Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Formate dehydrogenase-modified carbon paste electrodes for amperometric detection of formate

Munteanu, Florentina LU ; Gligor, D ; Popescu, IC and Gorton, Lo LU (2006) In Revue Roumaine de Chimie 51(1). p.25-30
Abstract
A simple and inexpensive amperometric biosensor for formate detection was obtained by immobilization of for-mate dehydrogenase (FDH) on graphite powder, modified with a new phenothiazine derivative (DDDP; 16H, 18H-dibenzo[c,l]-7,9-dithia-16,18-diazapentacene). Two different enzyme environments were used: FDH was adsorbed on DDDP-modified graphite powder in the presence of polyethylenimine (PEI) as well as in its absence. In both cases, when 10 mM NAD(+) were added in the supporting electrolyte (phosphate buffer, pH 7.0), the biosensor developed a response to formate, obeying Michaelis-Menten kinetics with a linear domain up to 3 mM formate. The biosensor sensitivity, calculated as the ratio 1(max)/K-M(aPP), was better in the second case... (More)
A simple and inexpensive amperometric biosensor for formate detection was obtained by immobilization of for-mate dehydrogenase (FDH) on graphite powder, modified with a new phenothiazine derivative (DDDP; 16H, 18H-dibenzo[c,l]-7,9-dithia-16,18-diazapentacene). Two different enzyme environments were used: FDH was adsorbed on DDDP-modified graphite powder in the presence of polyethylenimine (PEI) as well as in its absence. In both cases, when 10 mM NAD(+) were added in the supporting electrolyte (phosphate buffer, pH 7.0), the biosensor developed a response to formate, obeying Michaelis-Menten kinetics with a linear domain up to 3 mM formate. The biosensor sensitivity, calculated as the ratio 1(max)/K-M(aPP), was better in the second case (0.962 mA/M), but the best detection limit (3 AM) was observed in the first case. (Less)
Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Revue Roumaine de Chimie
volume
51
issue
1
pages
25 - 30
publisher
Romanian Academy / Academia Române
external identifiers
  • wos:000238234900004
  • scopus:33745014881
ISSN
0035-3930
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
id
2b7bcf0a-d1ab-44c8-9fa0-85d8f8a86350 (old id 405825)
date added to LUP
2016-04-01 16:23:05
date last changed
2021-06-23 04:04:08
@article{2b7bcf0a-d1ab-44c8-9fa0-85d8f8a86350,
  abstract     = {A simple and inexpensive amperometric biosensor for formate detection was obtained by immobilization of for-mate dehydrogenase (FDH) on graphite powder, modified with a new phenothiazine derivative (DDDP; 16H, 18H-dibenzo[c,l]-7,9-dithia-16,18-diazapentacene). Two different enzyme environments were used: FDH was adsorbed on DDDP-modified graphite powder in the presence of polyethylenimine (PEI) as well as in its absence. In both cases, when 10 mM NAD(+) were added in the supporting electrolyte (phosphate buffer, pH 7.0), the biosensor developed a response to formate, obeying Michaelis-Menten kinetics with a linear domain up to 3 mM formate. The biosensor sensitivity, calculated as the ratio 1(max)/K-M(aPP), was better in the second case (0.962 mA/M), but the best detection limit (3 AM) was observed in the first case.},
  author       = {Munteanu, Florentina and Gligor, D and Popescu, IC and Gorton, Lo},
  issn         = {0035-3930},
  language     = {eng},
  number       = {1},
  pages        = {25--30},
  publisher    = {Romanian Academy / Academia Române},
  series       = {Revue Roumaine de Chimie},
  title        = {Formate dehydrogenase-modified carbon paste electrodes for amperometric detection of formate},
  volume       = {51},
  year         = {2006},
}