Competition and cooperation between tenascin-R, lecticans and contactin 1 regulate neurite growth and morphology
(2006) In Journal of Cell Science 119(16). p.3456-3466- Abstract
- The extracellular matrix molecule tenascin-R (TN-R) and the proteoglycans of the lectican family show an overlapping distribution in the developing brain, have been implicated in similar cellular processes and form a complex network of interactions. Previously, we have demonstrated that TN-R induces microprocesses along neurites and enlarged growth cones of tectal cells by interacting with the cell adhesion molecule contactin 1. Here, we describe competition and cooperation between TN-R, lecticans and contactin 1, and their functional consequences for tectal cells. Aggrecan, brevican and neurocan inhibit the effects of TN-R on microprocess formation and growth cone size. This blocking effect is due to competition of lecticans with binding... (More)
- The extracellular matrix molecule tenascin-R (TN-R) and the proteoglycans of the lectican family show an overlapping distribution in the developing brain, have been implicated in similar cellular processes and form a complex network of interactions. Previously, we have demonstrated that TN-R induces microprocesses along neurites and enlarged growth cones of tectal cells by interacting with the cell adhesion molecule contactin 1. Here, we describe competition and cooperation between TN-R, lecticans and contactin 1, and their functional consequences for tectal cells. Aggrecan, brevican and neurocan inhibit the effects of TN-R on microprocess formation and growth cone size. This blocking effect is due to competition of lecticans with binding of TN-R to its neuronal receptor contactin 1, as shown by a sandwich-binding assay. Interaction of aggrecan with TN-R fibronectin type III domains 4-A is necessary for its inhibitory effect on both microprocess formation and TN-R binding to contactin 1. However, the chondroitin sulfate chains are not involved. Time-lapse video microscopy showed that aggrecan has no acute effect on motility and morphology of microprocesses and growth cones but induces long-term neurite retraction after pre-treatment with TN-R. In contrast to the competition described above, TN-R cooperates with brevican and neurocan to induce attachment of tectal cells and neurite outgrowth, probably by forming a bridge between the lectican substrate and contactin 1 as the neuronal receptor. Our findings suggest that a complex network of protein-protein interactions within the brain extracellular matrix, as shown here for TN-R and lecticans, is important for the fine-regulation of developmental processes such as microprocess formation along the neurite and neurite outgrowth. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/394111
- author
- Zacharias, Ute and Rauch, Uwe LU
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- chondroitin sulfate proteoglycans, tenascin-R, neuritic, contactin 1, microprocesses, growth cone
- in
- Journal of Cell Science
- volume
- 119
- issue
- 16
- pages
- 3456 - 3466
- publisher
- The Company of Biologists Ltd
- external identifiers
-
- pmid:16899820
- wos:000240381300019
- scopus:33748763563
- ISSN
- 0021-9533
- DOI
- 10.1242/jcs.03094
- language
- English
- LU publication?
- yes
- id
- 9b2de6b3-289b-409c-bded-bd3e47fda1de (old id 394111)
- date added to LUP
- 2016-04-01 11:53:51
- date last changed
- 2022-04-20 23:25:21
@article{9b2de6b3-289b-409c-bded-bd3e47fda1de,
abstract = {{The extracellular matrix molecule tenascin-R (TN-R) and the proteoglycans of the lectican family show an overlapping distribution in the developing brain, have been implicated in similar cellular processes and form a complex network of interactions. Previously, we have demonstrated that TN-R induces microprocesses along neurites and enlarged growth cones of tectal cells by interacting with the cell adhesion molecule contactin 1. Here, we describe competition and cooperation between TN-R, lecticans and contactin 1, and their functional consequences for tectal cells. Aggrecan, brevican and neurocan inhibit the effects of TN-R on microprocess formation and growth cone size. This blocking effect is due to competition of lecticans with binding of TN-R to its neuronal receptor contactin 1, as shown by a sandwich-binding assay. Interaction of aggrecan with TN-R fibronectin type III domains 4-A is necessary for its inhibitory effect on both microprocess formation and TN-R binding to contactin 1. However, the chondroitin sulfate chains are not involved. Time-lapse video microscopy showed that aggrecan has no acute effect on motility and morphology of microprocesses and growth cones but induces long-term neurite retraction after pre-treatment with TN-R. In contrast to the competition described above, TN-R cooperates with brevican and neurocan to induce attachment of tectal cells and neurite outgrowth, probably by forming a bridge between the lectican substrate and contactin 1 as the neuronal receptor. Our findings suggest that a complex network of protein-protein interactions within the brain extracellular matrix, as shown here for TN-R and lecticans, is important for the fine-regulation of developmental processes such as microprocess formation along the neurite and neurite outgrowth.}},
author = {{Zacharias, Ute and Rauch, Uwe}},
issn = {{0021-9533}},
keywords = {{chondroitin sulfate proteoglycans; tenascin-R; neuritic; contactin 1; microprocesses; growth cone}},
language = {{eng}},
number = {{16}},
pages = {{3456--3466}},
publisher = {{The Company of Biologists Ltd}},
series = {{Journal of Cell Science}},
title = {{Competition and cooperation between tenascin-R, lecticans and contactin 1 regulate neurite growth and morphology}},
url = {{http://dx.doi.org/10.1242/jcs.03094}},
doi = {{10.1242/jcs.03094}},
volume = {{119}},
year = {{2006}},
}