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Upregulation of cytosolic phospholipase A2 correlates with apoptosis in mouse superior cervical and dorsal root ganglia neurons

Hornfelt, M. LU ; Edström, A. LU and Ekström, Per LU (1999) In Neuroscience Letters 265(2). p.87-90
Abstract

The involvement of cytosolic phospholipase A2 (cPLA2) in apoptosis of adult mouse superior cervical and dorsal root ganglia neurons has been investigated by the use of immunohistochemistry for cPLA2 and DNA nick-end labeling for apoptotic cells, respectively, cPLA2 immunoreactivity was strongly upregulated in neurons of both preparations during in vitro culturing. By double labeling it was unequivocally demonstrated that cPLA2 was present and upregulated only in neurons undergoing apoptosis. A similar picture emerged when cPLA2 immunoreactivity was compared with staining with Fluoro-Jade, a novel fluorochrome marker for neuronal degeneration. The preferential presence of... (More)

The involvement of cytosolic phospholipase A2 (cPLA2) in apoptosis of adult mouse superior cervical and dorsal root ganglia neurons has been investigated by the use of immunohistochemistry for cPLA2 and DNA nick-end labeling for apoptotic cells, respectively, cPLA2 immunoreactivity was strongly upregulated in neurons of both preparations during in vitro culturing. By double labeling it was unequivocally demonstrated that cPLA2 was present and upregulated only in neurons undergoing apoptosis. A similar picture emerged when cPLA2 immunoreactivity was compared with staining with Fluoro-Jade, a novel fluorochrome marker for neuronal degeneration. The preferential presence of cPLA2 in apoptotic and degenerating cells suggests that the enzyme is important for some mechanism involved in or intimately coupled to neuronal cell death.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Adult, Apoptosis, Cell death, cPLA, Dorsal root ganglia, Mouse, Superior cervical ganglia
in
Neuroscience Letters
volume
265
issue
2
pages
4 pages
publisher
Elsevier
external identifiers
  • Scopus:0033574671
ISSN
0304-3940
DOI
10.1016/S0304-3940(99)00046-4
language
English
LU publication?
yes
id
3c1d0d1b-0530-4d68-8c34-76c362110061
date added to LUP
2016-12-07 14:53:31
date last changed
2017-01-20 16:30:53
@article{3c1d0d1b-0530-4d68-8c34-76c362110061,
  abstract     = {<p>The involvement of cytosolic phospholipase A<sub>2</sub> (cPLA<sub>2</sub>) in apoptosis of adult mouse superior cervical and dorsal root ganglia neurons has been investigated by the use of immunohistochemistry for cPLA<sub>2</sub> and DNA nick-end labeling for apoptotic cells, respectively, cPLA<sub>2</sub> immunoreactivity was strongly upregulated in neurons of both preparations during in vitro culturing. By double labeling it was unequivocally demonstrated that cPLA<sub>2</sub> was present and upregulated only in neurons undergoing apoptosis. A similar picture emerged when cPLA<sub>2</sub> immunoreactivity was compared with staining with Fluoro-Jade, a novel fluorochrome marker for neuronal degeneration. The preferential presence of cPLA<sub>2</sub> in apoptotic and degenerating cells suggests that the enzyme is important for some mechanism involved in or intimately coupled to neuronal cell death.</p>},
  author       = {Hornfelt, M. and Edström, A. and Ekström, Per},
  issn         = {0304-3940},
  keyword      = {Adult,Apoptosis,Cell death,cPLA,Dorsal root ganglia,Mouse,Superior cervical ganglia},
  language     = {eng},
  month        = {04},
  number       = {2},
  pages        = {87--90},
  publisher    = {Elsevier},
  series       = {Neuroscience Letters},
  title        = {Upregulation of cytosolic phospholipase A2 correlates with apoptosis in mouse superior cervical and dorsal root ganglia neurons},
  url          = {http://dx.doi.org/10.1016/S0304-3940(99)00046-4},
  volume       = {265},
  year         = {1999},
}