Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Determination of the degree of branching in normal and amylopectin type potato starch with 1H-NMR spectroscopy : Improved resolution and two-dimensional spectroscopy

Nilsson, Gunilla S. LU ; Gorton, Lo LU ; Bergquist, Karl Erik LU and Nilsson, Ulf LU (1996) In Starch/Staerke 48(10). p.352-357
Abstract

Starch from genetically modified potatoes was found to be highly branched compared with normal potato varieties through the use of 1H-NMR spectroscopy. The average chain length, blue-value,and the wavelength at maximum absorptivity clearly show that the new potato varieties produce amylopectin starch. Correlation between the degree of branching as determined by 1H-NMR and starch-iodine complexation, expressed as blue-value, was good and the NMR-method gives low standard deviation. For the first time, the anomeric proton, H-I, of a (1→4)-α-linked D-glucose residue and the H-I of the glucose residue of a non-reducing end have been assigned separate chemical shifts in starch. Assignments were made as determined from... (More)

Starch from genetically modified potatoes was found to be highly branched compared with normal potato varieties through the use of 1H-NMR spectroscopy. The average chain length, blue-value,and the wavelength at maximum absorptivity clearly show that the new potato varieties produce amylopectin starch. Correlation between the degree of branching as determined by 1H-NMR and starch-iodine complexation, expressed as blue-value, was good and the NMR-method gives low standard deviation. For the first time, the anomeric proton, H-I, of a (1→4)-α-linked D-glucose residue and the H-I of the glucose residue of a non-reducing end have been assigned separate chemical shifts in starch. Assignments were made as determined from two-dimensional homonuclear and 1H-13C heteronuclear spectroscopy (COSY, HMQC, and HMBC). The molecular weight in degraded starch and pullulan were determined by means of NMR-spectroscopy. These results were in accordance with determinations by size exclusion chromatography and with the known molecular weights of pullulan standards.

(Less)
Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Starch/Staerke
volume
48
issue
10
pages
6 pages
publisher
John Wiley & Sons Inc.
external identifiers
  • scopus:0001744778
ISSN
0038-9056
DOI
10.1002/star.19960481003
language
English
LU publication?
yes
id
3e7b49fc-9071-4300-bc85-3bf304811c27
date added to LUP
2023-02-07 09:17:23
date last changed
2023-02-22 12:58:41
@article{3e7b49fc-9071-4300-bc85-3bf304811c27,
  abstract     = {{<p>Starch from genetically modified potatoes was found to be highly branched compared with normal potato varieties through the use of <sup>1</sup>H-NMR spectroscopy. The average chain length, blue-value,and the wavelength at maximum absorptivity clearly show that the new potato varieties produce amylopectin starch. Correlation between the degree of branching as determined by <sup>1</sup>H-NMR and starch-iodine complexation, expressed as blue-value, was good and the NMR-method gives low standard deviation. For the first time, the anomeric proton, H-I, of a (1→4)-α-linked D-glucose residue and the H-I of the glucose residue of a non-reducing end have been assigned separate chemical shifts in starch. Assignments were made as determined from two-dimensional homonuclear and <sup>1</sup>H-<sup>13</sup>C heteronuclear spectroscopy (COSY, HMQC, and HMBC). The molecular weight in degraded starch and pullulan were determined by means of NMR-spectroscopy. These results were in accordance with determinations by size exclusion chromatography and with the known molecular weights of pullulan standards.</p>}},
  author       = {{Nilsson, Gunilla S. and Gorton, Lo and Bergquist, Karl Erik and Nilsson, Ulf}},
  issn         = {{0038-9056}},
  language     = {{eng}},
  number       = {{10}},
  pages        = {{352--357}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Starch/Staerke}},
  title        = {{Determination of the degree of branching in normal and amylopectin type potato starch with <sup>1</sup>H-NMR spectroscopy : Improved resolution and two-dimensional spectroscopy}},
  url          = {{http://dx.doi.org/10.1002/star.19960481003}},
  doi          = {{10.1002/star.19960481003}},
  volume       = {{48}},
  year         = {{1996}},
}