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Perspectives of targeted mass spectrometry for protein biomarker verification

Hüttenhain, Ruth; Malmström, Johan LU ; Picotti, Paola and Aebersold, Ruedi (2009) In Current Opinion in Chemical Biology 13(5-6). p.25-518
Abstract

The identification of specific biomarkers will improve the early diagnosis of disease, facilitate the development of targeted therapies, and provide an accurate method to monitor treatment response. A major challenge in the process of verifying biomarker candidates in blood plasma is the complexity and high dynamic range of proteins. This article reviews the current, targeted proteomic strategies that are capable of quantifying biomarker candidates at concentration ranges where biomarkers are expected in plasma (i.e. at the ng/ml level). In addition, a workflow is presented that allows the fast and definitive generation of targeted mass spectrometry-based assays for most biomarker candidate proteins. These assays are stored in publicly... (More)

The identification of specific biomarkers will improve the early diagnosis of disease, facilitate the development of targeted therapies, and provide an accurate method to monitor treatment response. A major challenge in the process of verifying biomarker candidates in blood plasma is the complexity and high dynamic range of proteins. This article reviews the current, targeted proteomic strategies that are capable of quantifying biomarker candidates at concentration ranges where biomarkers are expected in plasma (i.e. at the ng/ml level). In addition, a workflow is presented that allows the fast and definitive generation of targeted mass spectrometry-based assays for most biomarker candidate proteins. These assays are stored in publicly accessible databases and have the potential to greatly impact the throughput of biomarker verification studies.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Animals, Biomarkers, Blood Proteins, Databases, Protein, Humans, Mass Spectrometry, Proteins, Proteomics, Reproducibility of Results, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Review
in
Current Opinion in Chemical Biology
volume
13
issue
5-6
pages
8 pages
publisher
Elsevier
external identifiers
  • scopus:70549113220
ISSN
1879-0402
DOI
10.1016/j.cbpa.2009.09.014
language
English
LU publication?
no
id
3c02c8b5-613e-4407-a9d7-c17b49d9528a
date added to LUP
2017-09-04 17:20:33
date last changed
2017-10-22 05:35:30
@article{3c02c8b5-613e-4407-a9d7-c17b49d9528a,
  abstract     = {<p>The identification of specific biomarkers will improve the early diagnosis of disease, facilitate the development of targeted therapies, and provide an accurate method to monitor treatment response. A major challenge in the process of verifying biomarker candidates in blood plasma is the complexity and high dynamic range of proteins. This article reviews the current, targeted proteomic strategies that are capable of quantifying biomarker candidates at concentration ranges where biomarkers are expected in plasma (i.e. at the ng/ml level). In addition, a workflow is presented that allows the fast and definitive generation of targeted mass spectrometry-based assays for most biomarker candidate proteins. These assays are stored in publicly accessible databases and have the potential to greatly impact the throughput of biomarker verification studies.</p>},
  author       = {Hüttenhain, Ruth and Malmström, Johan and Picotti, Paola and Aebersold, Ruedi},
  issn         = {1879-0402},
  keyword      = {Animals,Biomarkers,Blood Proteins,Databases, Protein,Humans,Mass Spectrometry,Proteins,Proteomics,Reproducibility of Results,Journal Article,Research Support, N.I.H., Extramural,Research Support, Non-U.S. Gov't,Review},
  language     = {eng},
  number       = {5-6},
  pages        = {25--518},
  publisher    = {Elsevier},
  series       = {Current Opinion in Chemical Biology},
  title        = {Perspectives of targeted mass spectrometry for protein biomarker verification},
  url          = {http://dx.doi.org/10.1016/j.cbpa.2009.09.014},
  volume       = {13},
  year         = {2009},
}