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Skin Membrane Electrical Impedance Properties under the Influence of a Varying Water Gradient.

Björklund, Sebastian LU ; Ruzgas, Tautgirdas LU ; Nowacka, Agnieszka LU ; Dahi, Ihab LU ; Topgaard, Daniel LU ; Sparr, Emma LU and Engblom, Johan (2013) In Biophysical Journal 104(12). p.2639-2650
Abstract
The stratum corneum (SC) is an effective permeability barrier. One strategy to increase drug delivery across skin is to increase the hydration. A detailed description of how hydration affects skin permeability requires characterization of both macroscopic and molecular properties and how they respond to hydration. We explore this issue by performing impedance experiments on excised skin membranes in the frequency range 1 Hz to 0.2 MHz under the influence of a varying gradient in water activity (aw). Hydration/dehydration induces reversible changes of membrane resistance and effective capacitance. On average, the membrane resistance is 14 times lower and the effective capacitance is 1.5 times higher when the outermost SC membrane is exposed... (More)
The stratum corneum (SC) is an effective permeability barrier. One strategy to increase drug delivery across skin is to increase the hydration. A detailed description of how hydration affects skin permeability requires characterization of both macroscopic and molecular properties and how they respond to hydration. We explore this issue by performing impedance experiments on excised skin membranes in the frequency range 1 Hz to 0.2 MHz under the influence of a varying gradient in water activity (aw). Hydration/dehydration induces reversible changes of membrane resistance and effective capacitance. On average, the membrane resistance is 14 times lower and the effective capacitance is 1.5 times higher when the outermost SC membrane is exposed to hydrating conditions (aw = 0.992), as compared to the case of more dehydrating conditions (aw = 0.826). Molecular insight into the hydration effects on the SC components is provided by natural-abundance (13)C polarization transfer solid-state NMR and x-ray diffraction under similar hydration conditions. Hydration has a significant effect on the dynamics of the keratin filament terminals and increases the interchain spacing of the filaments. The SC lipids are organized into lamellar structures with ∼ 12.6 nm spacing and hexagonal hydrocarbon chain packing with mainly all-trans configuration of the acyl chains, irrespective of hydration state. Subtle changes in the dynamics of the lipids due to mobilization and incorporation of cholesterol and long-chain lipid species into the fluid lipid fraction is suggested to occur upon hydration, which can explain the changes of the impedance response. The results presented here provide information that is useful in explaining the effect of hydration on skin permeability. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biophysical Journal
volume
104
issue
12
pages
2639 - 2650
publisher
Cell Press
external identifiers
  • wos:000320757100009
  • pmid:23790372
  • scopus:84879202093
ISSN
1542-0086
DOI
10.1016/j.bpj.2013.05.008
language
English
LU publication?
yes
id
85150266-2ccb-4f04-876f-24e7e40e5393 (old id 3913067)
date added to LUP
2013-07-11 10:43:44
date last changed
2019-09-29 03:02:08
@article{85150266-2ccb-4f04-876f-24e7e40e5393,
  abstract     = {The stratum corneum (SC) is an effective permeability barrier. One strategy to increase drug delivery across skin is to increase the hydration. A detailed description of how hydration affects skin permeability requires characterization of both macroscopic and molecular properties and how they respond to hydration. We explore this issue by performing impedance experiments on excised skin membranes in the frequency range 1 Hz to 0.2 MHz under the influence of a varying gradient in water activity (aw). Hydration/dehydration induces reversible changes of membrane resistance and effective capacitance. On average, the membrane resistance is 14 times lower and the effective capacitance is 1.5 times higher when the outermost SC membrane is exposed to hydrating conditions (aw = 0.992), as compared to the case of more dehydrating conditions (aw = 0.826). Molecular insight into the hydration effects on the SC components is provided by natural-abundance (13)C polarization transfer solid-state NMR and x-ray diffraction under similar hydration conditions. Hydration has a significant effect on the dynamics of the keratin filament terminals and increases the interchain spacing of the filaments. The SC lipids are organized into lamellar structures with ∼ 12.6 nm spacing and hexagonal hydrocarbon chain packing with mainly all-trans configuration of the acyl chains, irrespective of hydration state. Subtle changes in the dynamics of the lipids due to mobilization and incorporation of cholesterol and long-chain lipid species into the fluid lipid fraction is suggested to occur upon hydration, which can explain the changes of the impedance response. The results presented here provide information that is useful in explaining the effect of hydration on skin permeability.},
  author       = {Björklund, Sebastian and Ruzgas, Tautgirdas and Nowacka, Agnieszka and Dahi, Ihab and Topgaard, Daniel and Sparr, Emma and Engblom, Johan},
  issn         = {1542-0086},
  language     = {eng},
  number       = {12},
  pages        = {2639--2650},
  publisher    = {Cell Press},
  series       = {Biophysical Journal},
  title        = {Skin Membrane Electrical Impedance Properties under the Influence of a Varying Water Gradient.},
  url          = {http://dx.doi.org/10.1016/j.bpj.2013.05.008},
  volume       = {104},
  year         = {2013},
}