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Histamine uptake by human endometrial cells expressing the organic cation transporter EMT and the vesicular monoamine transporter-2

Casslén, Vera LU ; Bottalico, Barbara ; Olsson, Hanna ; Ehinger, Anna LU ; Pilka, Radovan LU ; Casslén, Bertil LU and Hansson, Stefan LU (2006) In Molecular Human Reproduction 12(8). p.483-489
Abstract
Cellular reuptake of monoamines, which is mediated by cell membrane transporters, is followed by accumulation in vesicles by vesicular monoamine transporters (VMAT). The aim of this study was to demonstrate the presence of functional monoamine transporters with high affinity for histamine in human endometrial tissue, since histamine has been implicated as a paracrine signal during endometrial decidualization and embryo implantation. In situ hybridization with S-35-labelled cRNA probes was used for detection of the organic cationic transporter-2 (OCT-2), the extraneuronal monoamine transporter (EMT), and VMAT-2 in cryosections of normal human endometrial tissue. To identify functional transporters for histamine in endometrial cells, we... (More)
Cellular reuptake of monoamines, which is mediated by cell membrane transporters, is followed by accumulation in vesicles by vesicular monoamine transporters (VMAT). The aim of this study was to demonstrate the presence of functional monoamine transporters with high affinity for histamine in human endometrial tissue, since histamine has been implicated as a paracrine signal during endometrial decidualization and embryo implantation. In situ hybridization with S-35-labelled cRNA probes was used for detection of the organic cationic transporter-2 (OCT-2), the extraneuronal monoamine transporter (EMT), and VMAT-2 in cryosections of normal human endometrial tissue. To identify functional transporters for histamine in endometrial cells, we incubated primary cultures of stromal cells and cultures of attached glands with H-3-labelled histamine. Cultures were pretreated with either corticosterone, a specific inhibitor of EMT, or reserpine, a specific inhibitor of VMAT-2. EMT mRNA was localized in the stroma with peak expression in the secretory phase, whereas OCT-2 mRNA was expressed by few cells in the stroma throughout the cycle. VMAT-2 mRNA was localized in the stroma during the proliferative phase and in the epithelium during the secretory phase. Thus, EMT and VMAT-2, which both have high affinity for histamine, are strongly expressed in endometrial cells. Both corticosterone and reserpine significantly reduced the uptake of H-3-histamine in stromal cells during the proliferative as well as the secretory phase. This indicates the presence of functional EMT and VMAT-2 transporter proteins throughout the cycle, even though their periods of maximal mRNA expression were limited. The results of uptake experiments with glandular epithelial cells confirmed not only the presence of functional VMAT-2 transporter protein in the secretory phase but also the absence of a histamine-specific plasma membrane transporter throughout the cycle. Thus, endometrial tissue contains both plasma membrane and vesicular membrane monoamine transporters with high affinity for histamine. They can potentially influence the reproductive process by the uptake of extracellular histamine and subsequent release on demand. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
decidualization, histamine release, mediator, stromal cell, inflammatory, epithelial cell
in
Molecular Human Reproduction
volume
12
issue
8
pages
483 - 489
publisher
Oxford University Press
external identifiers
  • pmid:16567367
  • wos:000239905500002
  • scopus:33747877256
ISSN
1460-2407
DOI
10.1093/molehr/gah259
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Pathology, (Lund) (013030000), Department of Obstetrics and Gynaecology (Lund) (013018000), Division V (013230900)
id
aebecdd3-9c75-4ca0-a448-afa60e45de0f (old id 397306)
date added to LUP
2016-04-01 11:48:07
date last changed
2020-01-12 08:38:15
@article{aebecdd3-9c75-4ca0-a448-afa60e45de0f,
  abstract     = {Cellular reuptake of monoamines, which is mediated by cell membrane transporters, is followed by accumulation in vesicles by vesicular monoamine transporters (VMAT). The aim of this study was to demonstrate the presence of functional monoamine transporters with high affinity for histamine in human endometrial tissue, since histamine has been implicated as a paracrine signal during endometrial decidualization and embryo implantation. In situ hybridization with S-35-labelled cRNA probes was used for detection of the organic cationic transporter-2 (OCT-2), the extraneuronal monoamine transporter (EMT), and VMAT-2 in cryosections of normal human endometrial tissue. To identify functional transporters for histamine in endometrial cells, we incubated primary cultures of stromal cells and cultures of attached glands with H-3-labelled histamine. Cultures were pretreated with either corticosterone, a specific inhibitor of EMT, or reserpine, a specific inhibitor of VMAT-2. EMT mRNA was localized in the stroma with peak expression in the secretory phase, whereas OCT-2 mRNA was expressed by few cells in the stroma throughout the cycle. VMAT-2 mRNA was localized in the stroma during the proliferative phase and in the epithelium during the secretory phase. Thus, EMT and VMAT-2, which both have high affinity for histamine, are strongly expressed in endometrial cells. Both corticosterone and reserpine significantly reduced the uptake of H-3-histamine in stromal cells during the proliferative as well as the secretory phase. This indicates the presence of functional EMT and VMAT-2 transporter proteins throughout the cycle, even though their periods of maximal mRNA expression were limited. The results of uptake experiments with glandular epithelial cells confirmed not only the presence of functional VMAT-2 transporter protein in the secretory phase but also the absence of a histamine-specific plasma membrane transporter throughout the cycle. Thus, endometrial tissue contains both plasma membrane and vesicular membrane monoamine transporters with high affinity for histamine. They can potentially influence the reproductive process by the uptake of extracellular histamine and subsequent release on demand.},
  author       = {Casslén, Vera and Bottalico, Barbara and Olsson, Hanna and Ehinger, Anna and Pilka, Radovan and Casslén, Bertil and Hansson, Stefan},
  issn         = {1460-2407},
  language     = {eng},
  number       = {8},
  pages        = {483--489},
  publisher    = {Oxford University Press},
  series       = {Molecular Human Reproduction},
  title        = {Histamine uptake by human endometrial cells expressing the organic cation transporter EMT and the vesicular monoamine transporter-2},
  url          = {http://dx.doi.org/10.1093/molehr/gah259},
  doi          = {10.1093/molehr/gah259},
  volume       = {12},
  year         = {2006},
}