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Enantioselectivity of lipases : Effects of water activity

Wehtje, Ernst LU ; Costes, David LU and Adlercreutz, Patrick LU (1997) In Journal of Molecular Catalysis - B Enzymatic 3(5). p.221-230
Abstract

The enantioselectivity (E) of lipases in esterifications of secondary alcohols with decanoic acid was studied in organic media. The enantioselectivity of 2-octanol differed greatly among the lipases used. Candida antarctica lipase was extremely selective (E= 9 000) while Candida rugosa lipase was much less selective (E= 1.7). Other enzymes (Lipozyme and lipases from Pseudomonas and Rhizopus arrhizus) had intermediate selectivities. In all cases the enantioselectivity for an enzyme was unaffected by changes in water activity. Different methods of determining the enantioselectivity was used: reactions using single enantiomers as well as racemic mixtures. The effect of water activity on enantioselectivity and the enanatioselectivity values... (More)

The enantioselectivity (E) of lipases in esterifications of secondary alcohols with decanoic acid was studied in organic media. The enantioselectivity of 2-octanol differed greatly among the lipases used. Candida antarctica lipase was extremely selective (E= 9 000) while Candida rugosa lipase was much less selective (E= 1.7). Other enzymes (Lipozyme and lipases from Pseudomonas and Rhizopus arrhizus) had intermediate selectivities. In all cases the enantioselectivity for an enzyme was unaffected by changes in water activity. Different methods of determining the enantioselectivity was used: reactions using single enantiomers as well as racemic mixtures. The effect of water activity on enantioselectivity and the enanatioselectivity values themselves were similar irrespective of the method used. The enantioselectivity of other alcohols were also found to be unaffected by the water activity. The enantioselectivity of Pseudomonas lipase was influenced by the organic solvent. The E decreased with increasing hydrophobicity, from 62 in acetonitrile to 40 in toluene and 33 in hexane. In none of these cases was the enantioselectivity affected by the water activity. However, for Lipozyme and Candida rugosa lipase in toluene a trend of increased E with increasing water activity was observed. In summary it can be stated that the water activity does not generally affect the enantioselectivity of the five lipases tested.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Alcohol resolution, Enantioselective esterification, Lipases, Water activity
in
Journal of Molecular Catalysis - B Enzymatic
volume
3
issue
5
pages
10 pages
publisher
Elsevier
external identifiers
  • scopus:0031558401
ISSN
1381-1177
DOI
10.1016/S1381-1177(97)00003-9
language
English
LU publication?
yes
id
3c828615-da85-41e3-9c4f-5d900e591b31
date added to LUP
2019-06-20 16:15:04
date last changed
2020-03-11 08:08:04
@article{3c828615-da85-41e3-9c4f-5d900e591b31,
  abstract     = {<p>The enantioselectivity (E) of lipases in esterifications of secondary alcohols with decanoic acid was studied in organic media. The enantioselectivity of 2-octanol differed greatly among the lipases used. Candida antarctica lipase was extremely selective (E= 9 000) while Candida rugosa lipase was much less selective (E= 1.7). Other enzymes (Lipozyme and lipases from Pseudomonas and Rhizopus arrhizus) had intermediate selectivities. In all cases the enantioselectivity for an enzyme was unaffected by changes in water activity. Different methods of determining the enantioselectivity was used: reactions using single enantiomers as well as racemic mixtures. The effect of water activity on enantioselectivity and the enanatioselectivity values themselves were similar irrespective of the method used. The enantioselectivity of other alcohols were also found to be unaffected by the water activity. The enantioselectivity of Pseudomonas lipase was influenced by the organic solvent. The E decreased with increasing hydrophobicity, from 62 in acetonitrile to 40 in toluene and 33 in hexane. In none of these cases was the enantioselectivity affected by the water activity. However, for Lipozyme and Candida rugosa lipase in toluene a trend of increased E with increasing water activity was observed. In summary it can be stated that the water activity does not generally affect the enantioselectivity of the five lipases tested.</p>},
  author       = {Wehtje, Ernst and Costes, David and Adlercreutz, Patrick},
  issn         = {1381-1177},
  language     = {eng},
  month        = {08},
  number       = {5},
  pages        = {221--230},
  publisher    = {Elsevier},
  series       = {Journal of Molecular Catalysis - B Enzymatic},
  title        = {Enantioselectivity of lipases : Effects of water activity},
  url          = {http://dx.doi.org/10.1016/S1381-1177(97)00003-9},
  doi          = {10.1016/S1381-1177(97)00003-9},
  volume       = {3},
  year         = {1997},
}