Nontypeable Haemophilus influenzae strains with the capsule-associated insertion element IS1016 may mimic encapsulated strains
(2006) In APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 114(9). p.633-640- Abstract
- With the elimination of Haemophilus influenzae type b through vaccination, it has been suggested that other types of H. influenzae strains might acquire virulence traits and emerge as important pathogens. The gene sequence IS1016 has been associated with an increased capacity to cause severe infections. It is usually present in encapsulated strains but is sometimes harbored by nontypeable H. influenzae strains. To explore this further, 118 H. influenzae isolates, collected from both patients and healthy carriers, were investigated with PCR with reference to this gene sequence. Isolates positive for the insertion element were bio- and serotyped. The presence of hmw genes for adherence, the genetic profile, and the ability to form biofilm in... (More)
- With the elimination of Haemophilus influenzae type b through vaccination, it has been suggested that other types of H. influenzae strains might acquire virulence traits and emerge as important pathogens. The gene sequence IS1016 has been associated with an increased capacity to cause severe infections. It is usually present in encapsulated strains but is sometimes harbored by nontypeable H. influenzae strains. To explore this further, 118 H. influenzae isolates, collected from both patients and healthy carriers, were investigated with PCR with reference to this gene sequence. Isolates positive for the insertion element were bio- and serotyped. The presence of hmw genes for adherence, the genetic profile, and the ability to form biofilm in vitro were investigated. A total of 15 isolates were IS1016-positive, whereof 12 were nontypeable. All 12 nontypeable isolates were obtained from healthy carriers, and 92% of the isolates were biotype I. They cross-reacted to some extent with type-specific antisera or exhibited a restricted genetic diversity like encapsulated strains. Furthermore, they lacked hmw-genes, and their ability to form biofilms was comparable with a capsule-deficient type b strain. Although this subset of strains mimicked traits usually exhibited by encapsulated strains, the isolation frequency did not seem to have been affected by vaccination. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/394900
- author
- Karlsson, Eva and Melhus, Åsa LU
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- cross-reaction, nontypeable Haemophilus influenzae, serotype, genes, biofilm, hmw
- in
- APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
- volume
- 114
- issue
- 9
- pages
- 633 - 640
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000240206800006
- pmid:16948816
- scopus:33748349658
- ISSN
- 1600-0463
- DOI
- 10.1111/j.1600-0463.2006.apm_333.x
- language
- English
- LU publication?
- yes
- id
- 74ceba28-affa-401a-8f0d-bce59d8e7802 (old id 394900)
- date added to LUP
- 2016-04-01 12:31:49
- date last changed
- 2022-04-29 07:14:04
@article{74ceba28-affa-401a-8f0d-bce59d8e7802,
abstract = {{With the elimination of Haemophilus influenzae type b through vaccination, it has been suggested that other types of H. influenzae strains might acquire virulence traits and emerge as important pathogens. The gene sequence IS1016 has been associated with an increased capacity to cause severe infections. It is usually present in encapsulated strains but is sometimes harbored by nontypeable H. influenzae strains. To explore this further, 118 H. influenzae isolates, collected from both patients and healthy carriers, were investigated with PCR with reference to this gene sequence. Isolates positive for the insertion element were bio- and serotyped. The presence of hmw genes for adherence, the genetic profile, and the ability to form biofilm in vitro were investigated. A total of 15 isolates were IS1016-positive, whereof 12 were nontypeable. All 12 nontypeable isolates were obtained from healthy carriers, and 92% of the isolates were biotype I. They cross-reacted to some extent with type-specific antisera or exhibited a restricted genetic diversity like encapsulated strains. Furthermore, they lacked hmw-genes, and their ability to form biofilms was comparable with a capsule-deficient type b strain. Although this subset of strains mimicked traits usually exhibited by encapsulated strains, the isolation frequency did not seem to have been affected by vaccination.}},
author = {{Karlsson, Eva and Melhus, Åsa}},
issn = {{1600-0463}},
keywords = {{cross-reaction; nontypeable Haemophilus influenzae; serotype; genes; biofilm; hmw}},
language = {{eng}},
number = {{9}},
pages = {{633--640}},
publisher = {{John Wiley & Sons Inc.}},
series = {{APMIS : acta pathologica, microbiologica, et immunologica Scandinavica}},
title = {{Nontypeable Haemophilus influenzae strains with the capsule-associated insertion element IS1016 may mimic encapsulated strains}},
url = {{http://dx.doi.org/10.1111/j.1600-0463.2006.apm_333.x}},
doi = {{10.1111/j.1600-0463.2006.apm_333.x}},
volume = {{114}},
year = {{2006}},
}