Dynamics of Mitochondrial Proteome and Acetylome in Glioblastoma Cells with Contrasting Metabolic Phenotypes
(2024) In International Journal of Molecular Sciences 25(6).- Abstract
Glioblastoma, a type of cancer affecting the central nervous system, is characterized by its poor prognosis and the dynamic alteration of its metabolic phenotype to fuel development and progression. Critical to cellular metabolism, mitochondria play a pivotal role, where the acetylation of lysine residues on mitochondrial enzymes emerges as a crucial regulatory mechanism of protein function. This post-translational modification, which negatively impacts the mitochondrial proteome’s functionality, is modulated by the enzyme sirtuin 3 (SIRT3). Aiming to elucidate the regulatory role of SIRT3 in mitochondrial metabolism within glioblastoma, we employed high-resolution mass spectrometry to analyze the proteome and acetylome of two... (More)
Glioblastoma, a type of cancer affecting the central nervous system, is characterized by its poor prognosis and the dynamic alteration of its metabolic phenotype to fuel development and progression. Critical to cellular metabolism, mitochondria play a pivotal role, where the acetylation of lysine residues on mitochondrial enzymes emerges as a crucial regulatory mechanism of protein function. This post-translational modification, which negatively impacts the mitochondrial proteome’s functionality, is modulated by the enzyme sirtuin 3 (SIRT3). Aiming to elucidate the regulatory role of SIRT3 in mitochondrial metabolism within glioblastoma, we employed high-resolution mass spectrometry to analyze the proteome and acetylome of two glioblastoma cell lines, each exhibiting distinct metabolic behaviors, following the chemical inhibition of SIRT3. Our findings reveal that the protein synthesis machinery, regulated by lysine acetylation, significantly influences the metabolic phenotype of these cells. Moreover, we have shed light on potential novel SIRT3 targets, thereby unveiling new avenues for future investigations. This research highlights the critical function of SIRT3 in mitochondrial metabolism and its broader implications for cellular energetics. It also provides a comparative analysis of the proteome and acetylome across glioblastoma cell lines with opposing metabolic phenotypes.
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- author
- Fernández-Coto, Diana Lashidua ; Gil, Jeovanis LU ; Ayala, Guadalupe and Encarnación-Guevara, Sergio
- organization
- publishing date
- 2024-03
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- glioblastoma, lysine acetylation, metabolism, mitochondria, SIRT3
- in
- International Journal of Molecular Sciences
- volume
- 25
- issue
- 6
- article number
- 3450
- publisher
- MDPI AG
- external identifiers
-
- pmid:38542426
- scopus:85189106961
- ISSN
- 1661-6596
- DOI
- 10.3390/ijms25063450
- language
- English
- LU publication?
- yes
- id
- 39399c75-4fd3-48af-85a2-2fa337d991b1
- date added to LUP
- 2024-04-18 14:58:41
- date last changed
- 2024-04-19 03:00:03
@article{39399c75-4fd3-48af-85a2-2fa337d991b1,
abstract = {{<p>Glioblastoma, a type of cancer affecting the central nervous system, is characterized by its poor prognosis and the dynamic alteration of its metabolic phenotype to fuel development and progression. Critical to cellular metabolism, mitochondria play a pivotal role, where the acetylation of lysine residues on mitochondrial enzymes emerges as a crucial regulatory mechanism of protein function. This post-translational modification, which negatively impacts the mitochondrial proteome’s functionality, is modulated by the enzyme sirtuin 3 (SIRT3). Aiming to elucidate the regulatory role of SIRT3 in mitochondrial metabolism within glioblastoma, we employed high-resolution mass spectrometry to analyze the proteome and acetylome of two glioblastoma cell lines, each exhibiting distinct metabolic behaviors, following the chemical inhibition of SIRT3. Our findings reveal that the protein synthesis machinery, regulated by lysine acetylation, significantly influences the metabolic phenotype of these cells. Moreover, we have shed light on potential novel SIRT3 targets, thereby unveiling new avenues for future investigations. This research highlights the critical function of SIRT3 in mitochondrial metabolism and its broader implications for cellular energetics. It also provides a comparative analysis of the proteome and acetylome across glioblastoma cell lines with opposing metabolic phenotypes.</p>}},
author = {{Fernández-Coto, Diana Lashidua and Gil, Jeovanis and Ayala, Guadalupe and Encarnación-Guevara, Sergio}},
issn = {{1661-6596}},
keywords = {{glioblastoma; lysine acetylation; metabolism; mitochondria; SIRT3}},
language = {{eng}},
number = {{6}},
publisher = {{MDPI AG}},
series = {{International Journal of Molecular Sciences}},
title = {{Dynamics of Mitochondrial Proteome and Acetylome in Glioblastoma Cells with Contrasting Metabolic Phenotypes}},
url = {{http://dx.doi.org/10.3390/ijms25063450}},
doi = {{10.3390/ijms25063450}},
volume = {{25}},
year = {{2024}},
}