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Acoustic separation of bacteria from blood cells at high cell concentrations enabled by acoustic impedance matched buffers

Ohlsson, Pelle LU ; Petersson, Klara LU and Laurell, Thomas LU (2014) 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 p.388-390
Abstract

We have developed a method to separate bacteria from red and white blood cells (henceforth collectively called blood cells) using acoustophoresis, for subsequent PCR detection. To maximize throughput, we have now investigated the effect of increased cell concentration on the separation. Increasing the blood concentration drastically decreases bacteria recovery, but we show that this can be remedied by optimizing the acoustic impedance of the center buffer. This way we can recover 89% of the bacteria while removing more than 99.8% of the blood cells from 1 ml whole blood within 25 min, paving the way for new sepsis diagnostics methods.

Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Acoustophoresis, Bacteria, Blood, Sepsis
host publication
18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
pages
3 pages
publisher
Chemical and Biological Microsystems Society
conference name
18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
conference location
San Antonio, United States
conference dates
2014-10-26 - 2014-10-30
external identifiers
  • scopus:84941626775
ISBN
9780979806476
language
English
LU publication?
yes
id
3bba6caf-2420-4fa4-9b00-7911d4ce5366
date added to LUP
2017-12-06 14:26:46
date last changed
2019-01-06 13:35:52
@inproceedings{3bba6caf-2420-4fa4-9b00-7911d4ce5366,
  abstract     = {<p>We have developed a method to separate bacteria from red and white blood cells (henceforth collectively called blood cells) using acoustophoresis, for subsequent PCR detection. To maximize throughput, we have now investigated the effect of increased cell concentration on the separation. Increasing the blood concentration drastically decreases bacteria recovery, but we show that this can be remedied by optimizing the acoustic impedance of the center buffer. This way we can recover 89% of the bacteria while removing more than 99.8% of the blood cells from 1 ml whole blood within 25 min, paving the way for new sepsis diagnostics methods.</p>},
  author       = {Ohlsson, Pelle and Petersson, Klara and Laurell, Thomas},
  isbn         = {9780979806476},
  keyword      = {Acoustophoresis,Bacteria,Blood,Sepsis},
  language     = {eng},
  location     = {San Antonio, United States},
  pages        = {388--390},
  publisher    = {Chemical and Biological Microsystems Society},
  title        = {Acoustic separation of bacteria from blood cells at high cell concentrations enabled by acoustic impedance matched buffers},
  year         = {2014},
}