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(99m)Tc-DTPA Uptake and Electrical Impedance Measurements in Verification of In Vivo Electropermeabilization Efficiency in Rat Muscle.

Grafström, Gustav LU ; Engström, Per LU ; Salford, Leif LU and Persson, Bertil R LU (2006) In Cancer Biotherapy & Radiopharmaceuticals 21(6). p.623-635
Abstract
Objective: In vivo electropermeabilization of cell membranes in rat muscle tissue cause a significant decrease of the electrical impedance, in the frequency region of 1-10 kHz. We aimed to study how the Tc-99m-DTPA uptake in the electropermeabilized region correlates to the change of admittance Y = 1/absZ, where Z is the measured impedance. Methods: The electropermeabilization was performed in vivo by applying high-voltage (0.5-2 kV) short (0.1-2 ms) pulses through gold-plated needle electrodes in skeletal muscle. The impedance was measured before and after each electropermeabilization pulse. The uptake of Tc-99m-DTPA uptake in the electropermeabilized region was measured after 6 and 24 hours with a gamma camera. Results: The pulse shape... (More)
Objective: In vivo electropermeabilization of cell membranes in rat muscle tissue cause a significant decrease of the electrical impedance, in the frequency region of 1-10 kHz. We aimed to study how the Tc-99m-DTPA uptake in the electropermeabilized region correlates to the change of admittance Y = 1/absZ, where Z is the measured impedance. Methods: The electropermeabilization was performed in vivo by applying high-voltage (0.5-2 kV) short (0.1-2 ms) pulses through gold-plated needle electrodes in skeletal muscle. The impedance was measured before and after each electropermeabilization pulse. The uptake of Tc-99m-DTPA uptake in the electropermeabilized region was measured after 6 and 24 hours with a gamma camera. Results: The pulse shape (square and exponential), duration, and amplitude of the applied electric field were varied, and electropermeabilization efficiency was evaluated using the various measurement modalities. Good correlations were found (correlation coefficient approximate to 0.9) between the Tc-99m-DTPA uptake in the electropermeabilized and control "region of interest" the admittance ratio Y (post-treatment)/Y (pretreatment), and charge displacement parameter Q. Conclusion: The electrical impedance measurements method can be utilized in clinical settings to verify the efficiency of electropermeabilization applied to chemotherapy and to power RNAi (RNA-interference) and DNA-plasmid transfection in vaccination, immunization, and gene-therapy. (Less)
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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
admittance, electrical impedance, electroporation, cell membranes, Tc-99m-DTPA, electropermeabilization, gamma camera, clinical dosimetry, chemotherapy, in vivo transfection, RNAi (RNA-interference), DNA-plasmid transfection, vaccination, gene therapy
in
Cancer Biotherapy & Radiopharmaceuticals
volume
21
issue
6
pages
623 - 635
publisher
Mary Ann Liebert, Inc.
external identifiers
  • wos:000243781800010
  • scopus:33846611466
  • pmid:17257078
ISSN
1557-8852
DOI
10.1089/cbr.2006.21.623
language
English
LU publication?
yes
id
3ecc7fee-ae10-404a-bbbd-4438ec4f083a (old id 164618)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17257078&dopt=Abstract
date added to LUP
2016-04-01 15:20:18
date last changed
2020-01-12 18:24:59
@article{3ecc7fee-ae10-404a-bbbd-4438ec4f083a,
  abstract     = {Objective: In vivo electropermeabilization of cell membranes in rat muscle tissue cause a significant decrease of the electrical impedance, in the frequency region of 1-10 kHz. We aimed to study how the Tc-99m-DTPA uptake in the electropermeabilized region correlates to the change of admittance Y = 1/absZ, where Z is the measured impedance. Methods: The electropermeabilization was performed in vivo by applying high-voltage (0.5-2 kV) short (0.1-2 ms) pulses through gold-plated needle electrodes in skeletal muscle. The impedance was measured before and after each electropermeabilization pulse. The uptake of Tc-99m-DTPA uptake in the electropermeabilized region was measured after 6 and 24 hours with a gamma camera. Results: The pulse shape (square and exponential), duration, and amplitude of the applied electric field were varied, and electropermeabilization efficiency was evaluated using the various measurement modalities. Good correlations were found (correlation coefficient approximate to 0.9) between the Tc-99m-DTPA uptake in the electropermeabilized and control "region of interest" the admittance ratio Y (post-treatment)/Y (pretreatment), and charge displacement parameter Q. Conclusion: The electrical impedance measurements method can be utilized in clinical settings to verify the efficiency of electropermeabilization applied to chemotherapy and to power RNAi (RNA-interference) and DNA-plasmid transfection in vaccination, immunization, and gene-therapy.},
  author       = {Grafström, Gustav and Engström, Per and Salford, Leif and Persson, Bertil R},
  issn         = {1557-8852},
  language     = {eng},
  number       = {6},
  pages        = {623--635},
  publisher    = {Mary Ann Liebert, Inc.},
  series       = {Cancer Biotherapy & Radiopharmaceuticals},
  title        = {(99m)Tc-DTPA Uptake and Electrical Impedance Measurements in Verification of In Vivo Electropermeabilization Efficiency in Rat Muscle.},
  url          = {http://dx.doi.org/10.1089/cbr.2006.21.623},
  doi          = {10.1089/cbr.2006.21.623},
  volume       = {21},
  year         = {2006},
}