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Visualization of the melanosome transfer-inhibition in a mouse epidermal cell co-culture model.

Kim, Hae Jong; Kazi, Julhash U. LU ; Lee, You Ree; Nguyen, Dung H.; Lee, Hyang Bok; Shin, Jeong Hyun; Soh, Jae Won and Kim, Eun Ki (2010) In Int J Mol Med. 25(2)(Feb). p.249-253
Abstract
Transfer of melanin-containing melanosomes from melanocytes to neighboring keratinocytes results in skin pigmentation. To provide a more practical method of visualizing melanosomes in melanocytes as well as in keratinocytes, we attempted to use murine cell lines instead of human primary cells. We generated various fluorescent fusion proteins of tyrosinase, a melanin synthesis enzyme located in the melanosome, by using green fluorescent protein and red fluorescent protein. The intracellular localization of tyrosinase was then examined by fluorescence and confocal microscopy. Co-culture of murine melanocytes and keratinocytes was optimized and melanosome transfer was either stimulated with alphaMSH or partially inhibited by niacinamide. To... (More)
Transfer of melanin-containing melanosomes from melanocytes to neighboring keratinocytes results in skin pigmentation. To provide a more practical method of visualizing melanosomes in melanocytes as well as in keratinocytes, we attempted to use murine cell lines instead of human primary cells. We generated various fluorescent fusion proteins of tyrosinase, a melanin synthesis enzyme located in the melanosome, by using green fluorescent protein and red fluorescent protein. The intracellular localization of tyrosinase was then examined by fluorescence and confocal microscopy. Co-culture of murine melanocytes and keratinocytes was optimized and melanosome transfer was either stimulated with alphaMSH or partially inhibited by niacinamide. To the best of our knowledge, this is the first study showing that a murine co-culture model, in addition to human primary cell co-culture, can be a good tool for depigmenting agent screening by monitoring melanosome transfer. (Less)
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in
Int J Mol Med.
volume
25(2)
issue
Feb
pages
249 - 253
language
English
LU publication?
no
id
75e3e526-25ff-4e49-87ed-0e5e719f11cb (old id 3915823)
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http://www.ncbi.nlm.nih.gov/pubmed/20043134?dopt=AbstractPlus
date added to LUP
2013-07-02 11:19:11
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2017-02-19 14:41:16
@article{75e3e526-25ff-4e49-87ed-0e5e719f11cb,
  abstract     = {Transfer of melanin-containing melanosomes from melanocytes to neighboring keratinocytes results in skin pigmentation. To provide a more practical method of visualizing melanosomes in melanocytes as well as in keratinocytes, we attempted to use murine cell lines instead of human primary cells. We generated various fluorescent fusion proteins of tyrosinase, a melanin synthesis enzyme located in the melanosome, by using green fluorescent protein and red fluorescent protein. The intracellular localization of tyrosinase was then examined by fluorescence and confocal microscopy. Co-culture of murine melanocytes and keratinocytes was optimized and melanosome transfer was either stimulated with alphaMSH or partially inhibited by niacinamide. To the best of our knowledge, this is the first study showing that a murine co-culture model, in addition to human primary cell co-culture, can be a good tool for depigmenting agent screening by monitoring melanosome transfer.},
  author       = {Kim, Hae Jong and Kazi, Julhash U. and Lee, You Ree and Nguyen, Dung H. and Lee, Hyang Bok and Shin, Jeong Hyun and Soh, Jae Won and Kim, Eun Ki},
  language     = {eng},
  number       = {Feb},
  pages        = {249--253},
  series       = {Int J Mol Med.},
  title        = {Visualization of the melanosome transfer-inhibition in a mouse epidermal cell co-culture model.},
  volume       = {25(2)},
  year         = {2010},
}