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Cell harvesting by cross-flow microfiltration using a shear-enhanced module

Frenander, U and Jönsson, Ann-Sofi LU (1996) In Biotechnology and Bioengineering 52(3). p.397-403
Abstract
Protein, produced by a bacterial culture of recombinant Vibrio cholerae, was separated from cells in a fermentation broth by cross-flow microfiltration. A new, mechanically agitated (rotational) shear filter, the DMF(TM) filter from Pall, was used to perform the separation. Higher protein recovery and permeate flux than commonly obtained during cell harvesting were demonstrated using sixfold concentration followed by twofold diafiltration. The transmembrane pressure only increased by 10 kPa when the flux was kept constant at 150 L/m(2) h during both concentration and diafiltration. The protein transmission was about 100% initially, and over 90% at the end of the concentration process. The protein transmission during the diafiltration was... (More)
Protein, produced by a bacterial culture of recombinant Vibrio cholerae, was separated from cells in a fermentation broth by cross-flow microfiltration. A new, mechanically agitated (rotational) shear filter, the DMF(TM) filter from Pall, was used to perform the separation. Higher protein recovery and permeate flux than commonly obtained during cell harvesting were demonstrated using sixfold concentration followed by twofold diafiltration. The transmembrane pressure only increased by 10 kPa when the flux was kept constant at 150 L/m(2) h during both concentration and diafiltration. The protein transmission was about 100% initially, and over 90% at the end of the concentration process. The protein transmission during the diafiltration was over 80%. The total recovery of protein was 97%. When using an enzymatic cleaning agent, no significant pure water flux decrease was detected during the course of the experiments. (C) 1996 John Wiley & Sons, Inc. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cell harvesting, protein separation, Vibrio cholerae, cross-flow, microfiltration, shear-enhanced module
in
Biotechnology and Bioengineering
volume
52
issue
3
pages
397 - 403
publisher
John Wiley & Sons
external identifiers
  • wos:A1996VM10500005
  • scopus:0030571269
ISSN
1097-0290
DOI
10.1002/(SICI)1097-0290(19961105)52:3<397::AID-BIT5>3.3.CO;2-1
language
English
LU publication?
yes
id
ae5ec9d8-fe45-4435-bc53-10d6e39ac887 (old id 3915742)
date added to LUP
2013-07-02 11:00:36
date last changed
2017-09-24 03:42:51
@article{ae5ec9d8-fe45-4435-bc53-10d6e39ac887,
  abstract     = {Protein, produced by a bacterial culture of recombinant Vibrio cholerae, was separated from cells in a fermentation broth by cross-flow microfiltration. A new, mechanically agitated (rotational) shear filter, the DMF(TM) filter from Pall, was used to perform the separation. Higher protein recovery and permeate flux than commonly obtained during cell harvesting were demonstrated using sixfold concentration followed by twofold diafiltration. The transmembrane pressure only increased by 10 kPa when the flux was kept constant at 150 L/m(2) h during both concentration and diafiltration. The protein transmission was about 100% initially, and over 90% at the end of the concentration process. The protein transmission during the diafiltration was over 80%. The total recovery of protein was 97%. When using an enzymatic cleaning agent, no significant pure water flux decrease was detected during the course of the experiments. (C) 1996 John Wiley &amp; Sons, Inc.},
  author       = {Frenander, U and Jönsson, Ann-Sofi},
  issn         = {1097-0290},
  keyword      = {cell harvesting,protein separation,Vibrio cholerae,cross-flow,microfiltration,shear-enhanced module},
  language     = {eng},
  number       = {3},
  pages        = {397--403},
  publisher    = {John Wiley & Sons},
  series       = {Biotechnology and Bioengineering},
  title        = {Cell harvesting by cross-flow microfiltration using a shear-enhanced module},
  url          = {http://dx.doi.org/10.1002/(SICI)1097-0290(19961105)52:3<397::AID-BIT5>3.3.CO;2-1},
  volume       = {52},
  year         = {1996},
}