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Regulation of fungal decomposition at single-cell level

Op De Beeck, Michiel LU ; Troein, Carl LU ; Siregar, Syahril LU ; Gentile, Luigi LU ; Abbondanza, Giuseppe LU ; Peterson, Carsten LU ; Persson, Per LU and Tunlid, Anders LU (2020) In ISME Journal
Abstract

Filamentous fungi play a key role as decomposers in Earth’s nutrient cycles. In soils, substrates are heterogeneously distributed in microenvironments. Hence, individual hyphae of a mycelium may experience very different environmental conditions simultaneously. In the current work, we investigated how fungi cope with local environmental variations at single-cell level. We developed a method based on infrared spectroscopy that allows the direct, in-situ chemical imaging of the decomposition activity of individual hyphal tips. Colonies of the ectomycorrhizal Basidiomycete Paxillus involutus were grown on liquid media, while parts of colonies were allowed to colonize lignin patches. Oxidative decomposition of lignin by individual hyphae... (More)

Filamentous fungi play a key role as decomposers in Earth’s nutrient cycles. In soils, substrates are heterogeneously distributed in microenvironments. Hence, individual hyphae of a mycelium may experience very different environmental conditions simultaneously. In the current work, we investigated how fungi cope with local environmental variations at single-cell level. We developed a method based on infrared spectroscopy that allows the direct, in-situ chemical imaging of the decomposition activity of individual hyphal tips. Colonies of the ectomycorrhizal Basidiomycete Paxillus involutus were grown on liquid media, while parts of colonies were allowed to colonize lignin patches. Oxidative decomposition of lignin by individual hyphae growing under different conditions was followed for a period of seven days. We identified two sub-populations of hyphal tips: one with low decomposition activity and one with much higher activity. Active cells secreted more extracellular polymeric substances and oxidized lignin more strongly. The ratio of active to inactive hyphae strongly depended on the environmental conditions in lignin patches, but was further mediated by the decomposition activity of entire mycelia. Phenotypic heterogeneity occurring between genetically identical hyphal tips may be an important strategy for filamentous fungi to cope with heterogeneous and constantly changing soil environments.

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organization
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Contribution to journal
publication status
epub
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in
ISME Journal
publisher
Nature Publishing Group
external identifiers
  • scopus:85077612432
  • pmid:31896790
ISSN
1751-7362
DOI
10.1038/s41396-019-0583-9
project
MICCS - Molecular Interactions Controlling soil Carbon Sequestration
language
English
LU publication?
yes
id
39f9ee6d-1d52-4d18-8d87-0cc3cbda7ccf
date added to LUP
2020-01-28 11:04:54
date last changed
2020-05-26 05:38:45
@article{39f9ee6d-1d52-4d18-8d87-0cc3cbda7ccf,
  abstract     = {<p>Filamentous fungi play a key role as decomposers in Earth’s nutrient cycles. In soils, substrates are heterogeneously distributed in microenvironments. Hence, individual hyphae of a mycelium may experience very different environmental conditions simultaneously. In the current work, we investigated how fungi cope with local environmental variations at single-cell level. We developed a method based on infrared spectroscopy that allows the direct, in-situ chemical imaging of the decomposition activity of individual hyphal tips. Colonies of the ectomycorrhizal Basidiomycete Paxillus involutus were grown on liquid media, while parts of colonies were allowed to colonize lignin patches. Oxidative decomposition of lignin by individual hyphae growing under different conditions was followed for a period of seven days. We identified two sub-populations of hyphal tips: one with low decomposition activity and one with much higher activity. Active cells secreted more extracellular polymeric substances and oxidized lignin more strongly. The ratio of active to inactive hyphae strongly depended on the environmental conditions in lignin patches, but was further mediated by the decomposition activity of entire mycelia. Phenotypic heterogeneity occurring between genetically identical hyphal tips may be an important strategy for filamentous fungi to cope with heterogeneous and constantly changing soil environments.</p>},
  author       = {Op De Beeck, Michiel and Troein, Carl and Siregar, Syahril and Gentile, Luigi and Abbondanza, Giuseppe and Peterson, Carsten and Persson, Per and Tunlid, Anders},
  issn         = {1751-7362},
  language     = {eng},
  month        = {01},
  publisher    = {Nature Publishing Group},
  series       = {ISME Journal},
  title        = {Regulation of fungal decomposition at single-cell level},
  url          = {http://dx.doi.org/10.1038/s41396-019-0583-9},
  doi          = {10.1038/s41396-019-0583-9},
  year         = {2020},
}