Phenolic compounds from Syzygium jambos (Myrtaceae) exhibit distinct antioxidant and hepatoprotective activities in vivo
(2018) In Journal of Functional Foods 41. p.223-231- Abstract
HPLC-PDA-MS/MS analysis of Syzygium jambos leaf extract allowed the identification of 17 secondary metabolites, including flavonol glycosides, flavonol di-glycosides, and flavones as well as ellagitannins and phenolic acids. The extract showed promising antioxidant activities in different experimental models. In Caenorhabditis elegans, the extract mediated a dose dependent survival rate and decreased the intracellular ROS level and HSP-16.2 expression. In a rat model against acute CCl4 intoxication, the extract reduced the levels of all tested liver markers ALT, AST, TB, TC, TG, and MDA and increased GSH and SOD; effects were similar to those of silymarin. In hepatocytes, pre-treatment with the extract inhibited ROS... (More)
HPLC-PDA-MS/MS analysis of Syzygium jambos leaf extract allowed the identification of 17 secondary metabolites, including flavonol glycosides, flavonol di-glycosides, and flavones as well as ellagitannins and phenolic acids. The extract showed promising antioxidant activities in different experimental models. In Caenorhabditis elegans, the extract mediated a dose dependent survival rate and decreased the intracellular ROS level and HSP-16.2 expression. In a rat model against acute CCl4 intoxication, the extract reduced the levels of all tested liver markers ALT, AST, TB, TC, TG, and MDA and increased GSH and SOD; effects were similar to those of silymarin. In hepatocytes, pre-treatment with the extract inhibited ROS production, against the deleterious effects of sodium arsenite, and increased GSH levels, without alteration in the phosphorylation levels of p38 and of its direct target, MAPKAPK-2. These results demonstrate the high efficacy of S. jambos extract in free radical-scavenging, and inhibition of reactive oxygen species.
(Less)
- author
- Sobeh, Mansour
; Esmat, Ahmed
; Petruk, Ganna
LU
; Abdelfattah, Mohamed A.O. ; Dmirieh, Malak ; Monti, Daria Maria ; Abdel-Naim, Ashraf B. and Wink, Michael
- publishing date
- 2018-02
- type
- Contribution to journal
- publication status
- published
- keywords
- Antioxidant, Caenorhabditis elegans, Hepatoprotective, HPLC-PDA-ESI-MS/MS, In vivo, Syzygium jambos
- in
- Journal of Functional Foods
- volume
- 41
- pages
- 223 - 231
- publisher
- Elsevier
- external identifiers
-
- scopus:85039458385
- ISSN
- 1756-4646
- DOI
- 10.1016/j.jff.2017.12.055
- language
- English
- LU publication?
- no
- additional info
- Publisher Copyright: © 2017 Elsevier Ltd
- id
- 40d12c6d-9b7b-49e7-b7f7-d6949efe09d8
- date added to LUP
- 2025-01-21 14:45:33
- date last changed
- 2025-01-22 08:22:01
@article{40d12c6d-9b7b-49e7-b7f7-d6949efe09d8, abstract = {{<p>HPLC-PDA-MS/MS analysis of Syzygium jambos leaf extract allowed the identification of 17 secondary metabolites, including flavonol glycosides, flavonol di-glycosides, and flavones as well as ellagitannins and phenolic acids. The extract showed promising antioxidant activities in different experimental models. In Caenorhabditis elegans, the extract mediated a dose dependent survival rate and decreased the intracellular ROS level and HSP-16.2 expression. In a rat model against acute CCl<sub>4</sub> intoxication, the extract reduced the levels of all tested liver markers ALT, AST, TB, TC, TG, and MDA and increased GSH and SOD; effects were similar to those of silymarin. In hepatocytes, pre-treatment with the extract inhibited ROS production, against the deleterious effects of sodium arsenite, and increased GSH levels, without alteration in the phosphorylation levels of p38 and of its direct target, MAPKAPK-2. These results demonstrate the high efficacy of S. jambos extract in free radical-scavenging, and inhibition of reactive oxygen species.</p>}}, author = {{Sobeh, Mansour and Esmat, Ahmed and Petruk, Ganna and Abdelfattah, Mohamed A.O. and Dmirieh, Malak and Monti, Daria Maria and Abdel-Naim, Ashraf B. and Wink, Michael}}, issn = {{1756-4646}}, keywords = {{Antioxidant; Caenorhabditis elegans; Hepatoprotective; HPLC-PDA-ESI-MS/MS; In vivo; Syzygium jambos}}, language = {{eng}}, pages = {{223--231}}, publisher = {{Elsevier}}, series = {{Journal of Functional Foods}}, title = {{Phenolic compounds from Syzygium jambos (Myrtaceae) exhibit distinct antioxidant and hepatoprotective activities in vivo}}, url = {{http://dx.doi.org/10.1016/j.jff.2017.12.055}}, doi = {{10.1016/j.jff.2017.12.055}}, volume = {{41}}, year = {{2018}}, }