(99m)Tc-DTPA Uptake and Electrical Impedance Measurements in Verification of In Vivo Electropermeabilization Efficiency in Rat Muscle.
Grafström, Gustav LU ; Engström, Per LU ; Salford, Leif LU and Persson, Bertil R LU
(2006)
In Cancer Biotherapy & Radiopharmaceuticals
21(6).
p.623-635
- Abstract
- Objective: In vivo electropermeabilization of cell membranes in rat muscle tissue cause a significant decrease of the electrical impedance, in the frequency region of 1-10 kHz. We aimed to study how the Tc-99m-DTPA uptake in the electropermeabilized region correlates to the change of admittance Y = 1/absZ, where Z is the measured impedance. Methods: The electropermeabilization was performed in vivo by applying high-voltage (0.5-2 kV) short (0.1-2 ms) pulses through gold-plated needle electrodes in skeletal muscle. The impedance was measured before and after each electropermeabilization pulse. The uptake of Tc-99m-DTPA uptake in the electropermeabilized region was measured after 6 and 24 hours with a gamma camera. Results: The pulse shape... (More)
- Objective: In vivo electropermeabilization of cell membranes in rat muscle tissue cause a significant decrease of the electrical impedance, in the frequency region of 1-10 kHz. We aimed to study how the Tc-99m-DTPA uptake in the electropermeabilized region correlates to the change of admittance Y = 1/absZ, where Z is the measured impedance. Methods: The electropermeabilization was performed in vivo by applying high-voltage (0.5-2 kV) short (0.1-2 ms) pulses through gold-plated needle electrodes in skeletal muscle. The impedance was measured before and after each electropermeabilization pulse. The uptake of Tc-99m-DTPA uptake in the electropermeabilized region was measured after 6 and 24 hours with a gamma camera. Results: The pulse shape (square and exponential), duration, and amplitude of the applied electric field were varied, and electropermeabilization efficiency was evaluated using the various measurement modalities. Good correlations were found (correlation coefficient approximate to 0.9) between the Tc-99m-DTPA uptake in the electropermeabilized and control "region of interest" the admittance ratio Y (post-treatment)/Y (pretreatment), and charge displacement parameter Q. Conclusion: The electrical impedance measurements method can be utilized in clinical settings to verify the efficiency of electropermeabilization applied to chemotherapy and to power RNAi (RNA-interference) and DNA-plasmid transfection in vaccination, immunization, and gene-therapy. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/164618
- author
- Grafström, Gustav
LU
; Engström, Per
LU
; Salford, Leif
LU
and Persson, Bertil R
LU
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- admittance, electrical impedance, electroporation, cell membranes, Tc-99m-DTPA, electropermeabilization, gamma camera, clinical dosimetry, chemotherapy, in vivo transfection, RNAi (RNA-interference), DNA-plasmid transfection, vaccination, gene therapy
- in
- Cancer Biotherapy & Radiopharmaceuticals
- volume
- 21
- issue
- 6
- pages
- 623 - 635
- publisher
- Mary Ann Liebert, Inc.
- external identifiers
-
- wos:000243781800010
- scopus:33846611466
- pmid:17257078
- ISSN
- 1557-8852
- DOI
- 10.1089/cbr.2006.21.623
- language
- English
- LU publication?
- yes
- id
- 3ecc7fee-ae10-404a-bbbd-4438ec4f083a (old id 164618)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17257078&dopt=Abstract
- date added to LUP
- 2016-04-01 15:20:18
- date last changed
- 2022-01-28 04:54:07
@article{3ecc7fee-ae10-404a-bbbd-4438ec4f083a,
abstract = {{Objective: In vivo electropermeabilization of cell membranes in rat muscle tissue cause a significant decrease of the electrical impedance, in the frequency region of 1-10 kHz. We aimed to study how the Tc-99m-DTPA uptake in the electropermeabilized region correlates to the change of admittance Y = 1/absZ, where Z is the measured impedance. Methods: The electropermeabilization was performed in vivo by applying high-voltage (0.5-2 kV) short (0.1-2 ms) pulses through gold-plated needle electrodes in skeletal muscle. The impedance was measured before and after each electropermeabilization pulse. The uptake of Tc-99m-DTPA uptake in the electropermeabilized region was measured after 6 and 24 hours with a gamma camera. Results: The pulse shape (square and exponential), duration, and amplitude of the applied electric field were varied, and electropermeabilization efficiency was evaluated using the various measurement modalities. Good correlations were found (correlation coefficient approximate to 0.9) between the Tc-99m-DTPA uptake in the electropermeabilized and control "region of interest" the admittance ratio Y (post-treatment)/Y (pretreatment), and charge displacement parameter Q. Conclusion: The electrical impedance measurements method can be utilized in clinical settings to verify the efficiency of electropermeabilization applied to chemotherapy and to power RNAi (RNA-interference) and DNA-plasmid transfection in vaccination, immunization, and gene-therapy.}},
author = {{Grafström, Gustav and Engström, Per and Salford, Leif and Persson, Bertil R}},
issn = {{1557-8852}},
keywords = {{admittance; electrical impedance; electroporation; cell membranes; Tc-99m-DTPA; electropermeabilization; gamma camera; clinical dosimetry; chemotherapy; in vivo transfection; RNAi (RNA-interference); DNA-plasmid transfection; vaccination; gene therapy}},
language = {{eng}},
number = {{6}},
pages = {{623--635}},
publisher = {{Mary Ann Liebert, Inc.}},
series = {{Cancer Biotherapy & Radiopharmaceuticals}},
title = {{(99m)Tc-DTPA Uptake and Electrical Impedance Measurements in Verification of In Vivo Electropermeabilization Efficiency in Rat Muscle.}},
url = {{http://dx.doi.org/10.1089/cbr.2006.21.623}},
doi = {{10.1089/cbr.2006.21.623}},
volume = {{21}},
year = {{2006}},
}