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A network-based analysis of the late-phase reaction of the skin

Benson, Mikael ; Langston, Michael A. ; Adner, Mikael LU ; Andersson, Bengt ; Torinssson-Naluai, Asa and Cardell, Lars-Olaf LU (2006) In Journal of Allergy and Clinical Immunology 118(1). p.220-225
Abstract
Background: The late-phase reaction (LPR) of the skin is an in vivo model of allergic inflammation. Objective: We sought to identify disease-associated pathways in the LPR using a network-based analysis. Methods: The LPR was examined by means of DNA microarray analysis of skin biopsy specimens from 10 patients with allergic rhinitis and 10 healthy control subjects. The results were further analyzed in 2 different materials consisting of nasal fluids and allergen-challenged CD4(+) T cells from patients with allergic rhinitis. Results: The DNA microarray analysis revealed several genes of known relevance to allergy. The eosinophil marker Charcot-Leyden crystal protein (CLC) that encodes Charcot-Leyden crystal protein differed most in... (More)
Background: The late-phase reaction (LPR) of the skin is an in vivo model of allergic inflammation. Objective: We sought to identify disease-associated pathways in the LPR using a network-based analysis. Methods: The LPR was examined by means of DNA microarray analysis of skin biopsy specimens from 10 patients with allergic rhinitis and 10 healthy control subjects. The results were further analyzed in 2 different materials consisting of nasal fluids and allergen-challenged CD4(+) T cells from patients with allergic rhinitis. Results: The DNA microarray analysis revealed several genes of known relevance to allergy. The eosinophil marker Charcot-Leyden crystal protein (CLC) that encodes Charcot-Leyden crystal protein differed most in expression. A network-based analysis showed upregulation of IL-4- and CCL4-dependent pathways and downregulation of a TGF-beta-induced pathway. CCL4 is expressed by CD4(+) T cells and chemotactic for eosinophils. We hypothesized that allergen induces release of CCL4 from TH2 cells and that this contributes to influx of eosinophils. Further analysis showed increase of CCL4 protein in nasal fluids from allergic patients during the season. Allergen challenge of PBMCs resulted in proliferation of TH2 cells and increased production of CCL4 in CD4(+) T cells from allergic patients. An analysis of the DNA microarray data revealed a significant correlation between CCL4 and the eosinophil marker CLC. Conclusion: A network-based analysis of the LPR showed increased activity of IL-4- and CCL4-dependent pathways and downregulation of the TGF-beta-induced pathway. Allergen-induced release of CCL4 from T(H)2 cells might contribute to influx of eosinophils during the LPR. Clinical implications: Involvement of multiple interacting pathways indicates that it might be difficult to identify one single mediator as a biomarker or drug target in allergic inflammation. (Less)
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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
late-phase reaction, DNA microarrays, gene expression
in
Journal of Allergy and Clinical Immunology
volume
118
issue
1
pages
220 - 225
publisher
Elsevier
external identifiers
  • pmid:16815159
  • wos:000239184800027
  • scopus:33745376748
ISSN
1097-6825
DOI
10.1016/j.jaci.2006.03.006
language
English
LU publication?
yes
id
e7091f04-8ed2-4313-aa9d-fc88ebce7844 (old id 401355)
date added to LUP
2016-04-01 16:18:59
date last changed
2021-08-04 04:12:23
@article{e7091f04-8ed2-4313-aa9d-fc88ebce7844,
  abstract     = {Background: The late-phase reaction (LPR) of the skin is an in vivo model of allergic inflammation. Objective: We sought to identify disease-associated pathways in the LPR using a network-based analysis. Methods: The LPR was examined by means of DNA microarray analysis of skin biopsy specimens from 10 patients with allergic rhinitis and 10 healthy control subjects. The results were further analyzed in 2 different materials consisting of nasal fluids and allergen-challenged CD4(+) T cells from patients with allergic rhinitis. Results: The DNA microarray analysis revealed several genes of known relevance to allergy. The eosinophil marker Charcot-Leyden crystal protein (CLC) that encodes Charcot-Leyden crystal protein differed most in expression. A network-based analysis showed upregulation of IL-4- and CCL4-dependent pathways and downregulation of a TGF-beta-induced pathway. CCL4 is expressed by CD4(+) T cells and chemotactic for eosinophils. We hypothesized that allergen induces release of CCL4 from TH2 cells and that this contributes to influx of eosinophils. Further analysis showed increase of CCL4 protein in nasal fluids from allergic patients during the season. Allergen challenge of PBMCs resulted in proliferation of TH2 cells and increased production of CCL4 in CD4(+) T cells from allergic patients. An analysis of the DNA microarray data revealed a significant correlation between CCL4 and the eosinophil marker CLC. Conclusion: A network-based analysis of the LPR showed increased activity of IL-4- and CCL4-dependent pathways and downregulation of the TGF-beta-induced pathway. Allergen-induced release of CCL4 from T(H)2 cells might contribute to influx of eosinophils during the LPR. Clinical implications: Involvement of multiple interacting pathways indicates that it might be difficult to identify one single mediator as a biomarker or drug target in allergic inflammation.},
  author       = {Benson, Mikael and Langston, Michael A. and Adner, Mikael and Andersson, Bengt and Torinssson-Naluai, Asa and Cardell, Lars-Olaf},
  issn         = {1097-6825},
  language     = {eng},
  number       = {1},
  pages        = {220--225},
  publisher    = {Elsevier},
  series       = {Journal of Allergy and Clinical Immunology},
  title        = {A network-based analysis of the late-phase reaction of the skin},
  url          = {http://dx.doi.org/10.1016/j.jaci.2006.03.006},
  doi          = {10.1016/j.jaci.2006.03.006},
  volume       = {118},
  year         = {2006},
}