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Cost Analysis of Ethanol Production from Willow Using Recombinant Escherichia coli

von Sivers, M ; Zacchi, Guido LU ; Olsson, L and Hahn-Hägerdal, Bärbel LU (1994) In Biotechnology Progress 10(5). p.555-560
Abstract
This study comprises a technical and economic analysis of the production of fuel ethanol by fermentation of a pentose-rich hydrolysate with recombinant Escherichia coli, strain KO11. Hydrolysate from steam-pretreated willow was used as raw material in calculations regarding the fermentation. The calculations were based on a feed capacity of 10 metric tons of dry willow per hour to the pretreatment stage, providing 35 metric tons of hydrolysate per hour, consisting of 45 g of sugars/L, to the pentose fermentation plant. A detoxification step was included, since the hydrolysate has been shown to have an inhibitory effect on the E. coli KO11. The technical data used in the calculations were based on a kinetic fermentation model, which was... (More)
This study comprises a technical and economic analysis of the production of fuel ethanol by fermentation of a pentose-rich hydrolysate with recombinant Escherichia coli, strain KO11. Hydrolysate from steam-pretreated willow was used as raw material in calculations regarding the fermentation. The calculations were based on a feed capacity of 10 metric tons of dry willow per hour to the pretreatment stage, providing 35 metric tons of hydrolysate per hour, consisting of 45 g of sugars/L, to the pentose fermentation plant. A detoxification step was included, since the hydrolysate has been shown to have an inhibitory effect on the E. coli KO11. The technical data used in the calculations were based on a kinetic fermentation model, which was developed from laboratory-scale experiments in a previous study. The economic analysis predicted an ethanol production cast of 48 cent/L in the pentose fermentation plant, indicating potentially good economy. The detoxification cost constitutes 22% of this cost. Sensitivity analyses revealed that if the concentration of sugars in the feed to the fermentation was decreased by 40% to 27 g/L, the ethanol production cost was increased to 54 cent/L. The production cost was increased to 50 cent/L ethanol if the cell mass was recirculated to the fermentation stage 5 times instead of 20. (Less)
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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biotechnology Progress
volume
10
issue
5
pages
555 - 560
publisher
The American Chemical Society (ACS)
external identifiers
  • wos:A1994PL09100017
  • scopus:0028500159
ISSN
1520-6033
DOI
10.1021/bp00029a017
language
English
LU publication?
yes
id
360eb4bc-1799-4c5e-874d-e4f26a8b95df (old id 3918208)
date added to LUP
2016-04-01 15:48:32
date last changed
2021-06-13 04:48:29
@article{360eb4bc-1799-4c5e-874d-e4f26a8b95df,
  abstract     = {{This study comprises a technical and economic analysis of the production of fuel ethanol by fermentation of a pentose-rich hydrolysate with recombinant Escherichia coli, strain KO11. Hydrolysate from steam-pretreated willow was used as raw material in calculations regarding the fermentation. The calculations were based on a feed capacity of 10 metric tons of dry willow per hour to the pretreatment stage, providing 35 metric tons of hydrolysate per hour, consisting of 45 g of sugars/L, to the pentose fermentation plant. A detoxification step was included, since the hydrolysate has been shown to have an inhibitory effect on the E. coli KO11. The technical data used in the calculations were based on a kinetic fermentation model, which was developed from laboratory-scale experiments in a previous study. The economic analysis predicted an ethanol production cast of 48 cent/L in the pentose fermentation plant, indicating potentially good economy. The detoxification cost constitutes 22% of this cost. Sensitivity analyses revealed that if the concentration of sugars in the feed to the fermentation was decreased by 40% to 27 g/L, the ethanol production cost was increased to 54 cent/L. The production cost was increased to 50 cent/L ethanol if the cell mass was recirculated to the fermentation stage 5 times instead of 20.}},
  author       = {{von Sivers, M and Zacchi, Guido and Olsson, L and Hahn-Hägerdal, Bärbel}},
  issn         = {{1520-6033}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{555--560}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{Biotechnology Progress}},
  title        = {{Cost Analysis of Ethanol Production from Willow Using Recombinant Escherichia coli}},
  url          = {{http://dx.doi.org/10.1021/bp00029a017}},
  doi          = {{10.1021/bp00029a017}},
  volume       = {{10}},
  year         = {{1994}},
}