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Performance of batch and continuous reactors with co-immobilized yeast and beta-galactosidase

Axelsson, Anders LU ; Nilsson, M; Zacchi, Guido LU and Hahn-Hägerdal, Bärbel LU (1991) In Journal of Chemical Technology and Biotechnology 52(2). p.227-241
Abstract
The anaerobic fermentation of deproteinized whey with beta-galactosidase coimmobilized with Saccharomyces cerevisiae in calcium alginate gel beads for the production of ethanol has been studied in a continuous horizontal packed bed reactor (HPBR). The results are compared with batch experiments in a stirred tank reactor. The immobilized yeast cells are exposed to conditions that vary with time and location in the reactor, making a true steady state impossible. In spite of a very low specific growth rate-of the order of 0.01 h-1 in the first section of the HPBR-the yeast cell growth, accompanied by bead expansion in this section, was high enough to create a cell concentration gradient along the reactor. The continuous reactor is preferable... (More)
The anaerobic fermentation of deproteinized whey with beta-galactosidase coimmobilized with Saccharomyces cerevisiae in calcium alginate gel beads for the production of ethanol has been studied in a continuous horizontal packed bed reactor (HPBR). The results are compared with batch experiments in a stirred tank reactor. The immobilized yeast cells are exposed to conditions that vary with time and location in the reactor, making a true steady state impossible. In spite of a very low specific growth rate-of the order of 0.01 h-1 in the first section of the HPBR-the yeast cell growth, accompanied by bead expansion in this section, was high enough to create a cell concentration gradient along the reactor. The continuous reactor is preferable to the batch reactor as the galactose conversion is more efficient. The highest volumetric productivity obtained in the HPBR was 125 mol ethanol m-3 h-1 (6 g ethanol dm-3 h-1) at a substrate concentration of 164 mol m-3 lactose (56 g dm-3) and a dilution rate of 0.21 h-1, corresponding to a space velocity of 0.51 dm3 dm-3 gel h-1. The ethanol yield from consumed glucose and galactose was 80%. The ethanol yield from lactose was only 70%, as only 75% of the galactose was consumed while all the lactose and glucose were converted. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
PACKED-BED BIOREACTOR, COIMMOBILIZATION, YEAST, BETA-GALACTOSIDASE, WHEY, ETHANOL, ALGINATE, CELL GROWTH
in
Journal of Chemical Technology and Biotechnology
volume
52
issue
2
pages
227 - 241
publisher
Wiley-Blackwell
external identifiers
  • wos:A1991GK99700009
  • scopus:0025821032
ISSN
0268-2575
DOI
10.1002/jctb.280520210
language
English
LU publication?
yes
id
5a2616ea-8c6c-4969-8230-6273fb67eb97 (old id 3911598)
date added to LUP
2013-06-28 10:41:28
date last changed
2017-03-26 04:13:59
@article{5a2616ea-8c6c-4969-8230-6273fb67eb97,
  abstract     = {The anaerobic fermentation of deproteinized whey with beta-galactosidase coimmobilized with Saccharomyces cerevisiae in calcium alginate gel beads for the production of ethanol has been studied in a continuous horizontal packed bed reactor (HPBR). The results are compared with batch experiments in a stirred tank reactor. The immobilized yeast cells are exposed to conditions that vary with time and location in the reactor, making a true steady state impossible. In spite of a very low specific growth rate-of the order of 0.01 h-1 in the first section of the HPBR-the yeast cell growth, accompanied by bead expansion in this section, was high enough to create a cell concentration gradient along the reactor. The continuous reactor is preferable to the batch reactor as the galactose conversion is more efficient. The highest volumetric productivity obtained in the HPBR was 125 mol ethanol m-3 h-1 (6 g ethanol dm-3 h-1) at a substrate concentration of 164 mol m-3 lactose (56 g dm-3) and a dilution rate of 0.21 h-1, corresponding to a space velocity of 0.51 dm3 dm-3 gel h-1. The ethanol yield from consumed glucose and galactose was 80%. The ethanol yield from lactose was only 70%, as only 75% of the galactose was consumed while all the lactose and glucose were converted.},
  author       = {Axelsson, Anders and Nilsson, M and Zacchi, Guido and Hahn-Hägerdal, Bärbel},
  issn         = {0268-2575},
  keyword      = {PACKED-BED BIOREACTOR,COIMMOBILIZATION,YEAST,BETA-GALACTOSIDASE,WHEY,ETHANOL,ALGINATE,CELL GROWTH},
  language     = {eng},
  number       = {2},
  pages        = {227--241},
  publisher    = {Wiley-Blackwell},
  series       = {Journal of Chemical Technology and Biotechnology},
  title        = {Performance of batch and continuous reactors with co-immobilized yeast and beta-galactosidase},
  url          = {http://dx.doi.org/10.1002/jctb.280520210},
  volume       = {52},
  year         = {1991},
}