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Succession of bacterial biofilm communities following removal of chloramine from a full-scale drinking water distribution system

Rosenqvist, Tage LU ; Danielsson, Mikael LU ; Schleich, Caroline ; Ahlinder, Jon ; Pullerits, Kristjan LU ; Dacklin, Ingrid ; Salomonsson, Emelie N. ; Sundell, David ; Forsman, Mats and Keucken, Alexander LU , et al. (2023) In npj Clean Water 2023(6).
Abstract
Monochloramine is used to regulate microbial regrowth in drinking water distribution systems (DWDS) but produces carcinogenic disinfection byproducts and constitutes a source of energy for nitrifying bacteria. This study followed biofilm-dispersed microbial communities of a full-scale DWDS distributing ultrafiltered water over three years, before and after removal of monochloramine. Communities were described using flow cytometry and amplicon sequencing, including full-length 16S rRNA gene sequencing. Removal of monochloramine increased total cell counts by up to 440%. Increased abundance of heterotrophic bacteria was followed by emergence of the predatory bacteria Bdellovibrio, and a community potentially metabolizing small organic... (More)
Monochloramine is used to regulate microbial regrowth in drinking water distribution systems (DWDS) but produces carcinogenic disinfection byproducts and constitutes a source of energy for nitrifying bacteria. This study followed biofilm-dispersed microbial communities of a full-scale DWDS distributing ultrafiltered water over three years, before and after removal of monochloramine. Communities were described using flow cytometry and amplicon sequencing, including full-length 16S rRNA gene sequencing. Removal of monochloramine increased total cell counts by up to 440%. Increased abundance of heterotrophic bacteria was followed by emergence of the predatory bacteria Bdellovibrio, and a community potentially metabolizing small organic compounds replaced the nitrifying core community. No increased abundance of Mycobacterium or Legionella was observed. Co-occurrence analysis identified a network of Nitrosomonas, Nitrospira, Sphingomonas and Hyphomicrobium, suggesting that monochloramine supported this biofilm community. While some species expanded into the changed niche, no immediate biological risk to consumers was indicated within the DWDS. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
npj Clean Water
volume
2023
issue
6
article number
41
publisher
Springer Nature
external identifiers
  • scopus:85159665716
ISSN
2059-7037
DOI
10.1038/s41545-023-00253-x
language
English
LU publication?
yes
id
39383d8f-f2c9-4434-b141-e4d4c2dcc36b
date added to LUP
2023-05-22 14:42:11
date last changed
2023-07-29 04:01:22
@article{39383d8f-f2c9-4434-b141-e4d4c2dcc36b,
  abstract     = {{Monochloramine is used to regulate microbial regrowth in drinking water distribution systems (DWDS) but produces carcinogenic disinfection byproducts and constitutes a source of energy for nitrifying bacteria. This study followed biofilm-dispersed microbial communities of a full-scale DWDS distributing ultrafiltered water over three years, before and after removal of monochloramine. Communities were described using flow cytometry and amplicon sequencing, including full-length 16S rRNA gene sequencing. Removal of monochloramine increased total cell counts by up to 440%. Increased abundance of heterotrophic bacteria was followed by emergence of the predatory bacteria Bdellovibrio, and a community potentially metabolizing small organic compounds replaced the nitrifying core community. No increased abundance of Mycobacterium or Legionella was observed. Co-occurrence analysis identified a network of Nitrosomonas, Nitrospira, Sphingomonas and Hyphomicrobium, suggesting that monochloramine supported this biofilm community. While some species expanded into the changed niche, no immediate biological risk to consumers was indicated within the DWDS.}},
  author       = {{Rosenqvist, Tage and Danielsson, Mikael and Schleich, Caroline and Ahlinder, Jon and Pullerits, Kristjan and Dacklin, Ingrid and Salomonsson, Emelie N. and Sundell, David and Forsman, Mats and Keucken, Alexander and Rådström, Peter and Paul, Catherine J.}},
  issn         = {{2059-7037}},
  language     = {{eng}},
  month        = {{05}},
  number       = {{6}},
  publisher    = {{Springer Nature}},
  series       = {{npj Clean Water}},
  title        = {{Succession of bacterial biofilm communities following removal of chloramine from a full-scale drinking water distribution system}},
  url          = {{http://dx.doi.org/10.1038/s41545-023-00253-x}},
  doi          = {{10.1038/s41545-023-00253-x}},
  volume       = {{2023}},
  year         = {{2023}},
}