Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Interaction of fungal laccases and laccase-mediator systems with lignin

Shleev, Sergey LU ; Persson, Per LU ; Shumakovich, Galina ; Mazhugo, Yulia ; Yaropolov, Alexander ; Ruzgas, Tautgirdas LU and Gorton, Lo LU (2006) In Enzyme and Microbial Technology 39(4). p.841-847
Abstract
The interaction between fungal laccases from Trametes basidiomycetes and different lignins has been studied using electrochemical and chromatographic methods. The kinetic parameters for the oxidation reaction of hydroquinone and for Kraft lignin by molecular oxygen catalysed by Trametes hirsuta laccase have been investigated. Polymerisation of lignin by laccase was shown. The process was independent on the source of lignin as well as on the origin and purity of the enzymes. A complex pattern of interaction between the laccase-mediator system (LMS) and lignin was shown. Along with the polymerisation of lignin, the ability to degrade lignin using the LMS was observed. Data generated by combining size exclusion chromatographic separation of... (More)
The interaction between fungal laccases from Trametes basidiomycetes and different lignins has been studied using electrochemical and chromatographic methods. The kinetic parameters for the oxidation reaction of hydroquinone and for Kraft lignin by molecular oxygen catalysed by Trametes hirsuta laccase have been investigated. Polymerisation of lignin by laccase was shown. The process was independent on the source of lignin as well as on the origin and purity of the enzymes. A complex pattern of interaction between the laccase-mediator system (LMS) and lignin was shown. Along with the polymerisation of lignin, the ability to degrade lignin using the LMS was observed. Data generated by combining size exclusion chromatographic separation of lignin with the use of a laccase modified electrode as post column detector, strongly suggest that laccase can oxidise natural lignin by a long-range electron transfer mechanism. The influence of the origin of lignin as well as source of the enzyme on this process was also shown. (c) 2006 Elsevier Inc. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
amperometry, chromatography, biosensor, laccase, lignin, mediator
in
Enzyme and Microbial Technology
volume
39
issue
4
pages
841 - 847
publisher
Elsevier
external identifiers
  • wos:000238932500049
  • scopus:33745195044
ISSN
0141-0229
DOI
10.1016/j.enzmictec.2006.01.010
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
id
08ff7fbb-5b22-41f2-a8a5-599102fa0ca5 (old id 402235)
date added to LUP
2016-04-01 12:33:46
date last changed
2021-02-17 04:42:05
@article{08ff7fbb-5b22-41f2-a8a5-599102fa0ca5,
  abstract     = {The interaction between fungal laccases from Trametes basidiomycetes and different lignins has been studied using electrochemical and chromatographic methods. The kinetic parameters for the oxidation reaction of hydroquinone and for Kraft lignin by molecular oxygen catalysed by Trametes hirsuta laccase have been investigated. Polymerisation of lignin by laccase was shown. The process was independent on the source of lignin as well as on the origin and purity of the enzymes. A complex pattern of interaction between the laccase-mediator system (LMS) and lignin was shown. Along with the polymerisation of lignin, the ability to degrade lignin using the LMS was observed. Data generated by combining size exclusion chromatographic separation of lignin with the use of a laccase modified electrode as post column detector, strongly suggest that laccase can oxidise natural lignin by a long-range electron transfer mechanism. The influence of the origin of lignin as well as source of the enzyme on this process was also shown. (c) 2006 Elsevier Inc. All rights reserved.},
  author       = {Shleev, Sergey and Persson, Per and Shumakovich, Galina and Mazhugo, Yulia and Yaropolov, Alexander and Ruzgas, Tautgirdas and Gorton, Lo},
  issn         = {0141-0229},
  language     = {eng},
  number       = {4},
  pages        = {841--847},
  publisher    = {Elsevier},
  series       = {Enzyme and Microbial Technology},
  title        = {Interaction of fungal laccases and laccase-mediator systems with lignin},
  url          = {http://dx.doi.org/10.1016/j.enzmictec.2006.01.010},
  doi          = {10.1016/j.enzmictec.2006.01.010},
  volume       = {39},
  year         = {2006},
}