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Heterogeneity among septic shock patients in a set of immunoregulatory markers.

Janols, Helena LU ; Wullt, Marlene LU ; Bergenfelz, Caroline LU ; Björnsson, Steinarr LU ; Lickei, H; Janciauskiene, Sabina LU ; Leandersson, Karin LU and Bredberg, Anders LU (2014) In European Journal of Clinical Microbiology & Infectious Diseases 33(3). p.313-324
Abstract
Immune activation is a regular feature of sepsis, but the incidence and nature of the ensuing inflammation-resolving and immunosuppressive component is less well understood. In this study, we compared immunoregulatory markers on blood leukocytes from patients with Gram-negative or Gram-positive sepsis or septic shock, and compared this to blood from patients with severe virosis or healthy controls. To this end, blood from 32 patients with sepsis, including ten cases with shock, and 12 patients with severe virosis were analysed by flow cytometry for the expression levels of monocyte HLA-DR, CD11c, CD14 and CD40, and for frequencies of CD163(+)-suppressive monocytes, HLA-DR(+) or CD40(+)-activated T cells and Tregs. Plasma cytokine levels... (More)
Immune activation is a regular feature of sepsis, but the incidence and nature of the ensuing inflammation-resolving and immunosuppressive component is less well understood. In this study, we compared immunoregulatory markers on blood leukocytes from patients with Gram-negative or Gram-positive sepsis or septic shock, and compared this to blood from patients with severe virosis or healthy controls. To this end, blood from 32 patients with sepsis, including ten cases with shock, and 12 patients with severe virosis were analysed by flow cytometry for the expression levels of monocyte HLA-DR, CD11c, CD14 and CD40, and for frequencies of CD163(+)-suppressive monocytes, HLA-DR(+) or CD40(+)-activated T cells and Tregs. Plasma cytokine levels were analysed as a functional measurement. Signs of immunosuppression dominated in the septic shock and Gram-positive sepsis groups, whereas monocyte activation was common in Gram-negative sepsis patients without shock. However, the main finding was the large inter-individual variation of immune activation and immunosuppression, with no correlation to prognosis among the shock patients. The pronounced inter-individual variation in the analysed monocyte and lymphocyte markers forms a strong argument that, when immunomodulatory treatment is considered in a sepsis patient, it should be personalised and guided by a detailed immune status assessment. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
European Journal of Clinical Microbiology & Infectious Diseases
volume
33
issue
3
pages
313 - 324
publisher
Springer
external identifiers
  • pmid:23990136
  • wos:000331709700004
  • scopus:84896708263
ISSN
1435-4373
DOI
10.1007/s10096-013-1957-y
language
English
LU publication?
yes
id
47cbcc53-ea9d-4b4a-86d7-ab3e1408a6e5 (old id 4005246)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/23990136?dopt=Abstract
date added to LUP
2013-09-05 14:12:00
date last changed
2017-07-02 03:11:32
@article{47cbcc53-ea9d-4b4a-86d7-ab3e1408a6e5,
  abstract     = {Immune activation is a regular feature of sepsis, but the incidence and nature of the ensuing inflammation-resolving and immunosuppressive component is less well understood. In this study, we compared immunoregulatory markers on blood leukocytes from patients with Gram-negative or Gram-positive sepsis or septic shock, and compared this to blood from patients with severe virosis or healthy controls. To this end, blood from 32 patients with sepsis, including ten cases with shock, and 12 patients with severe virosis were analysed by flow cytometry for the expression levels of monocyte HLA-DR, CD11c, CD14 and CD40, and for frequencies of CD163(+)-suppressive monocytes, HLA-DR(+) or CD40(+)-activated T cells and Tregs. Plasma cytokine levels were analysed as a functional measurement. Signs of immunosuppression dominated in the septic shock and Gram-positive sepsis groups, whereas monocyte activation was common in Gram-negative sepsis patients without shock. However, the main finding was the large inter-individual variation of immune activation and immunosuppression, with no correlation to prognosis among the shock patients. The pronounced inter-individual variation in the analysed monocyte and lymphocyte markers forms a strong argument that, when immunomodulatory treatment is considered in a sepsis patient, it should be personalised and guided by a detailed immune status assessment.},
  author       = {Janols, Helena and Wullt, Marlene and Bergenfelz, Caroline and Björnsson, Steinarr and Lickei, H and Janciauskiene, Sabina and Leandersson, Karin and Bredberg, Anders},
  issn         = {1435-4373},
  language     = {eng},
  number       = {3},
  pages        = {313--324},
  publisher    = {Springer},
  series       = {European Journal of Clinical Microbiology & Infectious Diseases},
  title        = {Heterogeneity among septic shock patients in a set of immunoregulatory markers.},
  url          = {http://dx.doi.org/10.1007/s10096-013-1957-y},
  volume       = {33},
  year         = {2014},
}