Perspectives of targeted mass spectrometry for protein biomarker verification
Hüttenhain, Ruth ; Malmström, Johan LU
; Picotti, Paola
and Aebersold, Ruedi
(2009)
In Current Opinion in Chemical Biology
13(5-6).
p.25-518
- Abstract
The identification of specific biomarkers will improve the early diagnosis of disease, facilitate the development of targeted therapies, and provide an accurate method to monitor treatment response. A major challenge in the process of verifying biomarker candidates in blood plasma is the complexity and high dynamic range of proteins. This article reviews the current, targeted proteomic strategies that are capable of quantifying biomarker candidates at concentration ranges where biomarkers are expected in plasma (i.e. at the ng/ml level). In addition, a workflow is presented that allows the fast and definitive generation of targeted mass spectrometry-based assays for most biomarker candidate proteins. These assays are stored in publicly... (More)
The identification of specific biomarkers will improve the early diagnosis of disease, facilitate the development of targeted therapies, and provide an accurate method to monitor treatment response. A major challenge in the process of verifying biomarker candidates in blood plasma is the complexity and high dynamic range of proteins. This article reviews the current, targeted proteomic strategies that are capable of quantifying biomarker candidates at concentration ranges where biomarkers are expected in plasma (i.e. at the ng/ml level). In addition, a workflow is presented that allows the fast and definitive generation of targeted mass spectrometry-based assays for most biomarker candidate proteins. These assays are stored in publicly accessible databases and have the potential to greatly impact the throughput of biomarker verification studies.
(Less)
- author
- Hüttenhain, Ruth
; Malmström, Johan
LU
; Picotti, Paola
and Aebersold, Ruedi
- publishing date
- 2009-12
- type
- Contribution to journal
- publication status
- published
- keywords
- Animals, Biomarkers, Blood Proteins, Databases, Protein, Humans, Mass Spectrometry, Proteins, Proteomics, Reproducibility of Results, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Review
- in
- Current Opinion in Chemical Biology
- volume
- 13
- issue
- 5-6
- pages
- 8 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:70549113220
- pmid:19818677
- ISSN
- 1879-0402
- DOI
- 10.1016/j.cbpa.2009.09.014
- language
- English
- LU publication?
- no
- id
- 3c02c8b5-613e-4407-a9d7-c17b49d9528a
- date added to LUP
- 2017-09-04 17:20:33
- date last changed
- 2024-03-31 16:04:36
@article{3c02c8b5-613e-4407-a9d7-c17b49d9528a,
abstract = {{<p>The identification of specific biomarkers will improve the early diagnosis of disease, facilitate the development of targeted therapies, and provide an accurate method to monitor treatment response. A major challenge in the process of verifying biomarker candidates in blood plasma is the complexity and high dynamic range of proteins. This article reviews the current, targeted proteomic strategies that are capable of quantifying biomarker candidates at concentration ranges where biomarkers are expected in plasma (i.e. at the ng/ml level). In addition, a workflow is presented that allows the fast and definitive generation of targeted mass spectrometry-based assays for most biomarker candidate proteins. These assays are stored in publicly accessible databases and have the potential to greatly impact the throughput of biomarker verification studies.</p>}},
author = {{Hüttenhain, Ruth and Malmström, Johan and Picotti, Paola and Aebersold, Ruedi}},
issn = {{1879-0402}},
keywords = {{Animals; Biomarkers; Blood Proteins; Databases, Protein; Humans; Mass Spectrometry; Proteins; Proteomics; Reproducibility of Results; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Review}},
language = {{eng}},
number = {{5-6}},
pages = {{25--518}},
publisher = {{Elsevier}},
series = {{Current Opinion in Chemical Biology}},
title = {{Perspectives of targeted mass spectrometry for protein biomarker verification}},
url = {{http://dx.doi.org/10.1016/j.cbpa.2009.09.014}},
doi = {{10.1016/j.cbpa.2009.09.014}},
volume = {{13}},
year = {{2009}},
}