Small-angle X-ray and neutron scattering applied to lipid-based nanoparticles : Recent advancements across different length scales
(2024) In Advances in Colloid and Interface Science 327.- Abstract
Lipid-based nanoparticles (LNPs), ranging from nanovesicles to non-lamellar assemblies, have gained significant attention in recent years, as versatile carriers for delivering drugs, vaccines, and nutrients. Small-angle scattering methods, employing X-rays (SAXS) or neutrons (SANS), represent unique tools to unveil structure, dynamics, and interactions of such particles on different length scales, spanning from the nano to the molecular scale. This review explores the state-of-the-art on scattering methods applied to unveil the structure of lipid-based nanoparticles and their interactions with drugs and bioactive molecules, to inform their rational design and formulation for medical applications. We will focus on complementary... (More)
Lipid-based nanoparticles (LNPs), ranging from nanovesicles to non-lamellar assemblies, have gained significant attention in recent years, as versatile carriers for delivering drugs, vaccines, and nutrients. Small-angle scattering methods, employing X-rays (SAXS) or neutrons (SANS), represent unique tools to unveil structure, dynamics, and interactions of such particles on different length scales, spanning from the nano to the molecular scale. This review explores the state-of-the-art on scattering methods applied to unveil the structure of lipid-based nanoparticles and their interactions with drugs and bioactive molecules, to inform their rational design and formulation for medical applications. We will focus on complementary information accessible with X-rays or neutrons, ranging from insights on the structure and colloidal processes at a nanoscale level (SAXS) to details on the lipid organization and molecular interactions of LNPs (SANS). In addition, we will review new opportunities offered by Time-resolved (TR)-SAXS and -SANS for the investigation of dynamic processes involving LNPs. These span from real-time monitoring of LNPs structural evolution in response to endogenous or external stimuli (TR-SANS), to the investigation of the kinetics of lipid diffusion and exchange upon interaction with biomolecules (TR-SANS). Finally, we will spotlight novel combinations of SAXS and SANS with complementary on-line techniques, recently enabled at Large Scale Facilities for X-rays and neutrons. This emerging technology enables synchronized multi-method investigation, offering exciting opportunities for the simultaneous characterization of the structure and chemical or mechanical properties of LNPs.
(Less)
- author
- Caselli, Lucrezia LU ; Conti, Laura ; De Santis, Ilaria and Berti, Debora
- organization
- publishing date
- 2024-05
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Cubosomes, Lipid nanoparticles, Lipid vesicles, Small-angle neutron scattering, Small-angle X-ray scattering
- in
- Advances in Colloid and Interface Science
- volume
- 327
- article number
- 103156
- publisher
- Elsevier
- external identifiers
-
- pmid:38643519
- scopus:85190737828
- ISSN
- 0001-8686
- DOI
- 10.1016/j.cis.2024.103156
- language
- English
- LU publication?
- yes
- id
- 399b57fd-2d9e-49c0-8176-fe6f7a14116b
- date added to LUP
- 2024-04-29 12:27:35
- date last changed
- 2024-05-13 13:45:51
@article{399b57fd-2d9e-49c0-8176-fe6f7a14116b,
abstract = {{<p>Lipid-based nanoparticles (LNPs), ranging from nanovesicles to non-lamellar assemblies, have gained significant attention in recent years, as versatile carriers for delivering drugs, vaccines, and nutrients. Small-angle scattering methods, employing X-rays (SAXS) or neutrons (SANS), represent unique tools to unveil structure, dynamics, and interactions of such particles on different length scales, spanning from the nano to the molecular scale. This review explores the state-of-the-art on scattering methods applied to unveil the structure of lipid-based nanoparticles and their interactions with drugs and bioactive molecules, to inform their rational design and formulation for medical applications. We will focus on complementary information accessible with X-rays or neutrons, ranging from insights on the structure and colloidal processes at a nanoscale level (SAXS) to details on the lipid organization and molecular interactions of LNPs (SANS). In addition, we will review new opportunities offered by Time-resolved (TR)-SAXS and -SANS for the investigation of dynamic processes involving LNPs. These span from real-time monitoring of LNPs structural evolution in response to endogenous or external stimuli (TR-SANS), to the investigation of the kinetics of lipid diffusion and exchange upon interaction with biomolecules (TR-SANS). Finally, we will spotlight novel combinations of SAXS and SANS with complementary on-line techniques, recently enabled at Large Scale Facilities for X-rays and neutrons. This emerging technology enables synchronized multi-method investigation, offering exciting opportunities for the simultaneous characterization of the structure and chemical or mechanical properties of LNPs.</p>}},
author = {{Caselli, Lucrezia and Conti, Laura and De Santis, Ilaria and Berti, Debora}},
issn = {{0001-8686}},
keywords = {{Cubosomes; Lipid nanoparticles; Lipid vesicles; Small-angle neutron scattering; Small-angle X-ray scattering}},
language = {{eng}},
publisher = {{Elsevier}},
series = {{Advances in Colloid and Interface Science}},
title = {{Small-angle X-ray and neutron scattering applied to lipid-based nanoparticles : Recent advancements across different length scales}},
url = {{http://dx.doi.org/10.1016/j.cis.2024.103156}},
doi = {{10.1016/j.cis.2024.103156}},
volume = {{327}},
year = {{2024}},
}