One or multiple samplings for flow cytometric DNA analyses in breast cancer-prognostic implications?
(1992) In Cytometry 13(3). p.241-249- Abstract
- Flow cytometric assessments of DNA ploidy status and the S-phase fraction (SPF) have been shown to yield prognostic information in breast cancer. The aim of the present investigation was to elucidate the reproducibility of results with regard to tumor DNA heterogeneity, and to ascertain whether the prognostic value of DNA measurements might be enhanced by analyzing two pieces of a tumor instead of one. Agreement with regard to ploidy status (diploid versus non-diploid) was obtained in 90% of cases (71/79) when two adjacent sections of the tumor were investigated, and in 77% of cases (10/13) when four biopsies from different quadrants of the tumor specimen were investigated. The corresponding figures for agreement in SPF (divided into three... (More)
- Flow cytometric assessments of DNA ploidy status and the S-phase fraction (SPF) have been shown to yield prognostic information in breast cancer. The aim of the present investigation was to elucidate the reproducibility of results with regard to tumor DNA heterogeneity, and to ascertain whether the prognostic value of DNA measurements might be enhanced by analyzing two pieces of a tumor instead of one. Agreement with regard to ploidy status (diploid versus non-diploid) was obtained in 90% of cases (71/79) when two adjacent sections of the tumor were investigated, and in 77% of cases (10/13) when four biopsies from different quadrants of the tumor specimen were investigated. The corresponding figures for agreement in SPF (divided into three categories, less than 7.0%, 7.0-11.9%, and greater than or equal to 12%) were 75% (59/79; 2-sample series) and 55% (7/13; 4-biopsy series). The main reason for variance in ploidy results was the difficulties in distinguishing near diploid cell populations. Discrepancies in SPF categories could be explained by minor fluctuations in SPF values near the cut-off levels, or by variance in ploidy status, the fraction of non-diploid nuclei, and background noise due to cell debris. There was a stepwise increase in recurrence rate (RR) among patients with increasing SPF category (RR: 20%, 41%, and 53%). Patients whose SPF categories varied, from low or intermediate in one part of the tumor to high in another, seemed to have a poor prognosis (RR = 57%).(ABSTRACT TRUNCATED AT 250 WORDS) (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1106249
- author
- Fernö, Mårten LU ; Baldetorp, Bo LU ; Ewers, Sven-Börje LU ; Idvall, Ingrid ; Olsson, Håkan LU ; Sigurdsson, Helgi and Killander, Dick LU
- organization
- publishing date
- 1992
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- flow cytometry, ploidy, Breast cancer, S-phase, interphase, proliferation, heterogeneity, prognosis
- in
- Cytometry
- volume
- 13
- issue
- 3
- pages
- 241 - 249
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:1576890
- scopus:0026603240
- ISSN
- 0196-4763
- DOI
- 10.1002/cyto.990130305
- language
- English
- LU publication?
- yes
- id
- 3a73b292-ed80-4388-a8c0-65034bc55c4d (old id 1106249)
- date added to LUP
- 2016-04-01 16:53:46
- date last changed
- 2021-01-03 11:12:00
@article{3a73b292-ed80-4388-a8c0-65034bc55c4d, abstract = {{Flow cytometric assessments of DNA ploidy status and the S-phase fraction (SPF) have been shown to yield prognostic information in breast cancer. The aim of the present investigation was to elucidate the reproducibility of results with regard to tumor DNA heterogeneity, and to ascertain whether the prognostic value of DNA measurements might be enhanced by analyzing two pieces of a tumor instead of one. Agreement with regard to ploidy status (diploid versus non-diploid) was obtained in 90% of cases (71/79) when two adjacent sections of the tumor were investigated, and in 77% of cases (10/13) when four biopsies from different quadrants of the tumor specimen were investigated. The corresponding figures for agreement in SPF (divided into three categories, less than 7.0%, 7.0-11.9%, and greater than or equal to 12%) were 75% (59/79; 2-sample series) and 55% (7/13; 4-biopsy series). The main reason for variance in ploidy results was the difficulties in distinguishing near diploid cell populations. Discrepancies in SPF categories could be explained by minor fluctuations in SPF values near the cut-off levels, or by variance in ploidy status, the fraction of non-diploid nuclei, and background noise due to cell debris. There was a stepwise increase in recurrence rate (RR) among patients with increasing SPF category (RR: 20%, 41%, and 53%). Patients whose SPF categories varied, from low or intermediate in one part of the tumor to high in another, seemed to have a poor prognosis (RR = 57%).(ABSTRACT TRUNCATED AT 250 WORDS)}}, author = {{Fernö, Mårten and Baldetorp, Bo and Ewers, Sven-Börje and Idvall, Ingrid and Olsson, Håkan and Sigurdsson, Helgi and Killander, Dick}}, issn = {{0196-4763}}, keywords = {{flow cytometry; ploidy; Breast cancer; S-phase; interphase; proliferation; heterogeneity; prognosis}}, language = {{eng}}, number = {{3}}, pages = {{241--249}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Cytometry}}, title = {{One or multiple samplings for flow cytometric DNA analyses in breast cancer-prognostic implications?}}, url = {{http://dx.doi.org/10.1002/cyto.990130305}}, doi = {{10.1002/cyto.990130305}}, volume = {{13}}, year = {{1992}}, }