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Spontaneous in vitro immunoglobulin secretion at the diagnosis of insulin-dependent diabetes

Papadopoulos, G. ; Petersen, J. ; Andersen, V. ; Lernmark, A. LU orcid ; Marner, B. ; Nerup, J. LU and Binder, C. (1984) In Acta Endocrinologica 105(4). p.521-527
Abstract

Blood mononuclear cells obtained from 17 newly diagnosed insulin-dependent diabetic (IDDM) patients treated with insulin for 5-7 days were assessed for the number of spontaneous and pokeweed mitogen (PWM)-stimulated immunoglobulin-secreting cells in a reverse haemolytic plaque assay. The spontaneous in vitro immunoglobulin secretion was evanescent and decreased in individual patients within 1-4 months of insulin treatment. Compared to matched controls, 53% (9/17) of the IDDM patients had an elevated spontaneous secretion of immunoglobulin, 41% (7/17) for IgG, 35% (6/17) for IgM, and 35% (6/17) for IgA. The quantities of PWM-stimulated IgG, IgM, or IgA secreting cells in IDDM were comparable to the controls. The IDDM patients with... (More)

Blood mononuclear cells obtained from 17 newly diagnosed insulin-dependent diabetic (IDDM) patients treated with insulin for 5-7 days were assessed for the number of spontaneous and pokeweed mitogen (PWM)-stimulated immunoglobulin-secreting cells in a reverse haemolytic plaque assay. The spontaneous in vitro immunoglobulin secretion was evanescent and decreased in individual patients within 1-4 months of insulin treatment. Compared to matched controls, 53% (9/17) of the IDDM patients had an elevated spontaneous secretion of immunoglobulin, 41% (7/17) for IgG, 35% (6/17) for IgM, and 35% (6/17) for IgA. The quantities of PWM-stimulated IgG, IgM, or IgA secreting cells in IDDM were comparable to the controls. The IDDM patients with spontaneous immunoglobulin secreting cells had higher fasting C-peptide levels compared to the patients with immunoglobulin-producing cells within the normal range (P < 0.05). The average titre of islet cell cytoplasmic antibodies was 1:26 in (9 out of 9 were positive) patients with, compared to 1:1 in patients (4 out of 8 were positive) without spontaneous secretion (P = 0.025). These results suggest that the clinical onset of IDDM is associated with a polyclonal B lymphocyte activation and that higher levels of fasting C-peptide islet cell antibodies are associated with this immunoregulatory abnormality.

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author
; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
in
Acta Endocrinologica
volume
105
issue
4
pages
7 pages
publisher
Society of the European Journal of Endocrinology
external identifiers
  • pmid:6372349
  • scopus:0021325119
ISSN
0001-5598
language
English
LU publication?
no
id
3fc67cf8-66c8-4536-ac37-736b0bbcffa9
date added to LUP
2019-09-16 12:42:06
date last changed
2024-03-13 08:00:10
@article{3fc67cf8-66c8-4536-ac37-736b0bbcffa9,
  abstract     = {{<p>Blood mononuclear cells obtained from 17 newly diagnosed insulin-dependent diabetic (IDDM) patients treated with insulin for 5-7 days were assessed for the number of spontaneous and pokeweed mitogen (PWM)-stimulated immunoglobulin-secreting cells in a reverse haemolytic plaque assay. The spontaneous in vitro immunoglobulin secretion was evanescent and decreased in individual patients within 1-4 months of insulin treatment. Compared to matched controls, 53% (9/17) of the IDDM patients had an elevated spontaneous secretion of immunoglobulin, 41% (7/17) for IgG, 35% (6/17) for IgM, and 35% (6/17) for IgA. The quantities of PWM-stimulated IgG, IgM, or IgA secreting cells in IDDM were comparable to the controls. The IDDM patients with spontaneous immunoglobulin secreting cells had higher fasting C-peptide levels compared to the patients with immunoglobulin-producing cells within the normal range (P &lt; 0.05). The average titre of islet cell cytoplasmic antibodies was 1:26 in (9 out of 9 were positive) patients with, compared to 1:1 in patients (4 out of 8 were positive) without spontaneous secretion (P = 0.025). These results suggest that the clinical onset of IDDM is associated with a polyclonal B lymphocyte activation and that higher levels of fasting C-peptide islet cell antibodies are associated with this immunoregulatory abnormality.</p>}},
  author       = {{Papadopoulos, G. and Petersen, J. and Andersen, V. and Lernmark, A. and Marner, B. and Nerup, J. and Binder, C.}},
  issn         = {{0001-5598}},
  language     = {{eng}},
  month        = {{05}},
  number       = {{4}},
  pages        = {{521--527}},
  publisher    = {{Society of the European Journal of Endocrinology}},
  series       = {{Acta Endocrinologica}},
  title        = {{Spontaneous in vitro immunoglobulin secretion at the diagnosis of insulin-dependent diabetes}},
  volume       = {{105}},
  year         = {{1984}},
}