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Site-specific glycations of apolipoprotein A-I lead to differentiated functional effects on lipid-binding and on glucose metabolism

Domingo-Espín, Joan LU ; Nilsson, Oktawia LU ; Bernfur, Katja LU ; Del Giudice, Rita LU and Lagerstedt, Jens O. LU (2018) In Biochimica et Biophysica Acta - Molecular Basis of Disease 1864(9). p.2822-2834
Abstract

Prolonged hyperglycemia in poorly controlled diabetes leads to an increase in reactive glucose metabolites that covalently modify proteins by non-enzymatic glycation reactions. Apolipoprotein A-I (apoA-I) of high-density lipoprotein (HDL) is one of the proteins that becomes glycated in hyperglycemia. The impact of glycation on apoA-I protein structure and function in lipid and glucose metabolism were investigated. ApoA-I was chemically glycated by two different glucose metabolites (methylglyoxal and glycolaldehyde). Synchrotron radiation and conventional circular dichroism spectroscopy were used to study apoA-I structure and stability. The ability to bind lipids was measured by lipid-clearance assay and native gel analysis, and... (More)

Prolonged hyperglycemia in poorly controlled diabetes leads to an increase in reactive glucose metabolites that covalently modify proteins by non-enzymatic glycation reactions. Apolipoprotein A-I (apoA-I) of high-density lipoprotein (HDL) is one of the proteins that becomes glycated in hyperglycemia. The impact of glycation on apoA-I protein structure and function in lipid and glucose metabolism were investigated. ApoA-I was chemically glycated by two different glucose metabolites (methylglyoxal and glycolaldehyde). Synchrotron radiation and conventional circular dichroism spectroscopy were used to study apoA-I structure and stability. The ability to bind lipids was measured by lipid-clearance assay and native gel analysis, and cholesterol efflux was measured by using lipid-laden J774 macrophages. Diet induced obese mice with established insulin resistance, L6 rat and C2C12 mouse myocytes, as well as INS-1E rat insulinoma cells, were used to determine in vivo and in vitro glucose uptake and insulin secretion. Site-specific, covalent modifications of apoA-I (lysines or arginines) led to altered protein structure, reduced lipid binding capability and a reduced ability to catalyze cholesterol efflux from macrophages, partly in a modification-specific manner. The stimulatory effects of apoA-I on the in vivo glucose clearance were negatively affected when apoA-I was modified with methylglyoxal, but not with glycolaldehyde. The in vitro data showed that both glucose uptake in muscle cells and insulin secretion from beta cells were affected. Taken together, glycation modifications impair the apoA-I protein functionality in lipid and glucose metabolism, which is expected to have implications for diabetes patients with poorly controlled blood glucose.

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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
apoA-I, Diabetes, Glucose metabolism, Glycation, HDL, High-density lipoprotein
in
Biochimica et Biophysica Acta - Molecular Basis of Disease
volume
1864
issue
9
pages
2822 - 2834
publisher
Elsevier
external identifiers
  • scopus:85047779549
  • pmid:29802959
ISSN
0925-4439
DOI
10.1016/j.bbadis.2018.05.014
language
English
LU publication?
yes
id
3da2b011-fa59-48c9-b952-214aded67e86
date added to LUP
2018-06-14 16:18:19
date last changed
2024-04-01 07:10:02
@article{3da2b011-fa59-48c9-b952-214aded67e86,
  abstract     = {{<p>Prolonged hyperglycemia in poorly controlled diabetes leads to an increase in reactive glucose metabolites that covalently modify proteins by non-enzymatic glycation reactions. Apolipoprotein A-I (apoA-I) of high-density lipoprotein (HDL) is one of the proteins that becomes glycated in hyperglycemia. The impact of glycation on apoA-I protein structure and function in lipid and glucose metabolism were investigated. ApoA-I was chemically glycated by two different glucose metabolites (methylglyoxal and glycolaldehyde). Synchrotron radiation and conventional circular dichroism spectroscopy were used to study apoA-I structure and stability. The ability to bind lipids was measured by lipid-clearance assay and native gel analysis, and cholesterol efflux was measured by using lipid-laden J774 macrophages. Diet induced obese mice with established insulin resistance, L6 rat and C2C12 mouse myocytes, as well as INS-1E rat insulinoma cells, were used to determine in vivo and in vitro glucose uptake and insulin secretion. Site-specific, covalent modifications of apoA-I (lysines or arginines) led to altered protein structure, reduced lipid binding capability and a reduced ability to catalyze cholesterol efflux from macrophages, partly in a modification-specific manner. The stimulatory effects of apoA-I on the in vivo glucose clearance were negatively affected when apoA-I was modified with methylglyoxal, but not with glycolaldehyde. The in vitro data showed that both glucose uptake in muscle cells and insulin secretion from beta cells were affected. Taken together, glycation modifications impair the apoA-I protein functionality in lipid and glucose metabolism, which is expected to have implications for diabetes patients with poorly controlled blood glucose.</p>}},
  author       = {{Domingo-Espín, Joan and Nilsson, Oktawia and Bernfur, Katja and Del Giudice, Rita and Lagerstedt, Jens O.}},
  issn         = {{0925-4439}},
  keywords     = {{apoA-I; Diabetes; Glucose metabolism; Glycation; HDL; High-density lipoprotein}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{9}},
  pages        = {{2822--2834}},
  publisher    = {{Elsevier}},
  series       = {{Biochimica et Biophysica Acta - Molecular Basis of Disease}},
  title        = {{Site-specific glycations of apolipoprotein A-I lead to differentiated functional effects on lipid-binding and on glucose metabolism}},
  url          = {{http://dx.doi.org/10.1016/j.bbadis.2018.05.014}},
  doi          = {{10.1016/j.bbadis.2018.05.014}},
  volume       = {{1864}},
  year         = {{2018}},
}