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Characterization of insulin microcrystals using powder diffraction and multivariate data analysis

Norrman, Mathias LU ; Stahl, K ; Schluckebier, G and Al-Karadaghi, Salam LU (2006) In Journal of Applied Crystallography 39. p.391-400
Abstract
Twelve different microcrystalline insulin formulations were investigated by X-ray powder diffraction and were shown to have very characteristic patterns. Three of the formulations crystallize in the same crystal system, but have structural differences in the N-terminal B-chain of the insulin molecule. This difference was efficiently detected in the powder patterns. The sensitivity of the method makes it a valuable tool for characterization of microcrystalline samples. By use of principal-component analysis, the twelve different formulations originating from six different crystal systems were classified into nine separate clusters. The powder patterns of each cluster can now be used as `fingerprints' for the different insulin polymorphs.... (More)
Twelve different microcrystalline insulin formulations were investigated by X-ray powder diffraction and were shown to have very characteristic patterns. Three of the formulations crystallize in the same crystal system, but have structural differences in the N-terminal B-chain of the insulin molecule. This difference was efficiently detected in the powder patterns. The sensitivity of the method makes it a valuable tool for characterization of microcrystalline samples. By use of principal-component analysis, the twelve different formulations originating from six different crystal systems were classified into nine separate clusters. The powder patterns of each cluster can now be used as `fingerprints' for the different insulin polymorphs. The combination of X-ray powder diffraction and multivariate analysis, such as principal-component analysis, provides a rapid and effective tool for studying the influence of derivatives, additives, ions, pH etc., in the crystallization media. (Less)
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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Applied Crystallography
volume
39
pages
391 - 400
publisher
International Union of Crystallography
external identifiers
  • wos:000237461400012
  • scopus:33744454406
ISSN
1600-5767
DOI
10.1107/S0021889806011058
language
English
LU publication?
yes
id
50dba17a-cbc2-42a7-b57a-3ca069d08480 (old id 409518)
date added to LUP
2016-04-01 12:38:47
date last changed
2022-01-27 07:55:57
@article{50dba17a-cbc2-42a7-b57a-3ca069d08480,
  abstract     = {{Twelve different microcrystalline insulin formulations were investigated by X-ray powder diffraction and were shown to have very characteristic patterns. Three of the formulations crystallize in the same crystal system, but have structural differences in the N-terminal B-chain of the insulin molecule. This difference was efficiently detected in the powder patterns. The sensitivity of the method makes it a valuable tool for characterization of microcrystalline samples. By use of principal-component analysis, the twelve different formulations originating from six different crystal systems were classified into nine separate clusters. The powder patterns of each cluster can now be used as `fingerprints' for the different insulin polymorphs. The combination of X-ray powder diffraction and multivariate analysis, such as principal-component analysis, provides a rapid and effective tool for studying the influence of derivatives, additives, ions, pH etc., in the crystallization media.}},
  author       = {{Norrman, Mathias and Stahl, K and Schluckebier, G and Al-Karadaghi, Salam}},
  issn         = {{1600-5767}},
  language     = {{eng}},
  pages        = {{391--400}},
  publisher    = {{International Union of Crystallography}},
  series       = {{Journal of Applied Crystallography}},
  title        = {{Characterization of insulin microcrystals using powder diffraction and multivariate data analysis}},
  url          = {{http://dx.doi.org/10.1107/S0021889806011058}},
  doi          = {{10.1107/S0021889806011058}},
  volume       = {{39}},
  year         = {{2006}},
}