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AFM study of lipid monolayers : III. Phase behavior of ceramides, cholesterol and fatty acids

Sparr, E. LU ; Eriksson, L. LU ; Bouwstra, J. A. and Ekelund, K. LU (2001) In Langmuir 17(1). p.164-172
Abstract

The outer part of the skin, stratum corneum, is essential to the skin's barrier function. Monolayer and bulk phase behavior of stratum corneum model lipids have thus been studied. Domain formation in Langmuir-Blodgett monolayers of synthetic ceramides (C16CerIII and C24CerIII), cholesterol, and free fatty acids (lignoceric acid, C24:0, and palmitic acid, C16:0) were investigated by atomic force microscopy. Binary, ternary, and more complex lipid mixtures were examined. It was shown that small amounts of ceramide are miscible in a cholesterol-rich phase with the miscibility dependent upon the ceramide chain length. Two phases are formed at low and intermediate cholesterol concentrations. In the ceramide-cholesterol... (More)

The outer part of the skin, stratum corneum, is essential to the skin's barrier function. Monolayer and bulk phase behavior of stratum corneum model lipids have thus been studied. Domain formation in Langmuir-Blodgett monolayers of synthetic ceramides (C16CerIII and C24CerIII), cholesterol, and free fatty acids (lignoceric acid, C24:0, and palmitic acid, C16:0) were investigated by atomic force microscopy. Binary, ternary, and more complex lipid mixtures were examined. It was shown that small amounts of ceramide are miscible in a cholesterol-rich phase with the miscibility dependent upon the ceramide chain length. Two phases are formed at low and intermediate cholesterol concentrations. In the ceramide-cholesterol monolayers, very small rectangular-shaped ceramide domains thought to be two-dimensional single ceramide crystals are formed. Small domains were also found in more complex mixtures where the fatty acid is miscible in the ceramide phase, although these domains were not as regular in shape. Binary ceramide-cholesterol as well as ternary ceramide-cholesterol-lignoceric acid bulk mixtures were also studied by X-ray diffraction. The bulk lipid miscibility is consistent with the monolayer results.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Langmuir
volume
17
issue
1
pages
9 pages
publisher
The American Chemical Society (ACS)
external identifiers
  • scopus:0035124536
ISSN
0743-7463
DOI
10.1021/la000271n
language
English
LU publication?
yes
id
3c67aa6a-2cd3-4bc2-ad0a-6ff594bdb43b
date added to LUP
2023-12-07 14:57:06
date last changed
2023-12-08 14:54:47
@article{3c67aa6a-2cd3-4bc2-ad0a-6ff594bdb43b,
  abstract     = {{<p>The outer part of the skin, <i>stratum corneum</i>, is essential to the skin's barrier function. Monolayer and bulk phase behavior of <i>stratum corneum</i> model lipids have thus been studied. Domain formation in Langmuir-Blodgett monolayers of synthetic ceramides (C16CerIII and C24CerIII), cholesterol, and free fatty acids (lignoceric acid, C24:0, and palmitic acid, C16:0) were investigated by atomic force microscopy. Binary, ternary, and more complex lipid mixtures were examined. It was shown that small amounts of ceramide are miscible in a cholesterol-rich phase with the miscibility dependent upon the ceramide chain length. Two phases are formed at low and intermediate cholesterol concentrations. In the ceramide-cholesterol monolayers, very small rectangular-shaped ceramide domains thought to be two-dimensional single ceramide crystals are formed. Small domains were also found in more complex mixtures where the fatty acid is miscible in the ceramide phase, although these domains were not as regular in shape. Binary ceramide-cholesterol as well as ternary ceramide-cholesterol-lignoceric acid bulk mixtures were also studied by X-ray diffraction. The bulk lipid miscibility is consistent with the monolayer results.</p>}},
  author       = {{Sparr, E. and Eriksson, L. and Bouwstra, J. A. and Ekelund, K.}},
  issn         = {{0743-7463}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{1}},
  pages        = {{164--172}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{Langmuir}},
  title        = {{AFM study of lipid monolayers : III. Phase behavior of ceramides, cholesterol and fatty acids}},
  url          = {{http://dx.doi.org/10.1021/la000271n}},
  doi          = {{10.1021/la000271n}},
  volume       = {{17}},
  year         = {{2001}},
}