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Pressurised hot water extraction in continuous flow mode for thermolabile compounds: extraction of polyphenols in red onions.

Liu, Jiayin LU ; Sandahl, Margareta LU ; Sjöberg, Per J R and Turner, Charlotta LU (2014) In Analytical and Bioanalytical Chemistry 406(2). p.441-445
Abstract
Extraction and analysis of labile compounds in complex sample matrices, such as plants, is often a big analytical challenge. In this work, the use of a "green and clean" pressurised hot water extraction (PHWE) approach performed in continuous flow mode is explored. Experimental data for extraction and degradation kinetics of selected compounds were utilised to develop a continuous flow extraction (CFE) method targeting thermolabile polyphenols in red onions, with detection by high-performance liquid chromatography (HPLC)-diode array detection (DAD)-mass spectrometry (MS). Water containing ethanol and formic acid was used as extraction solvent. Method performance was focused on extraction yield with minimal analyte degradation. By adjusting... (More)
Extraction and analysis of labile compounds in complex sample matrices, such as plants, is often a big analytical challenge. In this work, the use of a "green and clean" pressurised hot water extraction (PHWE) approach performed in continuous flow mode is explored. Experimental data for extraction and degradation kinetics of selected compounds were utilised to develop a continuous flow extraction (CFE) method targeting thermolabile polyphenols in red onions, with detection by high-performance liquid chromatography (HPLC)-diode array detection (DAD)-mass spectrometry (MS). Water containing ethanol and formic acid was used as extraction solvent. Method performance was focused on extraction yield with minimal analyte degradation. By adjusting the flow rate of the extraction solvent, degradation effects were minimised, and complete extraction could be achieved within 60 min. The CFE extraction yields of the polyphenols investigated were 80-90 % of the theoretically calculated quantitative yields and were significantly higher than the yields obtained by conventional methanol extraction and static batch extraction (70-79 and 58-67 % of the theoretical yields, respectively). The precision of the developed method was lower than 8 % expressed as relative standard deviation. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical and Bioanalytical Chemistry
volume
406
issue
2
pages
441 - 445
publisher
Springer
external identifiers
  • wos:000329626600007
  • pmid:24091739
  • scopus:84899086790
ISSN
1618-2642
DOI
10.1007/s00216-013-7370-7
language
English
LU publication?
yes
id
ceccea91-6503-4150-bc41-883aa4525914 (old id 4143735)
date added to LUP
2013-11-12 18:14:48
date last changed
2017-10-01 03:22:32
@article{ceccea91-6503-4150-bc41-883aa4525914,
  abstract     = {Extraction and analysis of labile compounds in complex sample matrices, such as plants, is often a big analytical challenge. In this work, the use of a "green and clean" pressurised hot water extraction (PHWE) approach performed in continuous flow mode is explored. Experimental data for extraction and degradation kinetics of selected compounds were utilised to develop a continuous flow extraction (CFE) method targeting thermolabile polyphenols in red onions, with detection by high-performance liquid chromatography (HPLC)-diode array detection (DAD)-mass spectrometry (MS). Water containing ethanol and formic acid was used as extraction solvent. Method performance was focused on extraction yield with minimal analyte degradation. By adjusting the flow rate of the extraction solvent, degradation effects were minimised, and complete extraction could be achieved within 60 min. The CFE extraction yields of the polyphenols investigated were 80-90 % of the theoretically calculated quantitative yields and were significantly higher than the yields obtained by conventional methanol extraction and static batch extraction (70-79 and 58-67 % of the theoretical yields, respectively). The precision of the developed method was lower than 8 % expressed as relative standard deviation.},
  author       = {Liu, Jiayin and Sandahl, Margareta and Sjöberg, Per J R and Turner, Charlotta},
  issn         = {1618-2642},
  language     = {eng},
  number       = {2},
  pages        = {441--445},
  publisher    = {Springer},
  series       = {Analytical and Bioanalytical Chemistry},
  title        = {Pressurised hot water extraction in continuous flow mode for thermolabile compounds: extraction of polyphenols in red onions.},
  url          = {http://dx.doi.org/10.1007/s00216-013-7370-7},
  volume       = {406},
  year         = {2014},
}