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Characterization of insulin microcrystals using powder diffraction and multivariate data analysis

Norrman, Mathias LU ; Stahl, K ; Schluckebier, G and Al-Karadaghi, Salam LU (2006) In Journal of Applied Crystallography 39. p.391-400
Abstract
Twelve different microcrystalline insulin formulations were investigated by X-ray powder diffraction and were shown to have very characteristic patterns. Three of the formulations crystallize in the same crystal system, but have structural differences in the N-terminal B-chain of the insulin molecule. This difference was efficiently detected in the powder patterns. The sensitivity of the method makes it a valuable tool for characterization of microcrystalline samples. By use of principal-component analysis, the twelve different formulations originating from six different crystal systems were classified into nine separate clusters. The powder patterns of each cluster can now be used as `fingerprints' for the different insulin polymorphs.... (More)
Twelve different microcrystalline insulin formulations were investigated by X-ray powder diffraction and were shown to have very characteristic patterns. Three of the formulations crystallize in the same crystal system, but have structural differences in the N-terminal B-chain of the insulin molecule. This difference was efficiently detected in the powder patterns. The sensitivity of the method makes it a valuable tool for characterization of microcrystalline samples. By use of principal-component analysis, the twelve different formulations originating from six different crystal systems were classified into nine separate clusters. The powder patterns of each cluster can now be used as `fingerprints' for the different insulin polymorphs. The combination of X-ray powder diffraction and multivariate analysis, such as principal-component analysis, provides a rapid and effective tool for studying the influence of derivatives, additives, ions, pH etc., in the crystallization media. (Less)
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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Applied Crystallography
volume
39
pages
391 - 400
publisher
International Union of Crystallography
external identifiers
  • wos:000237461400012
  • scopus:33744454406
ISSN
1600-5767
DOI
10.1107/S0021889806011058
language
English
LU publication?
yes
id
50dba17a-cbc2-42a7-b57a-3ca069d08480 (old id 409518)
date added to LUP
2016-04-01 12:38:47
date last changed
2021-08-11 02:41:26
@article{50dba17a-cbc2-42a7-b57a-3ca069d08480,
  abstract     = {Twelve different microcrystalline insulin formulations were investigated by X-ray powder diffraction and were shown to have very characteristic patterns. Three of the formulations crystallize in the same crystal system, but have structural differences in the N-terminal B-chain of the insulin molecule. This difference was efficiently detected in the powder patterns. The sensitivity of the method makes it a valuable tool for characterization of microcrystalline samples. By use of principal-component analysis, the twelve different formulations originating from six different crystal systems were classified into nine separate clusters. The powder patterns of each cluster can now be used as `fingerprints' for the different insulin polymorphs. The combination of X-ray powder diffraction and multivariate analysis, such as principal-component analysis, provides a rapid and effective tool for studying the influence of derivatives, additives, ions, pH etc., in the crystallization media.},
  author       = {Norrman, Mathias and Stahl, K and Schluckebier, G and Al-Karadaghi, Salam},
  issn         = {1600-5767},
  language     = {eng},
  pages        = {391--400},
  publisher    = {International Union of Crystallography},
  series       = {Journal of Applied Crystallography},
  title        = {Characterization of insulin microcrystals using powder diffraction and multivariate data analysis},
  url          = {http://dx.doi.org/10.1107/S0021889806011058},
  doi          = {10.1107/S0021889806011058},
  volume       = {39},
  year         = {2006},
}