Upregulation of cytosolic phospholipase A2 correlates with apoptosis in mouse superior cervical and dorsal root ganglia neurons
(1999) In Neuroscience Letters 265(2). p.87-90- Abstract
The involvement of cytosolic phospholipase A2 (cPLA2) in apoptosis of adult mouse superior cervical and dorsal root ganglia neurons has been investigated by the use of immunohistochemistry for cPLA2 and DNA nick-end labeling for apoptotic cells, respectively, cPLA2 immunoreactivity was strongly upregulated in neurons of both preparations during in vitro culturing. By double labeling it was unequivocally demonstrated that cPLA2 was present and upregulated only in neurons undergoing apoptosis. A similar picture emerged when cPLA2 immunoreactivity was compared with staining with Fluoro-Jade, a novel fluorochrome marker for neuronal degeneration. The preferential presence of... (More)
The involvement of cytosolic phospholipase A2 (cPLA2) in apoptosis of adult mouse superior cervical and dorsal root ganglia neurons has been investigated by the use of immunohistochemistry for cPLA2 and DNA nick-end labeling for apoptotic cells, respectively, cPLA2 immunoreactivity was strongly upregulated in neurons of both preparations during in vitro culturing. By double labeling it was unequivocally demonstrated that cPLA2 was present and upregulated only in neurons undergoing apoptosis. A similar picture emerged when cPLA2 immunoreactivity was compared with staining with Fluoro-Jade, a novel fluorochrome marker for neuronal degeneration. The preferential presence of cPLA2 in apoptotic and degenerating cells suggests that the enzyme is important for some mechanism involved in or intimately coupled to neuronal cell death.
(Less)
- author
- Hornfelt, M. LU ; Edström, A. LU and Ekström, Per LU
- organization
- publishing date
- 1999-04-16
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Adult, Apoptosis, Cell death, cPLA, Dorsal root ganglia, Mouse, Superior cervical ganglia
- in
- Neuroscience Letters
- volume
- 265
- issue
- 2
- pages
- 4 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:0033574671
- pmid:10327175
- ISSN
- 0304-3940
- DOI
- 10.1016/S0304-3940(99)00046-4
- language
- English
- LU publication?
- yes
- id
- 3c1d0d1b-0530-4d68-8c34-76c362110061
- date added to LUP
- 2016-12-07 14:53:31
- date last changed
- 2025-01-12 17:09:48
@article{3c1d0d1b-0530-4d68-8c34-76c362110061,
abstract = {{<p>The involvement of cytosolic phospholipase A<sub>2</sub> (cPLA<sub>2</sub>) in apoptosis of adult mouse superior cervical and dorsal root ganglia neurons has been investigated by the use of immunohistochemistry for cPLA<sub>2</sub> and DNA nick-end labeling for apoptotic cells, respectively, cPLA<sub>2</sub> immunoreactivity was strongly upregulated in neurons of both preparations during in vitro culturing. By double labeling it was unequivocally demonstrated that cPLA<sub>2</sub> was present and upregulated only in neurons undergoing apoptosis. A similar picture emerged when cPLA<sub>2</sub> immunoreactivity was compared with staining with Fluoro-Jade, a novel fluorochrome marker for neuronal degeneration. The preferential presence of cPLA<sub>2</sub> in apoptotic and degenerating cells suggests that the enzyme is important for some mechanism involved in or intimately coupled to neuronal cell death.</p>}},
author = {{Hornfelt, M. and Edström, A. and Ekström, Per}},
issn = {{0304-3940}},
keywords = {{Adult; Apoptosis; Cell death; cPLA; Dorsal root ganglia; Mouse; Superior cervical ganglia}},
language = {{eng}},
month = {{04}},
number = {{2}},
pages = {{87--90}},
publisher = {{Elsevier}},
series = {{Neuroscience Letters}},
title = {{Upregulation of cytosolic phospholipase A2 correlates with apoptosis in mouse superior cervical and dorsal root ganglia neurons}},
url = {{http://dx.doi.org/10.1016/S0304-3940(99)00046-4}},
doi = {{10.1016/S0304-3940(99)00046-4}},
volume = {{265}},
year = {{1999}},
}