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Cost Analysis of Ethanol Production from Willow Using Recombinant Escherichia coli

von Sivers, M; Zacchi, Guido LU ; Olsson, L and Hahn-Hägerdal, Bärbel LU (1994) In Biotechnology Progress 10(5). p.555-560
Abstract
This study comprises a technical and economic analysis of the production of fuel ethanol by fermentation of a pentose-rich hydrolysate with recombinant Escherichia coli, strain KO11. Hydrolysate from steam-pretreated willow was used as raw material in calculations regarding the fermentation. The calculations were based on a feed capacity of 10 metric tons of dry willow per hour to the pretreatment stage, providing 35 metric tons of hydrolysate per hour, consisting of 45 g of sugars/L, to the pentose fermentation plant. A detoxification step was included, since the hydrolysate has been shown to have an inhibitory effect on the E. coli KO11. The technical data used in the calculations were based on a kinetic fermentation model, which was... (More)
This study comprises a technical and economic analysis of the production of fuel ethanol by fermentation of a pentose-rich hydrolysate with recombinant Escherichia coli, strain KO11. Hydrolysate from steam-pretreated willow was used as raw material in calculations regarding the fermentation. The calculations were based on a feed capacity of 10 metric tons of dry willow per hour to the pretreatment stage, providing 35 metric tons of hydrolysate per hour, consisting of 45 g of sugars/L, to the pentose fermentation plant. A detoxification step was included, since the hydrolysate has been shown to have an inhibitory effect on the E. coli KO11. The technical data used in the calculations were based on a kinetic fermentation model, which was developed from laboratory-scale experiments in a previous study. The economic analysis predicted an ethanol production cast of 48 cent/L in the pentose fermentation plant, indicating potentially good economy. The detoxification cost constitutes 22% of this cost. Sensitivity analyses revealed that if the concentration of sugars in the feed to the fermentation was decreased by 40% to 27 g/L, the ethanol production cost was increased to 54 cent/L. The production cost was increased to 50 cent/L ethanol if the cell mass was recirculated to the fermentation stage 5 times instead of 20. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biotechnology Progress
volume
10
issue
5
pages
555 - 560
publisher
The American Chemical Society
external identifiers
  • wos:A1994PL09100017
  • scopus:0028500159
ISSN
1520-6033
DOI
10.1021/bp00029a017
language
English
LU publication?
yes
id
360eb4bc-1799-4c5e-874d-e4f26a8b95df (old id 3918208)
date added to LUP
2013-07-05 09:50:28
date last changed
2017-09-24 04:20:31
@article{360eb4bc-1799-4c5e-874d-e4f26a8b95df,
  abstract     = {This study comprises a technical and economic analysis of the production of fuel ethanol by fermentation of a pentose-rich hydrolysate with recombinant Escherichia coli, strain KO11. Hydrolysate from steam-pretreated willow was used as raw material in calculations regarding the fermentation. The calculations were based on a feed capacity of 10 metric tons of dry willow per hour to the pretreatment stage, providing 35 metric tons of hydrolysate per hour, consisting of 45 g of sugars/L, to the pentose fermentation plant. A detoxification step was included, since the hydrolysate has been shown to have an inhibitory effect on the E. coli KO11. The technical data used in the calculations were based on a kinetic fermentation model, which was developed from laboratory-scale experiments in a previous study. The economic analysis predicted an ethanol production cast of 48 cent/L in the pentose fermentation plant, indicating potentially good economy. The detoxification cost constitutes 22% of this cost. Sensitivity analyses revealed that if the concentration of sugars in the feed to the fermentation was decreased by 40% to 27 g/L, the ethanol production cost was increased to 54 cent/L. The production cost was increased to 50 cent/L ethanol if the cell mass was recirculated to the fermentation stage 5 times instead of 20.},
  author       = {von Sivers, M and Zacchi, Guido and Olsson, L and Hahn-Hägerdal, Bärbel},
  issn         = {1520-6033},
  language     = {eng},
  number       = {5},
  pages        = {555--560},
  publisher    = {The American Chemical Society},
  series       = {Biotechnology Progress},
  title        = {Cost Analysis of Ethanol Production from Willow Using Recombinant Escherichia coli},
  url          = {http://dx.doi.org/10.1021/bp00029a017},
  volume       = {10},
  year         = {1994},
}