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Quantitative Proteomic Analysis of Cervical Cancer Tissues Identifies Proteins Associated With Cancer Progression

Ramírez-Torres, Alberto ; Gil, Jeovanis LU ; Contreras, Sandra ; Ramírez, Graciela ; Valencia-González, Heriberto A. ; Salazar-Bustamante, Emmanuel ; Gómezcaudillo, Leopoldo ; García-Carranca, Alejandro and Encarnación-Guevara, Sergio (2022) In Cancer Genomics and Proteomics 19(2). p.241-258
Abstract

Background/Aim: To date, several proteomics studies in cervical cancer (CC) have focused mainly on squamous cervical cancer (SCC). Our study aimed to discover and clarify differences in SCC and CAD that may provide valuable information for the identification of proteins involved in tumor progression, in CC as a whole, or specific for SCC or CAD. Materials and Methods: Total protein extracts from 15 individual samples corresponding to 5 different CC tissue types were compared with a non-cancerous control group using bidimensional liquid chromatography-mass spectrometry (2D LC-MS/MS), isobaric tags for relative and absolute quantitation (ITRAQ), principal component analysis (PCA) and gene set enrichment analysis (GSEA). Results: A total... (More)

Background/Aim: To date, several proteomics studies in cervical cancer (CC) have focused mainly on squamous cervical cancer (SCC). Our study aimed to discover and clarify differences in SCC and CAD that may provide valuable information for the identification of proteins involved in tumor progression, in CC as a whole, or specific for SCC or CAD. Materials and Methods: Total protein extracts from 15 individual samples corresponding to 5 different CC tissue types were compared with a non-cancerous control group using bidimensional liquid chromatography-mass spectrometry (2D LC-MS/MS), isobaric tags for relative and absolute quantitation (ITRAQ), principal component analysis (PCA) and gene set enrichment analysis (GSEA). Results: A total of 622 statistically significant different proteins were detected. Exocytosis-related proteins were the most over-represented, accounting for 25% of the identified and quantified proteins. Based on the experimental results, reticulocalbin 3 (RCN3) and Ras-related protein Rab-14 (RAB14) were chosen for further downstream in vitro and vivo analyses. RCN3 was overexpressed in all CC tissues compared to the control and RAB14 was overexpressed in squamous cervical cancer (SCC) compared to invasive cervical adenocarcinoma (CAD). In the tumor xenograft experiment, RAB14 protein expression was positively correlated with increased tumor size. In addition, RCN3-expressing HeLa cells induced a discrete size increment compared to control, at day 47 after inoculation. Conclusion: RAB14 and RCN3 are suggested as potential biomarkers and therapeutic targets in the treatment of CC.

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author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cervical adenocarcinoma, Exosome, ITRAQ, Proteomics, RAB14, RCN3
in
Cancer Genomics and Proteomics
volume
19
issue
2
pages
18 pages
publisher
International Institute of Anticancer Research
external identifiers
  • scopus:85124933192
  • pmid:35181591
ISSN
1109-6535
DOI
10.21873/cgp.20317
language
English
LU publication?
yes
id
39a0859e-2c00-463e-a78d-4590e051bd74
date added to LUP
2022-04-12 14:36:02
date last changed
2024-06-18 03:11:26
@article{39a0859e-2c00-463e-a78d-4590e051bd74,
  abstract     = {{<p>Background/Aim: To date, several proteomics studies in cervical cancer (CC) have focused mainly on squamous cervical cancer (SCC). Our study aimed to discover and clarify differences in SCC and CAD that may provide valuable information for the identification of proteins involved in tumor progression, in CC as a whole, or specific for SCC or CAD. Materials and Methods: Total protein extracts from 15 individual samples corresponding to 5 different CC tissue types were compared with a non-cancerous control group using bidimensional liquid chromatography-mass spectrometry (2D LC-MS/MS), isobaric tags for relative and absolute quantitation (ITRAQ), principal component analysis (PCA) and gene set enrichment analysis (GSEA). Results: A total of 622 statistically significant different proteins were detected. Exocytosis-related proteins were the most over-represented, accounting for 25% of the identified and quantified proteins. Based on the experimental results, reticulocalbin 3 (RCN3) and Ras-related protein Rab-14 (RAB14) were chosen for further downstream in vitro and vivo analyses. RCN3 was overexpressed in all CC tissues compared to the control and RAB14 was overexpressed in squamous cervical cancer (SCC) compared to invasive cervical adenocarcinoma (CAD). In the tumor xenograft experiment, RAB14 protein expression was positively correlated with increased tumor size. In addition, RCN3-expressing HeLa cells induced a discrete size increment compared to control, at day 47 after inoculation. Conclusion: RAB14 and RCN3 are suggested as potential biomarkers and therapeutic targets in the treatment of CC.</p>}},
  author       = {{Ramírez-Torres, Alberto and Gil, Jeovanis and Contreras, Sandra and Ramírez, Graciela and Valencia-González, Heriberto A. and Salazar-Bustamante, Emmanuel and Gómezcaudillo, Leopoldo and García-Carranca, Alejandro and Encarnación-Guevara, Sergio}},
  issn         = {{1109-6535}},
  keywords     = {{Cervical adenocarcinoma; Exosome; ITRAQ; Proteomics; RAB14; RCN3}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{241--258}},
  publisher    = {{International Institute of Anticancer Research}},
  series       = {{Cancer Genomics and Proteomics}},
  title        = {{Quantitative Proteomic Analysis of Cervical Cancer Tissues Identifies Proteins Associated With Cancer Progression}},
  url          = {{http://dx.doi.org/10.21873/cgp.20317}},
  doi          = {{10.21873/cgp.20317}},
  volume       = {{19}},
  year         = {{2022}},
}