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The host defense peptide LL-37 is detected in human parotid and submandibular/sublingual saliva and expressed in glandular neutrophils

Svensson, Daniel LU ; Aidoukovitch, Alexandra LU ; Anders, Emma LU ; Agerberth, Birgitta; Andersson, Fredrik LU ; Ekblad, Eva LU ; Ericson, Dan; Nebel, Daniel LU ; Voss, Ulrikke LU and Nilsson, Bengt Olof LU (2018) In European Journal of Oral Sciences
Abstract

The human host defense peptide, LL-37, is an important player in the first line of defense against invading microorganisms. LL-37 and its precursor, hCAP18, have been detected in unstimulated whole saliva but no reports showing hCAP18/LL-37 in isolated, parotid, and/or submandibular/sublingual saliva have been presented. Here, we measured the levels of hCAP18/LL-37 in human parotid and submandibular/sublingual saliva and investigated the expression of hCAP18/LL-37 in parotid and submandibular gland tissue. Parotid and submandibular/sublingual saliva was collected from healthy volunteers, and the levels of hCAP18/LL-37 in saliva were analyzed by dot blot, ELISA, and western blotting. Cellular expression of hCAP18/LL-37 in human parotid... (More)

The human host defense peptide, LL-37, is an important player in the first line of defense against invading microorganisms. LL-37 and its precursor, hCAP18, have been detected in unstimulated whole saliva but no reports showing hCAP18/LL-37 in isolated, parotid, and/or submandibular/sublingual saliva have been presented. Here, we measured the levels of hCAP18/LL-37 in human parotid and submandibular/sublingual saliva and investigated the expression of hCAP18/LL-37 in parotid and submandibular gland tissue. Parotid and submandibular/sublingual saliva was collected from healthy volunteers, and the levels of hCAP18/LL-37 in saliva were analyzed by dot blot, ELISA, and western blotting. Cellular expression of hCAP18/LL-37 in human parotid and submandibular glands was investigated by immunohistochemistry. Immunoreactivity for hCAP18/LL-37 was detected in both parotid and submandibular/sublingual saliva of all individuals. The concentration of hCAP18/LL-37 was similar in parotid and submandibular/sublingual saliva, and was determined by densitometric scanning of each dot and normalization to the total protein concentration of each sample, and by ELISA. Double immunohistochemistry revealed that intravascular neutrophils of both parotid and submandibular glands express hCAP18/LL-37. For the first time, we demonstrate hCAP18/LL-37 in isolated human parotid and submandibular/sublingual saliva and expression of hCAP18/LL-37 in glandular intravascular neutrophils, indicating that neutrophils of the major salivary glands contribute to the LL-37 content of whole saliva.

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author
organization
publishing date
type
Contribution to journal
publication status
epub
subject
keywords
Antimicrobial peptide, Cathelicidin, HCAP18, Innate immune system, Salivary glands
in
European Journal of Oral Sciences
publisher
Wiley-Blackwell
external identifiers
  • scopus:85041740926
ISSN
0909-8836
DOI
10.1111/eos.12407
language
English
LU publication?
yes
id
3cf7ef6c-f60c-4b87-a3dd-4986ec803a72
date added to LUP
2018-02-21 13:20:16
date last changed
2018-05-29 09:57:29
@article{3cf7ef6c-f60c-4b87-a3dd-4986ec803a72,
  abstract     = {<p>The human host defense peptide, LL-37, is an important player in the first line of defense against invading microorganisms. LL-37 and its precursor, hCAP18, have been detected in unstimulated whole saliva but no reports showing hCAP18/LL-37 in isolated, parotid, and/or submandibular/sublingual saliva have been presented. Here, we measured the levels of hCAP18/LL-37 in human parotid and submandibular/sublingual saliva and investigated the expression of hCAP18/LL-37 in parotid and submandibular gland tissue. Parotid and submandibular/sublingual saliva was collected from healthy volunteers, and the levels of hCAP18/LL-37 in saliva were analyzed by dot blot, ELISA, and western blotting. Cellular expression of hCAP18/LL-37 in human parotid and submandibular glands was investigated by immunohistochemistry. Immunoreactivity for hCAP18/LL-37 was detected in both parotid and submandibular/sublingual saliva of all individuals. The concentration of hCAP18/LL-37 was similar in parotid and submandibular/sublingual saliva, and was determined by densitometric scanning of each dot and normalization to the total protein concentration of each sample, and by ELISA. Double immunohistochemistry revealed that intravascular neutrophils of both parotid and submandibular glands express hCAP18/LL-37. For the first time, we demonstrate hCAP18/LL-37 in isolated human parotid and submandibular/sublingual saliva and expression of hCAP18/LL-37 in glandular intravascular neutrophils, indicating that neutrophils of the major salivary glands contribute to the LL-37 content of whole saliva.</p>},
  author       = {Svensson, Daniel and Aidoukovitch, Alexandra and Anders, Emma and Agerberth, Birgitta and Andersson, Fredrik and Ekblad, Eva and Ericson, Dan and Nebel, Daniel and Voss, Ulrikke and Nilsson, Bengt Olof},
  issn         = {0909-8836},
  keyword      = {Antimicrobial peptide,Cathelicidin,HCAP18,Innate immune system,Salivary glands},
  language     = {eng},
  month        = {02},
  publisher    = {Wiley-Blackwell},
  series       = {European Journal of Oral Sciences},
  title        = {The host defense peptide LL-37 is detected in human parotid and submandibular/sublingual saliva and expressed in glandular neutrophils},
  url          = {http://dx.doi.org/10.1111/eos.12407},
  year         = {2018},
}