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Classical conditioning of Purkinje cell responses in vitro produces in vivo-like simple spike suppressions during the conditional stimulus

Gornov, Artem LU ; Moulin, Thiago C LU and Johansson, Fredrik (2026) In Biochemistry and Biophysics Reports 45.
Abstract

An in vitro model of classical conditioning could improve our understanding of underlying mechanisms thanks to controlled pharmacological manipulation and negligeable perturbations from external networks. Here, using mice cerebellar slices and patch clamp recordings, we present a protocol for classical conditioning of Purkinje cells in vitro at room temperature. Repeated pairing of a parallel fiber conditional stimulus and a climbing fiber unconditional stimulus with parameters compatible with in vivo learning gives rise to a Purkinje cell simple spike suppression during the conditional stimulus that shares similarities with conditioned responses observed in live animals.

Please use this url to cite or link to this publication:
author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biochemistry and Biophysics Reports
volume
45
article number
102493
publisher
Elsevier
external identifiers
  • pmid:41694666
  • scopus:105029388479
ISSN
2405-5808
DOI
10.1016/j.bbrep.2026.102493
language
English
LU publication?
yes
additional info
© 2026 The Authors.
id
3f15f21d-5870-4a9b-8578-e920b0f6d64c
date added to LUP
2026-09-02 08:57:33
date last changed
2026-09-03 04:01:27
@article{3f15f21d-5870-4a9b-8578-e920b0f6d64c,
  abstract     = {{<p>An in vitro model of classical conditioning could improve our understanding of underlying mechanisms thanks to controlled pharmacological manipulation and negligeable perturbations from external networks. Here, using mice cerebellar slices and patch clamp recordings, we present a protocol for classical conditioning of Purkinje cells in vitro at room temperature. Repeated pairing of a parallel fiber conditional stimulus and a climbing fiber unconditional stimulus with parameters compatible with in vivo learning gives rise to a Purkinje cell simple spike suppression during the conditional stimulus that shares similarities with conditioned responses observed in live animals.</p>}},
  author       = {{Gornov, Artem and Moulin, Thiago C and Johansson, Fredrik}},
  issn         = {{2405-5808}},
  language     = {{eng}},
  publisher    = {{Elsevier}},
  series       = {{Biochemistry and Biophysics Reports}},
  title        = {{Classical conditioning of Purkinje cell responses in vitro produces in vivo-like simple spike suppressions during the conditional stimulus}},
  url          = {{http://dx.doi.org/10.1016/j.bbrep.2026.102493}},
  doi          = {{10.1016/j.bbrep.2026.102493}},
  volume       = {{45}},
  year         = {{2026}},
}