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Antifungal activities of extracts produced by liquid fermentations of Chilean Stereum species against Botrytis cinerea (grey mould agent)

Aqueveque, Pedro; Céspedes, Carlos L.; Alarcón, Julio; Schmeda-Hirschmann, Guillermo; Cañumir, Juan A.; Becerra, José; Silva, Mario; Sterner, Olov LU ; Radrigán, Rudy and Aranda, Mario (2016) In Crop Protection 89. p.95-100
Abstract

Extracts obtained from liquid mycelial fermentations of Chilean species of the Stereum genus showed antifungal activity against Botrytis cinerea. Thirty-six strains were evaluated in vivo and in vitro assays, 25 belonging to Stereum hirsutum (Sh) and 11 to Stereum rameale (Sr). Two types of extracts were obtained: EtOAc-extract (liquid phase) and MeOH-extract (mycelial phase). Plate diffusion assay showed that EtOAc-extracts were more active than MeOH-extracts. S. hirsutum included 4 strains with the highest antifungal activity (Sh134-11, Sh144-11, Sh152-11, Sh155-11) and S. rameale highlighted with two strains (Sr25-11, Sr27-11). Effects on the mycelial growth of B. cinerea showed that EtOAc-extracts produced by S. hirsutum (Sh134-11,... (More)

Extracts obtained from liquid mycelial fermentations of Chilean species of the Stereum genus showed antifungal activity against Botrytis cinerea. Thirty-six strains were evaluated in vivo and in vitro assays, 25 belonging to Stereum hirsutum (Sh) and 11 to Stereum rameale (Sr). Two types of extracts were obtained: EtOAc-extract (liquid phase) and MeOH-extract (mycelial phase). Plate diffusion assay showed that EtOAc-extracts were more active than MeOH-extracts. S. hirsutum included 4 strains with the highest antifungal activity (Sh134-11, Sh144-11, Sh152-11, Sh155-11) and S. rameale highlighted with two strains (Sr25-11, Sr27-11). Effects on the mycelial growth of B. cinerea showed that EtOAc-extracts produced by S. hirsutum (Sh134-11, Sh152-11) inhibited from 1000 μg mL−1, reaching 67 and 49%, respectively. At 2000 μg mL−1, these strains inhibited nearly 80% of mycelial growth. EtOAc-extract of Sh134-11 was more effective to control the sporogenesis, inhibiting in 100% the sporulation at 500 μg mL−1. Assays showed that Sh134-11 and Sh152-11 exhibited a minimal fungicidal concentration (MFC) of 50 and 100 μg mL−1 respectively and minimal inhibitory concentration (MIC) at 20 μg mL−1. EtOAc-extracts of Sr25-11 and Sr27-11 showed MFC value at 100 μg mL−1 and MIC at 20 and 50 μg mL−1, respectively. Strawberries treated with 1000 μg mL−1of Sh134-11 and Sr25-11 reached 82 and 72% of decay inhibition, respectively. Treatments with 2000 μg mL−1 showed a decay inhibition of 90% approximately. In vivo tests are in accordance with the results obtained in vitro assays, confirming the efficacy of Sh134-11 and Sr25-11 to control B. cinerea. Differences in antifungal activities observed in the different strains suggested that the ability to produce bioactive compounds is not homogenously distributed among S. hirsutum and S. rameale. Our study would suggest that submerged fermentations of Chilean S. hirsutum strain Sh134-11 produce extracts, which could be used as possible biofungicides and an alternative to synthetic fungicides.

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Contribution to journal
publication status
published
subject
keywords
Antifungal, Botrytis, Chilean fungi, Stereum, Submerged cultures
in
Crop Protection
volume
89
pages
6 pages
publisher
Elsevier
external identifiers
  • Scopus:84978512541
  • WOS:000383826400014
ISSN
0261-2194
DOI
10.1016/j.cropro.2016.07.014
language
English
LU publication?
yes
id
39aaf580-4ad3-4ab4-b1c8-41c751fcb0ed
date added to LUP
2016-10-14 08:28:10
date last changed
2017-01-01 08:36:44
@article{39aaf580-4ad3-4ab4-b1c8-41c751fcb0ed,
  abstract     = {<p>Extracts obtained from liquid mycelial fermentations of Chilean species of the Stereum genus showed antifungal activity against Botrytis cinerea. Thirty-six strains were evaluated in vivo and in vitro assays, 25 belonging to Stereum hirsutum (Sh) and 11 to Stereum rameale (Sr). Two types of extracts were obtained: EtOAc-extract (liquid phase) and MeOH-extract (mycelial phase). Plate diffusion assay showed that EtOAc-extracts were more active than MeOH-extracts. S. hirsutum included 4 strains with the highest antifungal activity (Sh134-11, Sh144-11, Sh152-11, Sh155-11) and S. rameale highlighted with two strains (Sr25-11, Sr27-11). Effects on the mycelial growth of B. cinerea showed that EtOAc-extracts produced by S. hirsutum (Sh134-11, Sh152-11) inhibited from 1000 μg mL<sup>−1</sup>, reaching 67 and 49%, respectively. At 2000 μg mL<sup>−1</sup>, these strains inhibited nearly 80% of mycelial growth. EtOAc-extract of Sh134-11 was more effective to control the sporogenesis, inhibiting in 100% the sporulation at 500 μg mL<sup>−1</sup>. Assays showed that Sh134-11 and Sh152-11 exhibited a minimal fungicidal concentration (MFC) of 50 and 100 μg mL<sup>−1</sup> respectively and minimal inhibitory concentration (MIC) at 20 μg mL<sup>−1</sup>. EtOAc-extracts of Sr25-11 and Sr27-11 showed MFC value at 100 μg mL<sup>−1</sup> and MIC at 20 and 50 μg mL<sup>−1</sup>, respectively. Strawberries treated with 1000 μg mL<sup>−1</sup>of Sh134-11 and Sr25-11 reached 82 and 72% of decay inhibition, respectively. Treatments with 2000 μg mL<sup>−1</sup> showed a decay inhibition of 90% approximately. In vivo tests are in accordance with the results obtained in vitro assays, confirming the efficacy of Sh134-11 and Sr25-11 to control B. cinerea. Differences in antifungal activities observed in the different strains suggested that the ability to produce bioactive compounds is not homogenously distributed among S. hirsutum and S. rameale. Our study would suggest that submerged fermentations of Chilean S. hirsutum strain Sh134-11 produce extracts, which could be used as possible biofungicides and an alternative to synthetic fungicides.</p>},
  author       = {Aqueveque, Pedro and Céspedes, Carlos L. and Alarcón, Julio and Schmeda-Hirschmann, Guillermo and Cañumir, Juan A. and Becerra, José and Silva, Mario and Sterner, Olov and Radrigán, Rudy and Aranda, Mario},
  issn         = {0261-2194},
  keyword      = {Antifungal,Botrytis,Chilean fungi,Stereum,Submerged cultures},
  language     = {eng},
  month        = {11},
  pages        = {95--100},
  publisher    = {Elsevier},
  series       = {Crop Protection},
  title        = {Antifungal activities of extracts produced by liquid fermentations of Chilean Stereum species against Botrytis cinerea (grey mould agent)},
  url          = {http://dx.doi.org/10.1016/j.cropro.2016.07.014},
  volume       = {89},
  year         = {2016},
}