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The Na(+) transport in Gram-positive bacteria defect in the Mrp antiporter complex measured with (23)Na-NMR.

Gorecki, Kamil LU ; Hägerhäll, Cecilia LU and Drakenberg, Torbjörn LU (2014) In Analytical Biochemistry 445(Online 15 October 2013). p.80-86
Abstract
(23)Na-NMR has previously been used to monitor Na(+) translocation across membranes in Gram-negative bacteria and in various other organelles and liposomes using a membrane-impermeable shift reagent to resolve the signals resulting from internal and external Na(+). In this work, the (23)Na-NMR method was adapted for measurements of internal Na(+) concentration in the Gram-positive bacterium Bacillus subtilis, with the aim of assessing the Na(+) translocation activity of the Mrp antiporter complex, a member of the Cation Proton Antiporter-3 (CPA-3) family. The sodium sensitive growth phenotype observed in a B. subtilis strain with the gene encoding MrpA deleted, could indeed be correlated to the inability of this strain to maintain a lower... (More)
(23)Na-NMR has previously been used to monitor Na(+) translocation across membranes in Gram-negative bacteria and in various other organelles and liposomes using a membrane-impermeable shift reagent to resolve the signals resulting from internal and external Na(+). In this work, the (23)Na-NMR method was adapted for measurements of internal Na(+) concentration in the Gram-positive bacterium Bacillus subtilis, with the aim of assessing the Na(+) translocation activity of the Mrp antiporter complex, a member of the Cation Proton Antiporter-3 (CPA-3) family. The sodium sensitive growth phenotype observed in a B. subtilis strain with the gene encoding MrpA deleted, could indeed be correlated to the inability of this strain to maintain a lower internal than external Na(+) concentration. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Biochemistry
volume
445
issue
Online 15 October 2013
pages
80 - 86
publisher
Elsevier
external identifiers
  • wos:000328236800013
  • pmid:24139955
  • scopus:84888116310
ISSN
1096-0309
DOI
10.1016/j.ab.2013.10.003
language
English
LU publication?
yes
id
02d06514-0cde-46d3-adcb-b0fc71d6a03a (old id 4143164)
date added to LUP
2013-11-12 17:28:13
date last changed
2017-05-28 03:25:13
@article{02d06514-0cde-46d3-adcb-b0fc71d6a03a,
  abstract     = {(23)Na-NMR has previously been used to monitor Na(+) translocation across membranes in Gram-negative bacteria and in various other organelles and liposomes using a membrane-impermeable shift reagent to resolve the signals resulting from internal and external Na(+). In this work, the (23)Na-NMR method was adapted for measurements of internal Na(+) concentration in the Gram-positive bacterium Bacillus subtilis, with the aim of assessing the Na(+) translocation activity of the Mrp antiporter complex, a member of the Cation Proton Antiporter-3 (CPA-3) family. The sodium sensitive growth phenotype observed in a B. subtilis strain with the gene encoding MrpA deleted, could indeed be correlated to the inability of this strain to maintain a lower internal than external Na(+) concentration.},
  author       = {Gorecki, Kamil and Hägerhäll, Cecilia and Drakenberg, Torbjörn},
  issn         = {1096-0309},
  language     = {eng},
  number       = {Online 15 October 2013},
  pages        = {80--86},
  publisher    = {Elsevier},
  series       = {Analytical Biochemistry},
  title        = {The Na(+) transport in Gram-positive bacteria defect in the Mrp antiporter complex measured with (23)Na-NMR.},
  url          = {http://dx.doi.org/10.1016/j.ab.2013.10.003},
  volume       = {445},
  year         = {2014},
}