Glucose and xylose were consumed simultaneously and exhibited mutual inhibition. The glucose exhibited 15 times stronger inhibition in xylose fermentation than vice versa. The fermentation of condensate from steam-pretreated willow (Salix) was investigated. The kinetics were studied in detoxified as well as in nondetoxified condensate. The fermentation of the condensate followed two phases: First the glucose and some of the pentoses (xylose in addition to small amounts of arabinose) were fermented simultaneously, and then the remaining part of the pentoses were fermented. The... (More)
The fermentation kinetics for separate as well as simultaneous glucose and xylose fermentation with recombinant ethanologenic Escherichia coli KO11 are presented. Glucose and xylose were consumed simultaneously and exhibited mutual inhibition. The glucose exhibited 15 times stronger inhibition in xylose fermentation than vice versa. The fermentation of condensate from steam-pretreated willow (Salix) was investigated. The kinetics were studied in detoxified as well as in nondetoxified condensate. The fermentation of the condensate followed two phases: First the glucose and some of the pentoses (xylose in addition to small amounts of arabinose) were fermented simultaneously, and then the remaining part of the pentoses were fermented. The rate of the first phase was independent of the detoxification method used, whereas the rate of the second phase was found to be strongly dependent. When the condensate was detoxified with overliming in combination with sulfite, which was the best detoxification method investigated, the sugars in the condensate, 9 g/L, were fermented in 11 h. The same fermentation took 150 h in nondetoxified condensate, The experimental data were used to develop an empirical model, describing the batch fermentation of recombinant E. coli KO11 in the condensate. The model is based on Monod kinetics including substrate and product inhibition and the sum of the inhibition exerted by the rest of the inhibitors, lumped together. (C) 1995 John Wiley and Sons, Inc. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
LIGNOCELLULOSIC HYDROLYSATE, KINETIC MODEL, ESCHERICHIA COLI K011, ETHANOL PRODUCTION, FERMENTATION, MIXED SUGAR
in
Biotechnology and Bioengineering
volume
45
issue
4
pages
356 - 365
publisher
John Wiley & Sons
external identifiers
  • wos:A1995QJ67000009
  • scopus:0029239619
ISSN
1097-0290
DOI
10.1002/bit.260450410
language
English
LU publication?
yes
id
5beb75ff-dfe9-4315-aa21-90cb4283fc1b (old id 3911553)
date added to LUP
2013-06-28 12:28:30
date last changed
2017-07-23 03:37:39
@article{5beb75ff-dfe9-4315-aa21-90cb4283fc1b,
  abstract     = {The fermentation kinetics for separate as well as simultaneous glucose and xylose fermentation with recombinant ethanologenic Escherichia coli KO11 are presented. Glucose and xylose were consumed simultaneously and exhibited mutual inhibition. The glucose exhibited 15 times stronger inhibition in xylose fermentation than vice versa. The fermentation of condensate from steam-pretreated willow (Salix) was investigated. The kinetics were studied in detoxified as well as in nondetoxified condensate. The fermentation of the condensate followed two phases: First the glucose and some of the pentoses (xylose in addition to small amounts of arabinose) were fermented simultaneously, and then the remaining part of the pentoses were fermented. The rate of the first phase was independent of the detoxification method used, whereas the rate of the second phase was found to be strongly dependent. When the condensate was detoxified with overliming in combination with sulfite, which was the best detoxification method investigated, the sugars in the condensate, 9 g/L, were fermented in 11 h. The same fermentation took 150 h in nondetoxified condensate, The experimental data were used to develop an empirical model, describing the batch fermentation of recombinant E. coli KO11 in the condensate. The model is based on Monod kinetics including substrate and product inhibition and the sum of the inhibition exerted by the rest of the inhibitors, lumped together. (C) 1995 John Wiley and Sons, Inc.},
  author       = {Olsson, L and Hahn-Hägerdal, Bärbel and Zacchi, Guido},
  issn         = {1097-0290},
  keyword      = {LIGNOCELLULOSIC HYDROLYSATE,KINETIC MODEL,ESCHERICHIA COLI K011,ETHANOL PRODUCTION,FERMENTATION,MIXED SUGAR},
  language     = {eng},
  number       = {4},
  pages        = {356--365},
  publisher    = {John Wiley & Sons},
  series       = {Biotechnology and Bioengineering},
  title        = {Kinetics of ethanol production by recombinant Escherichia coli KO11},
  url          = {http://dx.doi.org/10.1002/bit.260450410},
  volume       = {45},
  year         = {1995},
}