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Production of Trichoderma reesei Cel7B and its catalytic core on glucose medium and its application for the treatment of secondary fibers

Dienes, Dora ; Börjesson, Johan LU ; Stålbrand, Henrik LU and Reczey, Kati (2006) In Process Biochemistry 41(9). p.2092-2096
Abstract
Application of cellulolytic enzymes for upgrading of secondary fibers has extensively been studied during the last decades. In many cases, research has been carried out with industrial enzyme preparations which often consist of several enzyme components. The presence of various enzymes makes the determination of the role of each of them difficult in changes of pulp and paper properties. Therefore, investigations using single enzyme preparations are necessary to understand and optimize this complex process. One of the possibilities to produce individual cellulase components is repression of undesired genes. In this work, two recombinants of Trichoderma reesei strain QM9414 were used to produce intact Ce17B (endoglucanase 1, EGI) and the... (More)
Application of cellulolytic enzymes for upgrading of secondary fibers has extensively been studied during the last decades. In many cases, research has been carried out with industrial enzyme preparations which often consist of several enzyme components. The presence of various enzymes makes the determination of the role of each of them difficult in changes of pulp and paper properties. Therefore, investigations using single enzyme preparations are necessary to understand and optimize this complex process. One of the possibilities to produce individual cellulase components is repression of undesired genes. In this work, two recombinants of Trichoderma reesei strain QM9414 were used to produce intact Ce17B (endoglucanase 1, EGI) and the catalytic core of Ce17B selectively under glucose repression of other cellulases. For homologous expression in glucose medium the gpdA promoter from Aspergillus nidulans was used. Culture filtrates containing the intact Ce17B or Ce17B core were applied for secondary fiber treatment. The treatments caused improved drainage as shown by 16-17% decrease of the Schopper-Riegler (SR) values. The effects of cellulase treatment on pulp and paper properties showed that presence or absence of a cellulose-binding module (CBM) on Ce17B does not play a determinative role. (c) 2006 Elsevier Ltd. All rights reserved. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
properties, strength, drainage, recycled fiber, cellulose-binding module (CBM), biotechnology, Trichoderma reesei Cel7B (endoglucanase I)
in
Process Biochemistry
volume
41
issue
9
pages
2092 - 2096
publisher
Elsevier
external identifiers
  • wos:000239868300027
  • scopus:33746358829
ISSN
1873-3298
DOI
10.1016/j.procbio.2006.04.017
language
English
LU publication?
yes
id
4451d8fc-67f1-441a-9ba7-240381e6ae40 (old id 395452)
date added to LUP
2016-04-01 11:54:16
date last changed
2021-09-22 03:31:06
@article{4451d8fc-67f1-441a-9ba7-240381e6ae40,
  abstract     = {Application of cellulolytic enzymes for upgrading of secondary fibers has extensively been studied during the last decades. In many cases, research has been carried out with industrial enzyme preparations which often consist of several enzyme components. The presence of various enzymes makes the determination of the role of each of them difficult in changes of pulp and paper properties. Therefore, investigations using single enzyme preparations are necessary to understand and optimize this complex process. One of the possibilities to produce individual cellulase components is repression of undesired genes. In this work, two recombinants of Trichoderma reesei strain QM9414 were used to produce intact Ce17B (endoglucanase 1, EGI) and the catalytic core of Ce17B selectively under glucose repression of other cellulases. For homologous expression in glucose medium the gpdA promoter from Aspergillus nidulans was used. Culture filtrates containing the intact Ce17B or Ce17B core were applied for secondary fiber treatment. The treatments caused improved drainage as shown by 16-17% decrease of the Schopper-Riegler (SR) values. The effects of cellulase treatment on pulp and paper properties showed that presence or absence of a cellulose-binding module (CBM) on Ce17B does not play a determinative role. (c) 2006 Elsevier Ltd. All rights reserved.},
  author       = {Dienes, Dora and Börjesson, Johan and Stålbrand, Henrik and Reczey, Kati},
  issn         = {1873-3298},
  language     = {eng},
  number       = {9},
  pages        = {2092--2096},
  publisher    = {Elsevier},
  series       = {Process Biochemistry},
  title        = {Production of Trichoderma reesei Cel7B and its catalytic core on glucose medium and its application for the treatment of secondary fibers},
  url          = {http://dx.doi.org/10.1016/j.procbio.2006.04.017},
  doi          = {10.1016/j.procbio.2006.04.017},
  volume       = {41},
  year         = {2006},
}