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Time-resolved small-angle neutron scattering as a probe for the dynamics of lipid exchange between human lipoproteins and naturally derived membranes

Maric, Selma LU ; Lind, Tania Kjellerup LU ; Raida, Manfred Roman ; Bengtsson, Eva LU orcid ; Fredrikson, Gunilla Nordin LU ; Rogers, Sarah ; Moulin, Martine ; Haertlein, Michael ; Forsyth, V. Trevor and Wenk, Markus R. , et al. (2019) In Scientific Reports 9(1).
Abstract

Atherosclerosis is the main killer in the western world. Today’s clinical markers include the total level of cholesterol and high-/low-density lipoproteins, which often fails to accurately predict the disease. The relationship between the lipid exchange capacity and lipoprotein structure should explain the extent by which they release or accept lipid cargo and should relate to the risk for developing atherosclerosis. Here, small-angle neutron scattering and tailored deuteration have been used to follow the molecular lipid exchange between human lipoprotein particles and cellular membrane mimics made of natural, “neutron invisible” phosphatidylcholines. We show that lipid exchange occurs via two different processes that include lipid... (More)

Atherosclerosis is the main killer in the western world. Today’s clinical markers include the total level of cholesterol and high-/low-density lipoproteins, which often fails to accurately predict the disease. The relationship between the lipid exchange capacity and lipoprotein structure should explain the extent by which they release or accept lipid cargo and should relate to the risk for developing atherosclerosis. Here, small-angle neutron scattering and tailored deuteration have been used to follow the molecular lipid exchange between human lipoprotein particles and cellular membrane mimics made of natural, “neutron invisible” phosphatidylcholines. We show that lipid exchange occurs via two different processes that include lipid transfer via collision and upon direct particle tethering to the membrane, and that high-density lipoprotein excels at exchanging the human-like unsaturated phosphatidylcholine. By mapping the specific lipid content and level of glycation/oxidation, the mode of action of specific lipoproteins can now be deciphered. This information can prove important for the development of improved diagnostic tools and in the treatment of atherosclerosis.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Scientific Reports
volume
9
issue
1
article number
7591
publisher
Nature Publishing Group
external identifiers
  • scopus:85066021599
  • pmid:31110185
ISSN
2045-2322
DOI
10.1038/s41598-019-43713-6
language
English
LU publication?
yes
id
3b6c0382-4eb0-4a5c-968f-e99ce7c67e49
date added to LUP
2019-06-11 08:21:15
date last changed
2025-01-09 13:11:37
@article{3b6c0382-4eb0-4a5c-968f-e99ce7c67e49,
  abstract     = {{<p>Atherosclerosis is the main killer in the western world. Today’s clinical markers include the total level of cholesterol and high-/low-density lipoproteins, which often fails to accurately predict the disease. The relationship between the lipid exchange capacity and lipoprotein structure should explain the extent by which they release or accept lipid cargo and should relate to the risk for developing atherosclerosis. Here, small-angle neutron scattering and tailored deuteration have been used to follow the molecular lipid exchange between human lipoprotein particles and cellular membrane mimics made of natural, “neutron invisible” phosphatidylcholines. We show that lipid exchange occurs via two different processes that include lipid transfer via collision and upon direct particle tethering to the membrane, and that high-density lipoprotein excels at exchanging the human-like unsaturated phosphatidylcholine. By mapping the specific lipid content and level of glycation/oxidation, the mode of action of specific lipoproteins can now be deciphered. This information can prove important for the development of improved diagnostic tools and in the treatment of atherosclerosis.</p>}},
  author       = {{Maric, Selma and Lind, Tania Kjellerup and Raida, Manfred Roman and Bengtsson, Eva and Fredrikson, Gunilla Nordin and Rogers, Sarah and Moulin, Martine and Haertlein, Michael and Forsyth, V. Trevor and Wenk, Markus R. and Pomorski, Thomas Günther and Arnebrant, Thomas and Lund, Reidar and Cárdenas, Marité}},
  issn         = {{2045-2322}},
  language     = {{eng}},
  month        = {{05}},
  number       = {{1}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Scientific Reports}},
  title        = {{Time-resolved small-angle neutron scattering as a probe for the dynamics of lipid exchange between human lipoproteins and naturally derived membranes}},
  url          = {{http://dx.doi.org/10.1038/s41598-019-43713-6}},
  doi          = {{10.1038/s41598-019-43713-6}},
  volume       = {{9}},
  year         = {{2019}},
}