Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

MTA1, a transcriptional activator of breast cancer amplified sequence 3

Gururaj, AE ; Singh, RR ; Rayala, SK ; Wigerup, Caroline LU ; den Hollander, P ; Zhang, H ; Balasenthil, S ; Talukder, AH ; Landberg, Göran LU and Kumar, R (2006) In Proceedings of the National Academy of Sciences 103(17). p.6670-6675
Abstract
Here we define a function of metastasis-associated protein 1 (MTA1), a presumed corepressor of estrogen receptor alpha (ER alpha), as a transcriptional activator of Breast Cancer Amplified Sequence 3 (BCAS3), a gene amplified and overexpressed in breast cancers. We identified BCAS3 as a MTA1 chromatin target in a functional genomic screen. MTA1 stimulation of BCAS3 transcription required ERa and involved a functional ERE half-site in BCAS3. Furthermore, we discovered that MTA1 is acetylated on lysine 626, and that this acetylation is necessary for a productive transcriptional recruitment of RNA polymerase 11 complex to the BCAS3 enhancer sequence. BCAS3 expression was elevated in mammary tumors from MTA1 transgenic mice and 60% of the... (More)
Here we define a function of metastasis-associated protein 1 (MTA1), a presumed corepressor of estrogen receptor alpha (ER alpha), as a transcriptional activator of Breast Cancer Amplified Sequence 3 (BCAS3), a gene amplified and overexpressed in breast cancers. We identified BCAS3 as a MTA1 chromatin target in a functional genomic screen. MTA1 stimulation of BCAS3 transcription required ERa and involved a functional ERE half-site in BCAS3. Furthermore, we discovered that MTA1 is acetylated on lysine 626, and that this acetylation is necessary for a productive transcriptional recruitment of RNA polymerase 11 complex to the BCAS3 enhancer sequence. BCAS3 expression was elevated in mammary tumors from MTA1 transgenic mice and 60% of the human breast tumors, and correlated with the coexpression of MTA1 as well as with tumor grade and proliferation of primary breast tumor samples. These findings reveal a previously unrecognized function of MTA1 in stimulating BCAS3 expression and suggest an important role for MTA1-BCAS3 pathway in promoting cancerous phenotypes in breast tumor cells. (Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
estrogen receptor, BCAS3, coactivator
in
Proceedings of the National Academy of Sciences
volume
103
issue
17
pages
6670 - 6675
publisher
National Academy of Sciences
external identifiers
  • pmid:16617102
  • wos:000237151000046
  • scopus:33646238716
  • pmid:16617102
ISSN
1091-6490
DOI
10.1073/pnas.0601989103
language
English
LU publication?
yes
id
4d2badf6-1890-408d-8b5f-abad52f4c4b3 (old id 410255)
date added to LUP
2016-04-01 11:35:48
date last changed
2022-05-06 06:20:51
@article{4d2badf6-1890-408d-8b5f-abad52f4c4b3,
  abstract     = {{Here we define a function of metastasis-associated protein 1 (MTA1), a presumed corepressor of estrogen receptor alpha (ER alpha), as a transcriptional activator of Breast Cancer Amplified Sequence 3 (BCAS3), a gene amplified and overexpressed in breast cancers. We identified BCAS3 as a MTA1 chromatin target in a functional genomic screen. MTA1 stimulation of BCAS3 transcription required ERa and involved a functional ERE half-site in BCAS3. Furthermore, we discovered that MTA1 is acetylated on lysine 626, and that this acetylation is necessary for a productive transcriptional recruitment of RNA polymerase 11 complex to the BCAS3 enhancer sequence. BCAS3 expression was elevated in mammary tumors from MTA1 transgenic mice and 60% of the human breast tumors, and correlated with the coexpression of MTA1 as well as with tumor grade and proliferation of primary breast tumor samples. These findings reveal a previously unrecognized function of MTA1 in stimulating BCAS3 expression and suggest an important role for MTA1-BCAS3 pathway in promoting cancerous phenotypes in breast tumor cells.}},
  author       = {{Gururaj, AE and Singh, RR and Rayala, SK and Wigerup, Caroline and den Hollander, P and Zhang, H and Balasenthil, S and Talukder, AH and Landberg, Göran and Kumar, R}},
  issn         = {{1091-6490}},
  keywords     = {{estrogen receptor; BCAS3; coactivator}},
  language     = {{eng}},
  number       = {{17}},
  pages        = {{6670--6675}},
  publisher    = {{National Academy of Sciences}},
  series       = {{Proceedings of the National Academy of Sciences}},
  title        = {{MTA1, a transcriptional activator of breast cancer amplified sequence 3}},
  url          = {{http://dx.doi.org/10.1073/pnas.0601989103}},
  doi          = {{10.1073/pnas.0601989103}},
  volume       = {{103}},
  year         = {{2006}},
}