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Characterization of the genome, proteome, and structure of yersiniophage φR1-37

Skurnik, Mikael ; Hyytiäinen, Heidi J. ; Happonen, Lotta J. LU ; Kiljunen, Saija ; Datta, Neeta ; Mattinen, Laura ; Williamson, Kirsty ; Kristo, Paula ; Szeliga, Magdalena and Kalin-Mänttäri, Laura , et al. (2012) In Journal of Virology 86(23). p.12625-12642
Abstract

The bacteriophage vB_YecM-φR1-37 (φR1-37) is a lytic yersiniophage that can propagate naturally in different Yersinia species carrying the correct lipopolysaccharide receptor. This large-tailed phage has deoxyuridine (dU) instead of thymidine in its DNA. In this study, we determined the genomic sequence of phage φR1-37, mapped parts of the phage transcriptome, characterized the phage particle proteome, and characterized the virion structure by cryo-electron microscopy and image reconstruction. The 262,391-bp genome of φR1-37 is one of the largest sequenced phage genomes, and it contains 367 putative open reading frames (ORFs) and 5 tRNA genes. Mass-spectrometric analysis identified 69 phage particle structural proteins with the genes... (More)

The bacteriophage vB_YecM-φR1-37 (φR1-37) is a lytic yersiniophage that can propagate naturally in different Yersinia species carrying the correct lipopolysaccharide receptor. This large-tailed phage has deoxyuridine (dU) instead of thymidine in its DNA. In this study, we determined the genomic sequence of phage φR1-37, mapped parts of the phage transcriptome, characterized the phage particle proteome, and characterized the virion structure by cryo-electron microscopy and image reconstruction. The 262,391-bp genome of φR1-37 is one of the largest sequenced phage genomes, and it contains 367 putative open reading frames (ORFs) and 5 tRNA genes. Mass-spectrometric analysis identified 69 phage particle structural proteins with the genes scattered throughout the genome. A total of 269 of the ORFs (73%) lack homologues in sequence databases. Based on terminator and promoter sequences identified from the intergenic regions, the phage genome was predicted to consist of 40 to 60 transcriptional units. Image reconstruction revealed that the φR1-37 capsid consists of hexameric capsomers arranged on a T=27 lattice similar to the bacteriophage φKZ. The tail of φR1-37 has a contractile sheath. We conclude that phage φR1-37 is a representative of a novel phage type that carries the dU-containing genome in a φKZ-like head.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Virology
volume
86
issue
23
pages
18 pages
publisher
American Society for Microbiology
external identifiers
  • scopus:84869216595
  • pmid:22973030
ISSN
0022-538X
DOI
10.1128/JVI.01783-12
language
English
LU publication?
yes
additional info
Copyright 2013 Elsevier B.V., All rights reserved. Copyright © 2012, American Society for Microbiology
id
3cd2b664-0bb8-4472-9d52-15b10b0e1c62
date added to LUP
2021-03-22 09:58:02
date last changed
2024-03-08 10:44:32
@article{3cd2b664-0bb8-4472-9d52-15b10b0e1c62,
  abstract     = {{<p>The bacteriophage vB_YecM-φR1-37 (φR1-37) is a lytic yersiniophage that can propagate naturally in different Yersinia species carrying the correct lipopolysaccharide receptor. This large-tailed phage has deoxyuridine (dU) instead of thymidine in its DNA. In this study, we determined the genomic sequence of phage φR1-37, mapped parts of the phage transcriptome, characterized the phage particle proteome, and characterized the virion structure by cryo-electron microscopy and image reconstruction. The 262,391-bp genome of φR1-37 is one of the largest sequenced phage genomes, and it contains 367 putative open reading frames (ORFs) and 5 tRNA genes. Mass-spectrometric analysis identified 69 phage particle structural proteins with the genes scattered throughout the genome. A total of 269 of the ORFs (73%) lack homologues in sequence databases. Based on terminator and promoter sequences identified from the intergenic regions, the phage genome was predicted to consist of 40 to 60 transcriptional units. Image reconstruction revealed that the φR1-37 capsid consists of hexameric capsomers arranged on a T=27 lattice similar to the bacteriophage φKZ. The tail of φR1-37 has a contractile sheath. We conclude that phage φR1-37 is a representative of a novel phage type that carries the dU-containing genome in a φKZ-like head.</p>}},
  author       = {{Skurnik, Mikael and Hyytiäinen, Heidi J. and Happonen, Lotta J. and Kiljunen, Saija and Datta, Neeta and Mattinen, Laura and Williamson, Kirsty and Kristo, Paula and Szeliga, Magdalena and Kalin-Mänttäri, Laura and Ahola-Iivarinen, Elina and Kalkkinen, Nisse and Butcher, Sarah J.}},
  issn         = {{0022-538X}},
  language     = {{eng}},
  number       = {{23}},
  pages        = {{12625--12642}},
  publisher    = {{American Society for Microbiology}},
  series       = {{Journal of Virology}},
  title        = {{Characterization of the genome, proteome, and structure of yersiniophage φR1-37}},
  url          = {{http://dx.doi.org/10.1128/JVI.01783-12}},
  doi          = {{10.1128/JVI.01783-12}},
  volume       = {{86}},
  year         = {{2012}},
}