The G protein-coupled oestrogen receptor 1 agonist G-1 disrupts endothelial cell microtubule structure in a receptor-independent manner.
Holm, Anders LU ; Grände, Per-Olof LU ; Ludueña, Richard F ; Olde, Björn LU ; Prasad, Veena ; Leeb-Lundberg, Fredrik LU and Nilsson, Bengt-Olof LU
(2012)
In Molecular and Cellular Biochemistry
366(1-2).
p.239-249
- Abstract
- The G protein-coupled oestrogen receptor GPER1, also known as GPR30, has been implicated in oestrogen signalling, but the physiological importance of GPER1 is not fully understood. The GPER1 agonist G-1 has become an important tool to assess GPER1-mediated cellular effects. Here, we report that this substance, besides acting via GPER1, affects the microtubule network in endothelial cells. Treatment with G-1 (3 μM) for 24 h reduced DNA synthesis by about 60 % in mouse microvascular endothelial bEnd.3 cells. Treatment with 3 μM G-1 prevented outgrowth of primary endothelial cells from mouse aortic explants embedded in Matrigel. Treatment with G-1 (0.3-3 μM) for 24 h disrupted bEnd.3 cell and HUVEC microtubule structure in a... (More)
- The G protein-coupled oestrogen receptor GPER1, also known as GPR30, has been implicated in oestrogen signalling, but the physiological importance of GPER1 is not fully understood. The GPER1 agonist G-1 has become an important tool to assess GPER1-mediated cellular effects. Here, we report that this substance, besides acting via GPER1, affects the microtubule network in endothelial cells. Treatment with G-1 (3 μM) for 24 h reduced DNA synthesis by about 60 % in mouse microvascular endothelial bEnd.3 cells. Treatment with 3 μM G-1 prevented outgrowth of primary endothelial cells from mouse aortic explants embedded in Matrigel. Treatment with G-1 (0.3-3 μM) for 24 h disrupted bEnd.3 cell and HUVEC microtubule structure in a concentration-dependent manner as assessed by laser-scanning confocal immunofluorescence microscopy. G-1-induced (3 μM) disruption of microtubule was observed also after acute (3 and 6 h) treatment and in the presence of the protein synthesis inhibitor cycloheximide. Disruption of microtubules by 3 μM G-1 was observed in aortic smooth muscle cells obtained from both GPER1 knockout and wild-type mice, suggesting that G-1 influences microtubules through a mechanism independent of GPER1. G-1 dose dependently (10-50 μM) stimulated microtubule assembly in vitro. On the other hand, microtubules appeared normal in the presence of 10-50 μM G-1 as determined by electron microscopy. We suggest that G-1-promoted endothelial cell anti-proliferation is due in part to alteration of microtubule organization through a mechanism independent of GPER1. This G-1-promoted mechanism may be used to block unwanted endothelial cell proliferation and angiogenesis such as that observed in, e.g. cancer. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2431424
- author
- Holm, Anders
LU
; Grände, Per-Olof
LU
; Ludueña, Richard F
; Olde, Björn
LU
; Prasad, Veena
; Leeb-Lundberg, Fredrik
LU
and Nilsson, Bengt-Olof
LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Molecular and Cellular Biochemistry
- volume
- 366
- issue
- 1-2
- pages
- 239 - 249
- publisher
- Springer
- external identifiers
-
- wos:000304877900025
- pmid:22451019
- scopus:84862019563
- ISSN
- 0300-8177
- DOI
- 10.1007/s11010-012-1301-3
- language
- English
- LU publication?
- yes
- id
- 3965a8a0-d884-4df6-8f8b-104c7b29b865 (old id 2431424)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/22451019?dopt=Abstract
- date added to LUP
- 2016-04-01 14:55:50
- date last changed
- 2022-03-22 02:36:02
@article{3965a8a0-d884-4df6-8f8b-104c7b29b865,
abstract = {{The G protein-coupled oestrogen receptor GPER1, also known as GPR30, has been implicated in oestrogen signalling, but the physiological importance of GPER1 is not fully understood. The GPER1 agonist G-1 has become an important tool to assess GPER1-mediated cellular effects. Here, we report that this substance, besides acting via GPER1, affects the microtubule network in endothelial cells. Treatment with G-1 (3 μM) for 24 h reduced DNA synthesis by about 60 % in mouse microvascular endothelial bEnd.3 cells. Treatment with 3 μM G-1 prevented outgrowth of primary endothelial cells from mouse aortic explants embedded in Matrigel. Treatment with G-1 (0.3-3 μM) for 24 h disrupted bEnd.3 cell and HUVEC microtubule structure in a concentration-dependent manner as assessed by laser-scanning confocal immunofluorescence microscopy. G-1-induced (3 μM) disruption of microtubule was observed also after acute (3 and 6 h) treatment and in the presence of the protein synthesis inhibitor cycloheximide. Disruption of microtubules by 3 μM G-1 was observed in aortic smooth muscle cells obtained from both GPER1 knockout and wild-type mice, suggesting that G-1 influences microtubules through a mechanism independent of GPER1. G-1 dose dependently (10-50 μM) stimulated microtubule assembly in vitro. On the other hand, microtubules appeared normal in the presence of 10-50 μM G-1 as determined by electron microscopy. We suggest that G-1-promoted endothelial cell anti-proliferation is due in part to alteration of microtubule organization through a mechanism independent of GPER1. This G-1-promoted mechanism may be used to block unwanted endothelial cell proliferation and angiogenesis such as that observed in, e.g. cancer.}},
author = {{Holm, Anders and Grände, Per-Olof and Ludueña, Richard F and Olde, Björn and Prasad, Veena and Leeb-Lundberg, Fredrik and Nilsson, Bengt-Olof}},
issn = {{0300-8177}},
language = {{eng}},
number = {{1-2}},
pages = {{239--249}},
publisher = {{Springer}},
series = {{Molecular and Cellular Biochemistry}},
title = {{The G protein-coupled oestrogen receptor 1 agonist G-1 disrupts endothelial cell microtubule structure in a receptor-independent manner.}},
url = {{https://lup.lub.lu.se/search/files/4248905/2861104.pdf}},
doi = {{10.1007/s11010-012-1301-3}},
volume = {{366}},
year = {{2012}},
}