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Size characterization of green fluorescent protein inclusion bodies in E. coli using asymmetrical flow field-flow fractionation-multi-angle light scattering

Luo, J ; Leeman, Mats LU ; Ballagi, A ; Elfwing, A ; Su, ZG ; Janson, JC and Wahlund, Karl-Gustav LU (2006) 29th International Symposium on High Performance Liquid Phase Separations and Related Techniques - Part II 1120(1-2). p.158-164
Abstract
The goal of this study was to investigate the applicability of asymmetrical flow field-flow fractionation-multi-angle light scattering (AsFIFFFMALS) for size analysis of green fluorescent protein inclusion bodies (GFPIBs). The size distributions of GFPIBs prepared by various culture conditions were determined. For GFPIBs prepared at 37 degrees C the peak maximum hydrodynamic diameter (dH) first increased and then decreased with the increase of the induction times in the presence of 0.1 and 2 mM isopropyl-beta-D-thiogalactoside (IPTG). For GFPIBs prepared at 30 degrees C the peak maximum dH was constant at about 700 nm irrespectively of the induction times and IPTG concentrations.
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author
; ; ; ; ; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
hydrodynamic diameter, fluorescence protein inclusion bodies, green, multi-angle light, asymmetrical flow field-flow fractionation, scattering, root mean square radius
host publication
Journal of Chromatography A
volume
1120
issue
1-2
pages
158 - 164
publisher
Elsevier
conference name
29th International Symposium on High Performance Liquid Phase Separations and Related Techniques - Part II
conference location
Stockholm, Sweden
conference dates
2005-06-26 - 2005-06-30
external identifiers
  • pmid:16387315
  • wos:000238623400020
  • scopus:33744981348
ISSN
0021-9673
DOI
10.1016/j.chroma.2005.11.048
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
id
1ec94f7c-3397-44f3-b1f9-c48dfff87612 (old id 404900)
date added to LUP
2016-04-01 16:22:47
date last changed
2021-09-22 05:04:15
@inproceedings{1ec94f7c-3397-44f3-b1f9-c48dfff87612,
  abstract     = {The goal of this study was to investigate the applicability of asymmetrical flow field-flow fractionation-multi-angle light scattering (AsFIFFFMALS) for size analysis of green fluorescent protein inclusion bodies (GFPIBs). The size distributions of GFPIBs prepared by various culture conditions were determined. For GFPIBs prepared at 37 degrees C the peak maximum hydrodynamic diameter (dH) first increased and then decreased with the increase of the induction times in the presence of 0.1 and 2 mM isopropyl-beta-D-thiogalactoside (IPTG). For GFPIBs prepared at 30 degrees C the peak maximum dH was constant at about 700 nm irrespectively of the induction times and IPTG concentrations.},
  author       = {Luo, J and Leeman, Mats and Ballagi, A and Elfwing, A and Su, ZG and Janson, JC and Wahlund, Karl-Gustav},
  booktitle    = {Journal of Chromatography A},
  issn         = {0021-9673},
  language     = {eng},
  number       = {1-2},
  pages        = {158--164},
  publisher    = {Elsevier},
  title        = {Size characterization of green fluorescent protein inclusion bodies in E. coli using asymmetrical flow field-flow fractionation-multi-angle light scattering},
  url          = {http://dx.doi.org/10.1016/j.chroma.2005.11.048},
  doi          = {10.1016/j.chroma.2005.11.048},
  volume       = {1120},
  year         = {2006},
}