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Calmodulin‐Binding Proteins Within the Slow Phase of Axonal Transport in the Rabbit Vagus Nerve Per Ekstrom and Martin Kanje

Ekström, Per LU and Kanje, Martin LU (1987) In Journal of Neurochemistry 49(1). p.146-151
Abstract

Abstract: : Calmodulin‐binding proteins (CBPs) in the rabbit vagus nerve were studied by means of calmodulin‐Sepha‐rose affinity chromatography and polyacrylamide gel electrophoresis. The soluble fraction (105g supernatant) of a nerve homogenate contained four CBPs with molecular weights of 44, 55, 91, and 93 kD, respectively. Slowly transported proteins were recovered in the vagus 3 days after injection of [35S]methionine into the nodose ganglion. Four labelled CBPs with molecular weights of 44, 55, 69, and 83 kD, respectively were found. The nodose ganglion con tained two labelled CBPs, 44 and 55 kD. The 55‐kD CBP was identified as tubulin after immunoblotting. In separate experiments it was also shown that... (More)

Abstract: : Calmodulin‐binding proteins (CBPs) in the rabbit vagus nerve were studied by means of calmodulin‐Sepha‐rose affinity chromatography and polyacrylamide gel electrophoresis. The soluble fraction (105g supernatant) of a nerve homogenate contained four CBPs with molecular weights of 44, 55, 91, and 93 kD, respectively. Slowly transported proteins were recovered in the vagus 3 days after injection of [35S]methionine into the nodose ganglion. Four labelled CBPs with molecular weights of 44, 55, 69, and 83 kD, respectively were found. The nodose ganglion con tained two labelled CBPs, 44 and 55 kD. The 55‐kD CBP was identified as tubulin after immunoblotting. In separate experiments it was also shown that bovine brain tubulin bound to the calmodulin column. Copyright © 1987, Wiley Blackwell. All rights reserved

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Calmodulin, Calmodulin‐binding proteins, Rabbit vagus nerve, Slow axonal transport, Tubulin
in
Journal of Neurochemistry
volume
49
issue
1
pages
6 pages
publisher
Wiley-Blackwell
external identifiers
  • scopus:0023262112
ISSN
0022-3042
DOI
10.1111/j.1471-4159.1987.tb03406.x
language
English
LU publication?
yes
id
3b5d8be1-afc3-46a5-8328-41499f813d13
date added to LUP
2016-12-07 14:28:40
date last changed
2017-01-25 16:27:49
@article{3b5d8be1-afc3-46a5-8328-41499f813d13,
  abstract     = {<p>Abstract:  : Calmodulin‐binding proteins (CBPs) in the rabbit vagus nerve were studied by means of calmodulin‐Sepha‐rose affinity chromatography and polyacrylamide gel electrophoresis. The soluble fraction (10<sup>5</sup>g supernatant) of a nerve homogenate contained four CBPs with molecular weights of 44, 55, 91, and 93 kD, respectively. Slowly transported proteins were recovered in the vagus 3 days after injection of [<sup>35</sup>S]methionine into the nodose ganglion. Four labelled CBPs with molecular weights of 44, 55, 69, and 83 kD, respectively were found. The nodose ganglion con tained two labelled CBPs, 44 and 55 kD. The 55‐kD CBP was identified as tubulin after immunoblotting. In separate experiments it was also shown that bovine brain tubulin bound to the calmodulin column. Copyright © 1987, Wiley Blackwell. All rights reserved</p>},
  author       = {Ekström, Per and Kanje, Martin},
  issn         = {0022-3042},
  keyword      = {Calmodulin,Calmodulin‐binding proteins,Rabbit vagus nerve,Slow axonal transport,Tubulin},
  language     = {eng},
  number       = {1},
  pages        = {146--151},
  publisher    = {Wiley-Blackwell},
  series       = {Journal of Neurochemistry},
  title        = {Calmodulin‐Binding Proteins Within the Slow Phase of Axonal Transport in the Rabbit Vagus Nerve Per Ekstrom and Martin Kanje},
  url          = {http://dx.doi.org/10.1111/j.1471-4159.1987.tb03406.x},
  volume       = {49},
  year         = {1987},
}